聚苯乙烯微塑料暴露对剑尾鱼肝脏代谢通路的影响
Effects of Polystyrene Microplastics on Metabolism in Liver of Swordtail Fish
-
摘要: 微塑料已经成为水环境中的主要污染物，可能会对水生生物产生影响。将成年剑尾鱼暴露在直径5 μm、不同浓度的聚苯乙烯微塑料水体中72 h，检测急性暴露是否对剑尾鱼肝脏代谢产生干扰，以探讨微塑料的潜在毒性。结果表明，微塑料会导致剑尾鱼肝脏的代谢水平发生改变，在浓度为1×106 microspheres·L-1的低浓度组中，筛选出6种代谢水平发生改变的差异代谢物，共涉及16条代谢通路，这些代谢物与肝脏中的能量代谢、糖代谢、氨基酸代谢、炎症反应和氧化应激有关。在浓度为1×107 microspheres·L-1的高浓度组中，筛选出8种代谢水平发生改变的差异代谢物，共涉及19条代谢通路，这些代谢物与肝脏中的能量代谢、糖代谢、氨基酸代谢、炎症反应和氧化应激有关，并且脂代谢干扰与神经毒性有关的代谢物表达发生改变。从受干扰的代谢物的数量和代谢通路数量来看，微塑料浓度增加将会加深对水生生物的干扰。Abstract: Microplastics have become the main pollutants in the water environment and may impact aquatic organisms. In this paper, adult swordtail fish was exposed to polystyrene microplastics with a diameter of 5 μm for 72 h. The potential toxicity of microplastics was discussed by detecting whether acute exposure interfered with the metabolism of swordtail fish's liver. The results showed that microplastics caused changes in metabolism in the liver of swordtail fish. In the low concentration group of 1×106 microspheres·L-1, six different metabolites with altered metabolic levels were screened out, involving a total of 16 metabolic pathways. They are related to energy metabolism, sugar metabolism, amino acid metabolism, inflammatory reaction and oxidative stress in the liver. While in the high concentration experimental group with the concentration of 1×107 microspheres·L-1, eight different metabolites with altered metabolic levels were screened out, involving 19 metabolic pathways. These metabolites were related to energy metabolism, sugar metabolism, amino acid metabolism, inflammatory reaction and oxidative stress in the liver. Moreover, it was found that lipid metabolism interfered with the expression of metabolites related to neurotoxicity, which was different from the 1×106 microspheres·L-1 group. According to the number of disturbing metabolites and metabolic pathways, we can conclude that the increase of microplastics concentration will deepen the interference to aquatic organisms.
-
Key words:
- microplastics /
- Xiphophorus helleri /
- metabolomics /
- liver
-
-
Rochman C M, Browne M A, Halpern B S, et al. Policy:Classify plastic waste as hazardous[J]. Nature, 2013, 494(7436):169-171 de Sá L C, Oliveira M, Ribeiro F, et al. Studies of the effects of microplastics on aquatic organisms:What do we know and where should we focus our efforts in the future?[J]. The Science of the Total Environment, 2018, 645:1029-1039 Prata J C, Silva A L P, Walker T R, et al. COVID-19 pandemic repercussions on the use and management of plastics[J]. Environmental Science & Technology, 2020, 54(13):7760-7765 Andrady A L. Microplastics in the marine environment[J]. Marine Pollution Bulletin, 2011, 62(8):1596-1605 Thompson R C, Moore C J, vom Saal F S, et al. Plastics, the environment and human health:Current consensus and future trends[J]. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences, 2009, 364(1526):2153-2166 Takahashi K, Hosie G, Kitchener J, et al. Report on the Southern Ocean continuous plankton recorder (SO-CPR) standards workshop:SCAR expert group on CPR research[J]. Antarctic Record, 2011, 55(3):279-286 Kanhai D K, Gårdfeldt K, Lyashevska O, et al. Microplastics in sub-surface waters of the Arctic Central Basin[J]. Marine Pollution Bulletin, 2018, 130:8-18 Jamieson A J, Brooks L S R, Reid W D K, et al. Microplastics and synthetic particles ingested by deep-sea amphipods in six of the deepest marine ecosystems on Earth[J]. Royal Society Open Science, 2019, 6(2):180667 Napper I E, Davies B F R, Clifford H, et al. Reaching new heights in plastic pollution:Preliminary findings of microplastics on mount Everest[J]. One Earth, 2020, 3(5):621-630 Yang W F, Gao X X, Wu Y X, et al. The combined toxicity influence of microplastics and nonylphenol on microalgae Chlorella pyrenoidosa[J]. Ecotoxicology and Environmental Safety, 2020, 195:110484 李爱峰, 李方晓, 邱江兵, 等. 水环境中微塑料的污染现状、生物毒性及控制对策[J]. 中国海洋大学学报:自然科学版, 2019, 49(10):88-100 Li A F, Li F X, Qiu J B, et al. Pollution status, biological toxicity and control strategy of microplastics in water environments:A review[J]. Periodical of Ocean University of China, 2019, 49(10):88-100(in Chinese)
