藻毒素β-N-甲氨基-L-丙氨酸(BMAA)的氯化降解
Chlorination degradation of neurotoxin β-N-methylamino-L-alanine
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摘要: 本文建立了高效液相色谱-串联质谱法(HPLC-MS/MS)来检测水中BMAA,并以次氯酸钠为消毒剂进行模拟氯化,研究BMAA的氯化降解及环境因子对BMAA降解的影响.结果表明,采用亲水交换作用色谱柱建立的HPLC-MS/MS分析方法,样品前处理过程简单易操作,在2-500μg·L-1范围内线性关系良好(R2=0.9995),且检出限低(0.2μg·L-1).在常规条件下(Cl:N=2:1,T=20℃,pH=7),BMAA能通过氯化得到有效降解,2 h降解率达到80%,6 h降解率达到97%;低的Cl:N,低温及碱性条件下,BMAA的降解率显著降低.研究结果为饮用水预氯化及氯化消毒去除BMAA提供了理论依据.
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关键词:
- β-N-甲氨基-L-丙氨酸(BMAA) /
- 高效液相色谱-质谱 /
- 模拟氯化 /
- 降解
Abstract: β-N-methyl amino-L-alanine(BMAA), a neurotoxin produced by cyanobacteria, has been detected in surface water containing algae. This study established a detection method of BMAA in water sample through High Performance Liquid Chromatography-Tandem Mass Spectrometry(HPLC-MS/MS) and investigated the degradation of BMAA by simulated chlorination using sodium hypochlorite. The results illustrated that BMAA could be detected by HPLC-MS/MS with a hydrophilic interaction liquid chromatography column. A simple pre-treatment method was also developed. A good linear relationship was obtained(R2=0.9995) while the concentration of BMAA ranged from 2 to 500μg·L-1, and a low detection limit(0.2μg·L-1) was achieved. Under the general condition(Cl/N=2:1, T=20℃, pH=7), the degradation rate of BMAA was high and was more than 80% in 2 h and about 97% in 6 h. However, compared to the general condition, the degradation rate of BMAA decreased significantly under the conditions of lower Cl/N(0.25), lower temperature(10℃) and higher alkaline. These results could provide a basis for the removal of BMAA in the pre-chlorination and chlorination disinfection of drinking water. -
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