MgO NPs及其析出的Mg2+对细叶蜈蚣草(Egeria najas)光合作用的影响
Effects of MgO NPs and Their Released Mg2+ on the Photosynthesis of Egeria najas
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摘要: 以细叶蜈蚣草（Egeria najas）为受试植物，研究MgO NPs及其析出的Mg2+对水生植物光合作用的影响。结果表明，MgO NPs抑制了光系统Ⅱ反应中心之间的连通性及受体侧的电子传递，而Mg2+则提升了光系统活性和光能转化率，因而排除了MgO NPs中析出的Mg2+对细叶蜈蚣草光合作用的毒性。对Mg2+浓度的原位实时检测表明，MgO NPs悬浮液中Mg2+浓度并非常数。在培养细叶蜈蚣草初期，悬浮液中Mg2+析出浓度范围为0.3~1.0 mg·L-1，随时间推移析出浓度逐渐增高并在24 h后达到饱和值0.7~2.4 mg·L-1。未培养细叶蜈蚣草时悬浮液中析出的Mg2+浓度要更大，24 h后浓度范围达到0.9~2.8 mg·L-1。这些结果弥补了以往研究中未能对MgO NPs析出的Mg2+浓度进行动态检测的不足。比较细叶蜈蚣草对MgO NPs悬浮液中和MgCl2溶液中的Mg2+的表观吸收量会发现MgO NPs抑制了细叶蜈蚣草对Mg2+的吸收。Abstract: Egeria najas was used as a test plant to study the effects of MgO NPs and their released Mg2+ on the photosynthesis of aquatic plants. The results showed that MgO NPs inhibited the connectivity between the photosystem Ⅱ (PS Ⅱ) reaction centers and the electron transport on the receptor side. But Mg2+ enhanced the activity of photosystem and the conversion efficiency of light energy, thus eliminated the toxicity of the released Mg2+ on the photosynthesis of Egeria najas. In situ real-time detection of Mg2+ concentration indicated that the Mg2+ concentration in the MgO NPs suspension was not constant. In the early stage of the cultivation of Egeria najas, the concentration of Mg2+ in the suspension ranged within 0.3~1.0 mg·L-1. The concentration increased gradually with time and reached a saturation value of 0.7~2.4 mg·L-1 after 24 h. The saturation concentration of Mg2+ in the suspension without Egeria najas in it was much higher, ranged within 0.9~2.8 mg·L-1 after 24 h. These results made up for the lack of dynamic detection of Mg2+ concentrations in MgO NPs suspensions in previous studies. Comparing the apparent uptake of Mg2+ in MgO NPs suspension and MgCl2 solution showed that MgO NPs inhibited the absorption of Mg2+ by Egeria najas.
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Key words:
- MgO nanoparticle /
- Mg2+ /
- Egeria najas /
- photosynthesis /
- ion selective microelectrode /
- chlorophyll a fluorescence
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