Yin L Y, Chen B J, Xia B, et al. Polystyrene microplastics alter the behavior, energy reserve and nutritional composition of marine jacopever (Sebastes schlegelii)[J]. Journal of Hazardous Materials, 2018, 360:97-105 Watts A J R, Lewis C, Goodhead R M, et al. Uptake and retention of microplastics by the shore crab Carcinus maenas[J]. Environmental Science & Technology, 2014, 48(15):8823-8830 Watts A J, Urbina M A, Goodhead R, et al. Effect of microplastic on the gills of the shore crab Carcinus maenas[J]. Environmental Science & Technology, 2016, 50(10):5364-5369 Pannetier P, Morin B, le Bihanic F, et al. Environmental samples of microplastics induce significant toxic effects in fish larvae[J]. Environment International, 2020, 134:105047 Lima A R A, Barletta M, Costa M F. Seasonal distribution and interactions between plankton and microplastics in a tropical estuary[J]. Estuarine, Coastal and Shelf Science, 2015, 165:213-225 Ding J N, Zhang S S, Razanajatovo R M, et al. Accumulation, tissue distribution, and biochemical effects of polystyrene microplastics in the freshwater fish red tilapia (Oreochromis niloticus)[J]. Environmental Pollution, 2018, 238:1-9 Qiang L Y, Cheng J P. Exposure to microplastics decreases swimming competence in larval zebrafish (Danio rerio)[J]. Ecotoxicology and Environmental Safety, 2019, 176:226-233 Chen Q Q, Lackmann C, Wang W Y, et al. Microplastics lead to hyperactive swimming behaviour in adult zebrafish[J]. Aquatic Toxicology, 2020, 224:105521 Barboza L G A, Lopes C, Oliveira P, et al. Microplastics in wild fish from North East Atlantic Ocean and its potential for causing neurotoxic effects, lipid oxidative damage, and human health risks associated with ingestion exposure[J]. The Science of the Total Environment, 2020, 717:134625 Teuten E L, Saquing J M, Knappe D R U, et al. Transport and release of chemicals from plastics to the environment and to wildlife[J]. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences, 2009, 364(1526):2027-2045 Qiao R X, Deng Y F, Zhang S H, et al. Accumulation of different shapes of microplastics initiates intestinal injury and gut microbiota dysbiosis in the gut of zebrafish[J]. Chemosphere, 2019, 236:124334 Mazurais D, Ernande B, Quazuguel P, et al. Evaluation of the impact of polyethylene microbeads ingestion in European Sea bass (Dicentrarchus labrax) larvae[J]. Marine Environmental Research, 2015, 112(Pt A):78-85 Jabeen K, Li B W, Chen Q Q, et al. Effects of virgin microplastics on goldfish (Carassius auratus)[J]. Chemosphere, 2018, 213:323-332 Wen B, Zhang N, Jin S R, et al. Microplastics have a more profound impact than elevated temperatures on the predatory performance, digestion and energy metabolism of an Amazonian cichlid[J]. Aquatic Toxicology, 2018, 195:67-76 Bhagat J, Zang L Q, Nishimura N, et al. Zebrafish:An emerging model to study microplastic and nanoplastic toxicity[J]. The Science of the Total Environment, 2020, 728:138707 Chen Q Q, Gundlach M, Yang S Y, et al. Quantitative investigation of the mechanisms of microplastics and nanoplastics toward zebrafish larvae locomotor activity[J]. The Science of the Total Environment, 2017, 584-585:1022-1031 Wan Z Q, Wang C Y, Zhou J J, et al. Effects of polystyrene microplastics on the composition of the microbiome and metabolism in larval zebrafish[J]. Chemosphere, 2019, 217:646-658 Han J, Fang Z Q. Estrogenic effects, reproductive impairment and developmental toxicity in ovoviparous swordtail fish (Xiphophorus helleri) exposed to perfluorooctane sulfonate (PFOS)[J]. Aquatic Toxicology, 2010, 99(2):281-290 Wilhelm F D. Fish antioxidant defenses:A comparative approach[J]. Brazilian Journal of Medical and Biological Research, 1996, 29(12):1735-1742 Capó X, Company J J, Alomar C, et al. Long-term exposure to virgin and seawater exposed microplastic enriched-diet causes liver oxidative stress and inflammation in gilthead seabream Sparus aurata, Linnaeus 1758[J]. The Science of the Total Environment, 2021, 767:144976 Zhao Y, Bao Z W, Wan Z Q, et al. Polystyrene microplastic exposure disturbs hepatic glycolipid metabolism at the physiological, biochemical, and transcriptomic levels in adult zebrafish[J]. The Science of the Total Environment, 2020, 710:136279 Wan Z Q, Wang C Y, Zhou J J, et al. Effects of polystyrene microplastics on the composition of the microbiome and metabolism in larval zebrafish[J]. Chemosphere, 2019, 217:646-658 Ory N C, Gallardo C, Lenz M, et al. Capture, swallowing, and egestion of microplastics by a planktivorous juvenile fish[J]. Environmental Pollution, 2018, 240:566-573 Tian J, He G, Mai K S, et al. Effects of postprandial starvation on mRNA expression of endocrine-, amino acid and peptide transporter-, and metabolic enzyme-related genes in zebrafish (Danio rerio)[J]. Fish Physiology and Biochemistry, 2015, 41(3):773-787 姚丹, 吴昊, 江敏. 全氟辛烷磺酸对斑马鱼肝脏影响机制的代谢组学研究[J]. 生态毒理学报, 2018, 13(6):97-106 Yao D, Wu H, Jiang M. Effects of perfluorooctane sulfonate on the liver metabolism of Danio rerio[J]. Asian Journal of Ecotoxicology, 2018, 13(6):97-106(in Chinese)
Tahiliani A G, Beinlich C J. Pantothenic acid in health and disease[J]. Vitamins & Hormones, 1991, 46:165-228 Tabatabaie L, Klomp L W, Berger R, et al. L-serine synthesis in the central nervous system:A review on serine deficiency disorders[J]. Molecular Genetics and Metabolism, 2010, 99(3):256-262 de Koning T J, Snell K, Duran M, et al. L-serine in disease and development[J]. The Biochemical Journal, 2003, 371(Pt 3):653-661 -
点击查看大图
计量
- 文章访问数: 2630
- HTML全文浏览数: 2630
- PDF下载数: 183
- 施引文献: 0
下载:
