摘要:
为了探索水体中Cr (VI)对脊尾白虾(Exopalaemon carinicauda)的毒性效应,通过Cr (VI)的暴露(15 d)和清水恢复实验(15 d),研究了Cr (VI)对脊尾白虾肝胰脏的SOD活性、MDA及MTs含量的影响。结果表明,通过15 d的暴露,5 mg·L-1(高浓度)、0.5 mg·L-1(低浓度)实验组肝胰脏的SOD均显著高于对照组水平(p < 0.05),15 d的清水恢复后,两个浓度实验组均能够恢复到对照组水平(p > 0.05)。MDA在暴露阶段呈现持续上升的趋势,且在15 d后两个浓度实验组MDA的含量均显著高于对照组水平(p < 0.05),15 d的清水恢复后,5 mg·L-1实验组的MDA含量依然显著高于对照组水平(p < 0.05),0.5mg·L-1实验组的MDA含量基本恢复到对照组水平(p > 0.05)。通过15 d的暴露,两个浓度实验组MTs的含量均显著高于对照组水平(p < 0.05),15 d的清水恢复后,两个浓度实验组MTs的含量均能够恢复到对照组水平(p > 0.05)。
Abstract:
In order to examine the effect of Cr(VI) exposure on of marine biota, the present study analyzedantioxidant enzyme (SOD) activities, contents of lipid peroxidation levels (LPO) and metallothioneins (MTs) of juvenile rigid-tail prawn Exopalaemon carinicauda in hepatopancreas tissue. The experimental juvenile prawnswerereared at low (0.5 mg·L-1) and high Cr(VI) (5 mg·L-1) concentrations in the first 15 d exposure experiment, respectively. Then, they were put in clean seawater for recovery in the next 15 d. The results show that SOD activities were significantly higher at two exposure groups than at the control group (p < 0.05). By the end of 15 d recovery period, SOD activities in two exposure treatments were similar to that at the control (p > 0.05). The MDA concentration in hepatopancreas tissue at the low and high concentration treatments showed an increasingtrend during the exposure period, and the MDA concentration at two treatment groups were significantly higher than that at the control group (p < 0.05) at the end of 15 d exposure experiment. Through 15 d recovery, MDA concentrations at low concentration treatments showed obviously decreasing and had no differences with that at the control group(p > 0.05). MDA concentrations at the high concentration treatments were still significantly higher than that at the control groups (p < 0.05). The MTs concentrations at two treatment groups were significantly higher than that at the control group(p < 0.05) at the end of 15 d exposure experiment, but MTs concentrations could recover to the value at the control group through 15 d recovery(p > 0.05).
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