低浓度甲硫醇亚慢性暴露对大鼠肺组织的影响
Effects of Subchronic Exposure to Low Concentration Methylmercaptan on Lung Tissue in Rats
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摘要: 本实验利用低浓度甲硫醇亚慢性暴露模型，研究甲硫醇低剂量慢性暴露的肺毒性效应，为该物质对健康损伤的评价及制订有效防治措施提供参考。大鼠在（1.0±0.2） mg·m-3甲硫醇下暴露30 d、每天暴露6 h，考察了肺组织湿干重比、血清生化指标、肺组织病理及免疫组化指标，分析了暴露对肺组织水通道蛋白（AQP4）和基质金属蛋白酶（MMP-9）的表达影响。研究表明，低浓度甲硫醇亚慢性暴露对大鼠肺组织产生一定损害，湿干重比显著增加，组织损伤程度随暴露时间增加呈现加重趋势，暴露组的肺组织中AQP4表达下调，MMP-9表达上调，提示暴露可能造成肺气肿或者哮喘等呼吸道疾病。Abstract: In this study, the pulmonary toxicity of low-dose chronic exposure to methylmercaptan was studied by using the subchronic exposure model of Sprague-Dawley (SD) rats, which could evaluate toxic effect caused by this substance and propose effective prevention and treatment measures. Rats were exposed in methylmercaptan at (1.0±0.2) mg·m-3 for 30 d and 6 h per day. After exposure, the ratio of wet to dry weight of lung tissue, serum biochemical parameters, pathology of lung tissue and immunohistochemical parameters were studied. The effects of exposure on the expression of aquaporin and matrix metalloproteinase in lung tissue were analyzed. It was found that subchronic exposure to low concentration of methylmercaptan caused adverse effect to the lung tissues of rats, including the significant increase of the wet/dry lung weight ratios. The degree of tissue damage increased with prolonging of the exposure time. The expression of AQP4 of the exposure groups in the lung tissues were down-regulated, while the expression of MMP-9 was up-regulated, suggesting that exposure may cause respiratory diseases such as emphysema or asthma.
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Key words:
- methylmercaptan /
- lung tissue /
- chronic toxicity /
- aquaporin /
- matrix metalloproteinase
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