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氧化锌@石墨烯纳米复合材料的抑菌性能及其细胞毒性

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段慧敏, 杨林松, 周正伟, 何光裕, 王利群, 陈海群. 氧化锌@石墨烯纳米复合材料的抑菌性能及其细胞毒性[J]. 环境化学, 2016, 35(7): 1468-1473. doi: 10.7524/j.issn.0254-6108.2016.07.2015112704
引用本文: 段慧敏, 杨林松, 周正伟, 何光裕, 王利群, 陈海群. 氧化锌@石墨烯纳米复合材料的抑菌性能及其细胞毒性[J]. 环境化学, 2016, 35(7): 1468-1473. doi: 10.7524/j.issn.0254-6108.2016.07.2015112704
shu
DUAN Huimin, YANG Linsong, ZHOU Zhengwei, HE Guangyu, WANG Liqun, CHEN Haiqun. Antibacterial activity and cytotoxicity of ZnO@graphene nanocomposites[J]. Environmental Chemistry, 2016, 35(7): 1468-1473. doi: 10.7524/j.issn.0254-6108.2016.07.2015112704
Citation: DUAN Huimin, YANG Linsong, ZHOU Zhengwei, HE Guangyu, WANG Liqun, CHEN Haiqun. Antibacterial activity and cytotoxicity of ZnO@graphene nanocomposites[J]. Environmental Chemistry, 2016, 35(7): 1468-1473. doi: 10.7524/j.issn.0254-6108.2016.07.2015112704
shu

氧化锌@石墨烯纳米复合材料的抑菌性能及其细胞毒性

  • 1.

    常州大学环境与安全工程学院, 常州市石墨烯环境安全材料重点实验室, 常州, 213164;

  • 2.

    常州大学制药与生命科学学院, 常州, 213164

  • 基金项目:

    国家自然科学基金（51472035，51572036），江苏省科技厅（BE2014089，BY2015027-18），常州市科技局基金（CM20153006）资助.

Antibacterial activity and cytotoxicity of ZnO@graphene nanocomposites

  • 1.

    Changzhou Key Laboratory of Graphene-Based Materials for Environment & Safety, School of Environmental & Safety Engineering, Changzhou University, Changzhou, 213164, China;

  • 2.

    School of Pharmaceuticals and Life Sciences, Changzhou University, Changzhou, 213164, China

  • Fund Project: Supported by the National Natural Science Foundation of China (51472035, 51572036), the Science and Technology Department of Jiangsu Province (BE2014089, BY2015027-18)and Science and Technology Project of Changzhou(CM20153006).

  • 摘要: 以氧化石墨和乙酸锌为原料，通过水热反应成功制备了氧化锌@石墨烯纳米复合材料，采用X射线衍射（XRD）、红外吸收光谱（FTIR）和透射电子显微镜（TEM）对制得的复合材料进行了表征.以大肠杆菌（E.coli）为实验菌种，对复合材料的抑菌性能进行了测试；并选用小鼠成纤维细胞L-929评价了材料的细胞毒性.结果表明，纳米氧化锌颗粒均匀地负载在石墨烯片层上，形貌均一，平均粒径为12 nm左右.复合材料在60 μg·mL-1时可以完全抑制大肠杆菌的生长，是一种效果显著的新型抑菌材料.L-929细胞毒性测试表明复合材料的生物毒性比较缓和，氧化锌@石墨烯纳米复合材料可以作为一种安全高效的无机抑菌材料使用.
    Abstract: ZnO@graphene nanocomposite were prepared by hydrothermal method, using graphite oxide (GO) and zinc acetate as raw materials. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and transmission electron microscope (TEM) were used to characterize the prepared nano-composite. The antibacterial activity of ZnO@graphene nano-composites was tested using E.coli as mode strain. Moreover, cytotoxicity was assessed using L-929 mouse fibroblast cells. The results of characterization indicated that zinc oxide nanoparticles were uniformly dispersed on graphene surface with particle size around 12 nm. The minimal inhibitory concentration of ZnO@graphene against E.coli was 60 μg·mL-1, suggesting that the composite was an outstanding antibacterial material. Additionally, the toxicity tests demorstrated that the prepared nano-composite posed no significant toxicity on L-929 cells. Therefore, ZnO@graphene nano-composites can be used as a new type of antibacterial material.
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  • 收稿日期:  2015-11-27
  • 刊出日期:  2016-07-15
段慧敏, 杨林松, 周正伟, 何光裕, 王利群, 陈海群. 氧化锌@石墨烯纳米复合材料的抑菌性能及其细胞毒性[J]. 环境化学, 2016, 35(7): 1468-1473. doi: 10.7524/j.issn.0254-6108.2016.07.2015112704
引用本文: 段慧敏, 杨林松, 周正伟, 何光裕, 王利群, 陈海群. 氧化锌@石墨烯纳米复合材料的抑菌性能及其细胞毒性[J]. 环境化学, 2016, 35(7): 1468-1473. doi: 10.7524/j.issn.0254-6108.2016.07.2015112704
shu
DUAN Huimin, YANG Linsong, ZHOU Zhengwei, HE Guangyu, WANG Liqun, CHEN Haiqun. Antibacterial activity and cytotoxicity of ZnO@graphene nanocomposites[J]. Environmental Chemistry, 2016, 35(7): 1468-1473. doi: 10.7524/j.issn.0254-6108.2016.07.2015112704
Citation: DUAN Huimin, YANG Linsong, ZHOU Zhengwei, HE Guangyu, WANG Liqun, CHEN Haiqun. Antibacterial activity and cytotoxicity of ZnO@graphene nanocomposites[J]. Environmental Chemistry, 2016, 35(7): 1468-1473. doi: 10.7524/j.issn.0254-6108.2016.07.2015112704
shu

氧化锌@石墨烯纳米复合材料的抑菌性能及其细胞毒性

  • 1.  常州大学环境与安全工程学院, 常州市石墨烯环境安全材料重点实验室, 常州, 213164;
  • 2.  常州大学制药与生命科学学院, 常州, 213164
  • 收稿日期:  2015-11-27
基金项目:

国家自然科学基金（51472035，51572036），江苏省科技厅（BE2014089，BY2015027-18），常州市科技局基金（CM20153006）资助.

摘要: 以氧化石墨和乙酸锌为原料，通过水热反应成功制备了氧化锌@石墨烯纳米复合材料，采用X射线衍射（XRD）、红外吸收光谱（FTIR）和透射电子显微镜（TEM）对制得的复合材料进行了表征.以大肠杆菌（E.coli）为实验菌种，对复合材料的抑菌性能进行了测试；并选用小鼠成纤维细胞L-929评价了材料的细胞毒性.结果表明，纳米氧化锌颗粒均匀地负载在石墨烯片层上，形貌均一，平均粒径为12 nm左右.复合材料在60 μg·mL-1时可以完全抑制大肠杆菌的生长，是一种效果显著的新型抑菌材料.L-929细胞毒性测试表明复合材料的生物毒性比较缓和，氧化锌@石墨烯纳米复合材料可以作为一种安全高效的无机抑菌材料使用.

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