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六溴环十二烷(HBCD)对人肝癌细胞HepG2的细胞毒性及机制

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向明钧, 陈玲, 李宇丹, 张钰华, 周佩云, 刘铁军, 孙远鹏, 伍梦娟, 方星薪, 杨超萍. 六溴环十二烷(HBCD)对人肝癌细胞HepG2的细胞毒性及机制[J]. 环境化学, 2016, 35(2): 311-316. doi: 10.7524/j.issn.0254-6108.2016.02.2015081003
引用本文: 向明钧, 陈玲, 李宇丹, 张钰华, 周佩云, 刘铁军, 孙远鹏, 伍梦娟, 方星薪, 杨超萍. 六溴环十二烷(HBCD)对人肝癌细胞HepG2的细胞毒性及机制[J]. 环境化学, 2016, 35(2): 311-316. doi: 10.7524/j.issn.0254-6108.2016.02.2015081003
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XIANG Mingjun, CHEN Ling, LI Yudan, ZHANG Yuhua, ZHOU Peiyun, LIU Tiejun, SUN Yuanpeng, WU Mengjuan, FANG Xingxin, YANG Chaoping. The cytotoxicity and mechanism of Hexabromoeyelododecane(HBCD) on HepG2 cells[J]. Environmental Chemistry, 2016, 35(2): 311-316. doi: 10.7524/j.issn.0254-6108.2016.02.2015081003
Citation: XIANG Mingjun, CHEN Ling, LI Yudan, ZHANG Yuhua, ZHOU Peiyun, LIU Tiejun, SUN Yuanpeng, WU Mengjuan, FANG Xingxin, YANG Chaoping. The cytotoxicity and mechanism of Hexabromoeyelododecane(HBCD) on HepG2 cells[J]. Environmental Chemistry, 2016, 35(2): 311-316. doi: 10.7524/j.issn.0254-6108.2016.02.2015081003
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六溴环十二烷(HBCD)对人肝癌细胞HepG2的细胞毒性及机制

  • 1.

    吉首大学医学院;

  • 2.

    吉首大学生物资源与环境科学学院, 吉首, 416000

  • 基金项目:

    吉首大学2013年大学生研究性学习与创新性实验计划项目(JSU-CX-2013-32)

    国家自然科学基金(81360397)资助.

The cytotoxicity and mechanism of Hexabromoeyelododecane(HBCD) on HepG2 cells

  • 1.

    College of Medical Science;

  • 2.

    College of Biology and Environmental Sciences, Jishou University, Jishou, 416000, China

  • Fund Project: Supported by the Students' Research Learning and Innovative Experimental Project of Jishou University(JSU-CX-2013-32) and the National Natural Science Foundation of China(81360397).

  • 摘要: 为研究六溴环十二烷(Hexabromoeyelododecane, HBCD)对人肝癌细胞HepG2的毒性效应及机制,设3个不同剂量HBCD试验组(10、15、20μg·mL-1)和溶剂对照组、阴性对照组及阳性对照组,将HepG2细胞处理24、48、72 h后,用MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide)法检测细胞毒性;24 h后采用胞质分裂阻断法测定微核率;另在24 h后用细胞内2,7-二氢二氯荧光素(2',7'-dichlorofluorescin diacetate, DCFH-DA)法测定细胞内活性氧(Reactive oxygen species, ROS)的浓度.实验显示,HBCD对HepG2细胞有毒性效应,且存在一定的时间和剂量效应,并导致HepG2细胞的微核率和活性氧的浓度增高.研究表明,HBCD对HepG2细胞有明显的细胞毒性.
    Abstract: For understanding the mechanism and the cytotoxicity of HBCD(Hexabromoeyelododecane)on HepG2 cells, we used the MTT assay,a cytokinesis-block micronucleus assay to evaluate the cytotoxicity of HBCD(10,15,20μg·mL-1) on HepG2 cells as well as the control groups, and measured the fluorescent intensity of reactive oxygen species(ROS) using DCFH-DA. The results showed that the higher concentrations of HBCD reduced the cell survival rates. There were significant differences in the frequency of cytokinesis-block micronucleus between the groups. A significant increased frequency of cytokinesis-block micronucleus was observed in 10,15,20μg·mL-1 dose group compared with the negative control group(PPPin vitro.
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  • 收稿日期:  2015-08-10
  • 刊出日期:  2016-02-15

六溴环十二烷(HBCD)对人肝癌细胞HepG2的细胞毒性及机制

  • 1.  吉首大学医学院;
  • 2.  吉首大学生物资源与环境科学学院, 吉首, 416000
  • 收稿日期:  2015-08-10
基金项目:

吉首大学2013年大学生研究性学习与创新性实验计划项目(JSU-CX-2013-32)

国家自然科学基金(81360397)资助.

摘要: 为研究六溴环十二烷(Hexabromoeyelododecane, HBCD)对人肝癌细胞HepG2的毒性效应及机制,设3个不同剂量HBCD试验组(10、15、20μg·mL-1)和溶剂对照组、阴性对照组及阳性对照组,将HepG2细胞处理24、48、72 h后,用MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide)法检测细胞毒性;24 h后采用胞质分裂阻断法测定微核率;另在24 h后用细胞内2,7-二氢二氯荧光素(2',7'-dichlorofluorescin diacetate, DCFH-DA)法测定细胞内活性氧(Reactive oxygen species, ROS)的浓度.实验显示,HBCD对HepG2细胞有毒性效应,且存在一定的时间和剂量效应,并导致HepG2细胞的微核率和活性氧的浓度增高.研究表明,HBCD对HepG2细胞有明显的细胞毒性.

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