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吴朝波, 王蕾, 郭建春, 符少萍, 刘姣, 李瑞梅, 江行玉, 段瑞军. 镉在海雀稗体内的分布及化学形态特征[J]. 环境化学, 2016, 35(2): 330-336. doi: 10.7524/j.issn.0254-6108.2016.02.2015090201
引用本文: 吴朝波, 王蕾, 郭建春, 符少萍, 刘姣, 李瑞梅, 江行玉, 段瑞军. 镉在海雀稗体内的分布及化学形态特征[J]. 环境化学, 2016, 35(2): 330-336. doi: 10.7524/j.issn.0254-6108.2016.02.2015090201
shu
WU Chaobo, WANG Lei, GUO Jianchun, FU Shaoping, LIU Jiao, LI Ruimei, JIANG Xingyu, DUAN Ruijun. Distribution and chemical forms of Cd in PASPALUM VAGINATUM SW.[J]. Environmental Chemistry, 2016, 35(2): 330-336. doi: 10.7524/j.issn.0254-6108.2016.02.2015090201
Citation: WU Chaobo, WANG Lei, GUO Jianchun, FU Shaoping, LIU Jiao, LI Ruimei, JIANG Xingyu, DUAN Ruijun. Distribution and chemical forms of Cd in PASPALUM VAGINATUM SW.[J]. Environmental Chemistry, 2016, 35(2): 330-336. doi: 10.7524/j.issn.0254-6108.2016.02.2015090201
shu

镉在海雀稗体内的分布及化学形态特征

  • 1.

    中国热带农业科学院/热带生物技术研究所, 海口, 571101;

  • 2.

    海南大学农学院, 海口, 570228;

  • 3.

    海南大学园艺园林学院, 海口, 570228

  • 基金项目:

    中央公益性科研院所基本科研业务专项基金(ITBB2015ZD03,1630052015038)

    海南省重大科技专项(ZDZX2013023-1)资助.

Distribution and chemical forms of Cd in PASPALUM VAGINATUM SW.

  • 1.

    Institute of Tropical Bioscience and Biotechnology, China Academy of Tropical Agricultural Science, Haikou, 571101, China;

  • 2.

    College of Agriculture, Hainan University, Haikou, 570228, China;

  • 3.

    College of Chemistry and Chemical Engineering, Hainan University, Haikou, 570228, China

  • Fund Project: Supported by the Central Level, Non-profit, Scientific Research Institutes for Basic R & D Operations Special Fund Program(ITBB2015ZD03, 1630052015038) Major Technology Project of Hainan(ZDZX2013023-1).

