抗坏血酸对铜胁迫下广藿香幼苗生长、铜积累和抗氧化酶活性的影响
Effects of exogenous ascorbic acid on seedling growth, copper accumulation and antioxidant enzyme activities of Pogostemon cablin under copper stress
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摘要: 通过室内水培试验，研究了外源添加抗坏血酸（Ascorbic acid，AsA）对铜（Copper， Cu）胁迫下广藿香（Pogostemon cablin （Blanco） Benth.）幼苗生长、Cu2+积累和抗氧化酶活性的影响.结果显示，与对照相比，10-80 μmol·L-1 Cu2+降低了广藿香幼苗的苗长、根长、植株生物量和根系耐性指数，且随Cu2+浓度增大抑制作用增强.与Cu2+胁迫相比，外源添加0.10-0.50 mmol·L-1 AsA提高了广藿香幼苗的苗长、根长、植株生物量、根系耐性指数、根系活力以及SOD、POD、CAT和APX酶活性，降低其根系相对电导率、MDA含量和Cu2+含量；随AsA浓度的增加，苗长、根长、植株生物量、根系耐性指数、根系活力以及POD和CAT活性先升高后下降，相对电导率、MDA含量和Cu2+含量先下降后上升，SOD和APX活性则一直增加.结果显示，外源添加0.25 mmol·L-1的AsA能提高广藿香幼苗细胞清除活性氧的能力，一定程度上缓解40 μmol·L-1 Cu2+胁迫对膜系统的伤害，减轻Cu2+胁迫对广藿香幼苗生长的毒害作用.Abstract: Effects of exogenous ascorbic acid on the growth, copper accumulation and antioxidant enzyme activities of Pogostemon cablin (Blanco) Benth. seedlings under copper stress were studied by a hydroponic experiment in the room. The results showed that, compared with control, shoot length, root length, biomass and root tolerance index were all reduced by 10-80 μmol·L-1 Cu2+. With the increase of Cu2+ concentration, the inhibitory effects were enhanced. Compared with copper stress, shoot length, root length, biomass, root tolerance index, root vigor and the activities of superoxide dismutase, peroxidase, catalase and aseorbateperoxidase in roots of pogostemon cablin seedling were all improved by exogenous ascorbic acid with 0.10-0.50 mmol·L-1, while relative electrical conductivity, copper accumulation and malondialdehyde content were reduced. With the increase of AsA concentration, shoot length, root length, biomass, root tolerance index, root vigor and the activities of peroxidase and catalase first increased and then decreased. Relative electrical conductivity, copper accumulation and malondialdehyde content first decreased and then increased. The activities of superoxide dismutase and aseorbateperoxidase increased. The above results indicated that the ability of eliminating reactive oxygen species could be improved and the damage of cell membrane system by 40 μmol·L-1 Cu+2could be alleviated to some degree by exogenous 0.25 mmol·L-1 ascorbic acid.
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Key words:
- Pogostemon cablin (Blanco) Benth. /
- copper stress /
- ascorbic acid /
- seedling growth /
- antioxidant enzyme
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