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孔涛, 林俊杰, 伏虹旭, 张志玲, 淑敏. 低分子量有机酸对土壤微生物碳源代谢特征的影响[J]. 环境化学, 2017, 36(2): 365-372. doi: 10.7524/j.issn.0254-6108.2016.12.2016042301
引用本文: 孔涛, 林俊杰, 伏虹旭, 张志玲, 淑敏. 低分子量有机酸对土壤微生物碳源代谢特征的影响[J]. 环境化学, 2017, 36(2): 365-372. doi: 10.7524/j.issn.0254-6108.2016.12.2016042301
shu
KONG Tao, LIN Junjie, FU Hongxu, ZHANG Zhiling, SHU Min. Effect of low molecular weight organic acids on metabolic characteristic of soil microbes to carbon sources[J]. Environmental Chemistry, 2017, 36(2): 365-372. doi: 10.7524/j.issn.0254-6108.2016.12.2016042301
Citation: KONG Tao, LIN Junjie, FU Hongxu, ZHANG Zhiling, SHU Min. Effect of low molecular weight organic acids on metabolic characteristic of soil microbes to carbon sources[J]. Environmental Chemistry, 2017, 36(2): 365-372. doi: 10.7524/j.issn.0254-6108.2016.12.2016042301
shu

低分子量有机酸对土壤微生物碳源代谢特征的影响

  • 1.

    辽宁工程技术大学环境科学与工程学院, 阜新, 123000;

  • 2.

    重庆三峡学院三峡库区水环境演变与污染防治重庆高校市级重点实验室, 万州, 404100

  • 基金项目:

    辽宁省自然科学基金（2015020805），国家自然科学基金（41301248），辽宁省科学事业公益研究基金（2015002011），中国科学院水库水环境重点实验室开放基金项目（RAE2014BA01B），教育部春晖计划项目（Z2015133），重庆市教委科学技术研究项目（KJ1601016）资助.

Effect of low molecular weight organic acids on metabolic characteristic of soil microbes to carbon sources

  • 1.

    College of Environmental Science and Engineering, Liaoning Technical University, Fuxin, 123000, China;

  • 2.

    Key Laboratory of Water Environment Evolution and Pollution Control in Three Gorges Reservior, Chongqing Three Georges University, Wanzhou, 404100, China

  • Fund Project: Supported by the Natural Science Foundation of Liaoning Province of China (2015020805), National Natural Science Foundation of China (41301248), Scientific Research Foundation for Public Welfare of Liaoning Province of China (2015002011) and the Key Laboratory of Reservoir Aquatic Environment, Chinese Academy of Science(RAE2014BA01B), Chunhui Project from Education Ministry of China(Z2015133)