  • 摘要: 通过盆栽试验,研究了不同浓度(0、1、10、50、100、200 mg·kg-1)镉处理下,海雀稗地上部与地下部的生物量受镉胁迫的影响及其体内镉的富集特征,进一步分析低镉处理(1 mg·kg-1)和高镉处理(50 mg·kg-1)镉在海雀稗根、茎、叶中的化学形态及根、叶中镉的亚细胞分布特征.结果表明,镉浓度≤50 mg·kg-1,海雀稗地上部、地下部生物量和耐性系数都未显著下降,海雀稗根部对镉的积累远大于地上部分;海雀稗根系细胞壁中镉含量分配比例>50%,其次为可溶部分,高镉处理中细胞壁、可溶部分中镉的分配比例比低镉处理增加6.14%、0.32%,细胞膜部分和原生质体部分镉分配比例下降;海雀稗镉形态主要为氯化钠、醋酸提取态,高镉处理降低了毒性较高的水提取态和乙醇提取态的比例总和,根中氯化钠和盐酸提取态,茎、叶中醋酸提取态、氯化钠提取态增加.研究表明,海雀稗中毒性较低、活性较弱的盐酸提取态、醋酸提取态和氯化钠提取态Cd分配比例增加,根中细胞壁固持和液泡区隔化可能是海雀稗应对镉胁迫的重要耐性机制.
    Abstract: A pot experiment was conducted to investigate the effects of stress reponses of Cd and accumulation to Cd for the biomass in the root and leaf of Paspalum vaginatum Sw. with different Cd treatments(0、1、10、50、100、200 mg·kg-1). Further analysis was conducted for the chemical forms of Cd in the root, stem and leaf of Paspalum vaginatum Sw., and the subcellular distribution of Cd in root and leaf with two different levels of Cd treatment. The results showed that the biomass and tolerance index of the root and leaf in Paspalum vaginatum Sw. did not reduce significantly when Cd concentration in solution was below 50 mg·kg-1. Most of the accumulated Cd was enriched in the cell wall and soluble fraction. The proportion of Cd located in cell wall is above 50% with the treatment of 50 mg·kg-1. The proportion of Cd in cell wall of root, soluble fraction and cell wall of leaf was increased and the proportion of Cd in cytomembrane of root and leaf was reduced. The chemical forms. were FNaCl and FHAC. The Cd accumulation percentages in FNaCl and HAC extractable fractions decreased with the treatment of 50 mg·kg-1, whereas FNaCl and HCl in root and HAC and FNaCl in leaf were increased. It indicated that, the Cd accumulation percentages in FHCl, HAC and FNaCl extractable fractions increased. Therefore, cell wall binding of root and vacuolar compartmentalization were supposed to be the main tolerance mechanisms to Cd for Paspalum vaginatum Sw.. with Cd stress.
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  • 收稿日期:  2015-09-02
  • 刊出日期:  2016-02-15
吴朝波, 王蕾, 郭建春, 符少萍, 刘姣, 李瑞梅, 江行玉, 段瑞军. 镉在海雀稗体内的分布及化学形态特征[J]. 环境化学, 2016, 35(2): 330-336. doi: 10.7524/j.issn.0254-6108.2016.02.2015090201
引用本文: 吴朝波, 王蕾, 郭建春, 符少萍, 刘姣, 李瑞梅, 江行玉, 段瑞军. 镉在海雀稗体内的分布及化学形态特征[J]. 环境化学, 2016, 35(2): 330-336. doi: 10.7524/j.issn.0254-6108.2016.02.2015090201
shu
WU Chaobo, WANG Lei, GUO Jianchun, FU Shaoping, LIU Jiao, LI Ruimei, JIANG Xingyu, DUAN Ruijun. Distribution and chemical forms of Cd in PASPALUM VAGINATUM SW.[J]. Environmental Chemistry, 2016, 35(2): 330-336. doi: 10.7524/j.issn.0254-6108.2016.02.2015090201
Citation: WU Chaobo, WANG Lei, GUO Jianchun, FU Shaoping, LIU Jiao, LI Ruimei, JIANG Xingyu, DUAN Ruijun. Distribution and chemical forms of Cd in PASPALUM VAGINATUM SW.[J]. Environmental Chemistry, 2016, 35(2): 330-336. doi: 10.7524/j.issn.0254-6108.2016.02.2015090201
shu

镉在海雀稗体内的分布及化学形态特征

  • 1.  中国热带农业科学院/热带生物技术研究所, 海口, 571101;
  • 2.  海南大学农学院, 海口, 570228;
  • 3.  海南大学园艺园林学院, 海口, 570228
  • 收稿日期:  2015-09-02
基金项目:

中央公益性科研院所基本科研业务专项基金(ITBB2015ZD03,1630052015038)

海南省重大科技专项(ZDZX2013023-1)资助.

摘要: 通过盆栽试验,研究了不同浓度(0、1、10、50、100、200 mg·kg-1)镉处理下,海雀稗地上部与地下部的生物量受镉胁迫的影响及其体内镉的富集特征,进一步分析低镉处理(1 mg·kg-1)和高镉处理(50 mg·kg-1)镉在海雀稗根、茎、叶中的化学形态及根、叶中镉的亚细胞分布特征.结果表明,镉浓度≤50 mg·kg-1,海雀稗地上部、地下部生物量和耐性系数都未显著下降,海雀稗根部对镉的积累远大于地上部分;海雀稗根系细胞壁中镉含量分配比例>50%,其次为可溶部分,高镉处理中细胞壁、可溶部分中镉的分配比例比低镉处理增加6.14%、0.32%,细胞膜部分和原生质体部分镉分配比例下降;海雀稗镉形态主要为氯化钠、醋酸提取态,高镉处理降低了毒性较高的水提取态和乙醇提取态的比例总和,根中氯化钠和盐酸提取态,茎、叶中醋酸提取态、氯化钠提取态增加.研究表明,海雀稗中毒性较低、活性较弱的盐酸提取态、醋酸提取态和氯化钠提取态Cd分配比例增加,根中细胞壁固持和液泡区隔化可能是海雀稗应对镉胁迫的重要耐性机制.

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