  • 摘要: 应用MicroRespTM方法研究了4种低分子量有机酸作用下土壤微生物对不同类型碳源的代谢特征.结果表明，甲酸、草酸、柠檬酸处理下土壤微生物碳源总利用率均高于对照，苯甲酸则与对照没有差异.各有机酸之间进行比较，柠檬酸处理下土壤微生物对碳源总利用率最高，苯甲酸最低；碳源代谢的Shannon指数大小顺序为苯甲酸>柠檬酸≈草酸>甲酸；综合而言，柠檬酸处理下土壤微生物代谢能力最强.4种低分子量有机酸处理下，4大类碳源底物的利用率大小顺序表现为糖类 > 氨基酸类 > 羧酸类≈芳香族类，其中，海藻糖和γ-氨基丁酸的利用率占碳源总利用率的比例最高.主成分分析结果表明，有机酸处理下土壤微生物群落变化的敏感碳源是糖类；各有机酸处理的土壤微生物代谢特征都与对照显著不同，柠檬酸处理下碳源代谢特征与其它3种有机酸明显不同.综上所述，甲酸、草酸、柠檬酸能够提高土壤微生物碳源代谢能力，其中柠檬酸效果最好，可将添加较高浓度的柠檬酸作为提高土壤碳周转率的一种方式.
    Abstract: The carbon source metabolic characteristics of alkaline soil microbial community under 4 types of low molecular weight organic acid treatments was investigated by means of MicroRespTM method. The results showed that formic acid, oxalic acid and citric acid enhanced total carbon utilization rate of soil microbial community in comparison with control, and total utilization of benzoic acid treatment was the not significantly different from that of control treatment. Total carbon source utilization rate of soil microbial community under citric acid treatment was the highest, and total utilization rate under benzoic acid treatment was lowest. Shannon index of carbon sources metabolism followed the order of benzoic acid treatment > citric acid > oxalic acid > formic acid. Conclusively, metabolic function of soil microbe under citric acid treatment was to strongest. Utilization rate of carbon sources substrates followed the order of carbohydrates > amino acids > carboxylic acids > aromatic group. Among 15 carbon sources, the ratio of trehalose and γ-aminobutyric acid utilization rate to total carbon sources utilization rate was the highest. Principle components analysis showed that carbohydrates were the sensitive carbon source of soil microbial community changes. Metabolic characteristics of soil microbial community under 4 types of organic acid treatments were significantly different from that under control treatment. Metabolic characteristics under citric acid treatment was significantly different from that of the other organic acid treatments. In summary, formic acid, oxalic acid and citric acid could enhance carbon source metabolic ability of soil microbial community, and adding high concentration of citric acid to soil could be used as a method of improving soil carbon turnover rate.
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  • 收稿日期:  2016-04-23
  • 刊出日期:  2017-02-15
孔涛, 林俊杰, 伏虹旭, 张志玲, 淑敏. 低分子量有机酸对土壤微生物碳源代谢特征的影响[J]. 环境化学, 2017, 36(2): 365-372. doi: 10.7524/j.issn.0254-6108.2016.12.2016042301
引用本文: 孔涛, 林俊杰, 伏虹旭, 张志玲, 淑敏. 低分子量有机酸对土壤微生物碳源代谢特征的影响[J]. 环境化学, 2017, 36(2): 365-372. doi: 10.7524/j.issn.0254-6108.2016.12.2016042301
shu
KONG Tao, LIN Junjie, FU Hongxu, ZHANG Zhiling, SHU Min. Effect of low molecular weight organic acids on metabolic characteristic of soil microbes to carbon sources[J]. Environmental Chemistry, 2017, 36(2): 365-372. doi: 10.7524/j.issn.0254-6108.2016.12.2016042301
Citation: KONG Tao, LIN Junjie, FU Hongxu, ZHANG Zhiling, SHU Min. Effect of low molecular weight organic acids on metabolic characteristic of soil microbes to carbon sources[J]. Environmental Chemistry, 2017, 36(2): 365-372. doi: 10.7524/j.issn.0254-6108.2016.12.2016042301
shu

低分子量有机酸对土壤微生物碳源代谢特征的影响

  • 1.  辽宁工程技术大学环境科学与工程学院, 阜新, 123000;
  • 2.  重庆三峡学院三峡库区水环境演变与污染防治重庆高校市级重点实验室, 万州, 404100
  • 收稿日期:  2016-04-23
基金项目:

辽宁省自然科学基金（2015020805），国家自然科学基金（41301248），辽宁省科学事业公益研究基金（2015002011），中国科学院水库水环境重点实验室开放基金项目（RAE2014BA01B），教育部春晖计划项目（Z2015133），重庆市教委科学技术研究项目（KJ1601016）资助.

摘要: 应用MicroRespTM方法研究了4种低分子量有机酸作用下土壤微生物对不同类型碳源的代谢特征.结果表明，甲酸、草酸、柠檬酸处理下土壤微生物碳源总利用率均高于对照，苯甲酸则与对照没有差异.各有机酸之间进行比较，柠檬酸处理下土壤微生物对碳源总利用率最高，苯甲酸最低；碳源代谢的Shannon指数大小顺序为苯甲酸>柠檬酸≈草酸>甲酸；综合而言，柠檬酸处理下土壤微生物代谢能力最强.4种低分子量有机酸处理下，4大类碳源底物的利用率大小顺序表现为糖类 > 氨基酸类 > 羧酸类≈芳香族类，其中，海藻糖和γ-氨基丁酸的利用率占碳源总利用率的比例最高.主成分分析结果表明，有机酸处理下土壤微生物群落变化的敏感碳源是糖类；各有机酸处理的土壤微生物代谢特征都与对照显著不同，柠檬酸处理下碳源代谢特征与其它3种有机酸明显不同.综上所述，甲酸、草酸、柠檬酸能够提高土壤微生物碳源代谢能力，其中柠檬酸效果最好，可将添加较高浓度的柠檬酸作为提高土壤碳周转率的一种方式.

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