高级搜索

纳米金颗粒进入梨形四膜虫体内的方式及其分布

,  , 

downloadPDF

上一篇

下一篇

徐斌, 史俊朋, 张洪武. 纳米金颗粒进入梨形四膜虫体内的方式及其分布[J]. 环境化学, 2012, 31(11): 1803-1807.
引用本文: 徐斌, 史俊朋, 张洪武. 纳米金颗粒进入梨形四膜虫体内的方式及其分布[J]. 环境化学, 2012, 31(11): 1803-1807.
shu
XU Bin, SHI Junpeng, ZHANG Hongwu. The uptake and distribution of gold nanoparticles in tetrahymena pyriformis[J]. Environmental Chemistry, 2012, 31(11): 1803-1807.
Citation: XU Bin, SHI Junpeng, ZHANG Hongwu. The uptake and distribution of gold nanoparticles in tetrahymena pyriformis[J]. Environmental Chemistry, 2012, 31(11): 1803-1807.
shu

纳米金颗粒进入梨形四膜虫体内的方式及其分布

  • 1.

    中国科学院城市环境研究所, 厦门, 361021

  • 基金项目:

    厦门市科技项目(3502Z20101010)

    973前期(2010CB434802)资助.

The uptake and distribution of gold nanoparticles in tetrahymena pyriformis

  • 1.

    Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China

  • Fund Project:

  • 摘要: 以梨形四膜虫为模式动物,研究水体中的纳米金颗粒进入生物体的方式、分布及其生物学效应.实验中所采用的纳米金颗粒平均粒径为13 nm,分散均匀.透射电镜结果表明,纳米金颗粒可以通过口沟(进食)和细胞膜(内吞)两种方式进入梨形四膜虫体内;纳米金颗粒进入梨形四膜虫体内后,主要分布在食物泡中,另外细胞质和细胞核(大核)中也含有少量的纳米金颗粒.同时,研究表明在实验所用的浓度范围内,纳米金颗粒对梨形四膜虫无明显毒性.
    Abstract: Tetrahymena pyriformis was chosen as the model organism to investigate the uptake and distribution of gold nanoparticles in aquatic animal. Gold nanoparticles with an average size of 13 nm were used in this experiment. The results showed that ingestion and endocytosis were the two most important processes for gold nanoparticle uptake by Tetrahymena pyriformis. Gold nanoparticles distributed in food vacuole, cytoplasm and macronuclear of Tetrahymena pyriformis. Statistical results showed that there was no obvious toxicity to Tetrahymena pyriformis with the exposure concentrations of gold nanoparticles used in the experiment.
  • 加载中
  • [1] Buzea C, Pacheco I I, Robbie K. Nanomaterials and nanoparticles: Sources and toxicity [J]. Biointerphases, 2007, 2(4):1934-8630
    [2] Limbach L K, Wick P, Manser P, et al. Exposure of engineered nanoparticles to human lung epithelial cells: Influence of chemical composition and catalytic activity on oxidative stress [J]. Environ Sci Technol, 2007, 41:4158-4163
    [3] 李晶,胡霞林,陈启晴,等. 纳米材料对水生生物的生态毒理效应研究进展 [J]. 环境化学,2011,30(12):1993-2002
    [4] Service R F. Nanomaterials show signs of toxicity [J]. Science, 2003, 300 (5617):243
    [5] 贾俊彩,王锋,余济美. 斑马鱼在纳米金属及金属氧化物毒性研究中的应用 [J]. 环境化学,2011, 30(1):153-157
    [6] Wiesner M R, Lowry G V, Alvarez P, et al. Assessing the risks of manufactured nanomaterials [J]. Environ Sci Technol, 2006, 40(14):4336-4345
    [7] Rosi N L, Mirkin C A. Nanostructures in Biodiagnostics [J]. Chem Rev, 2005, 105(4):1547-1562
    [8] Tominaga J, Michalcea C, Buchel D, et al. Local plasmon photonic transistor [J]. Appl Phys Lett, 2001, 78(17):2417-2419
    [9] Xiong Y J, Wiley B J, Xia Y N. Nanocrystals with conventional shaped-A class of promising catalysis [J]. Angew Chem Int Edit, 2007, 46(38):7157-7159
    [10] Kuo T R, Hovhannisyan A, Chao Y C, et al. Multiple release kinetics of targeted drug from gold nanorod embedded poly-electrolyte conjugates induced by near-infrared laser irradiation [J]. J Am Chem Soc, 2010, 132(40):14163-14171
    [11] 姚晶,林燕,李佳,等. 三种纳米金颗粒对聚合酶链式反应扩增效率和特异性的影响[J]. 环境化学, 2012, 31(1):1-8
    [12] Priyabrata M, Resham B, Debabrata M. Gold nanoparticles bearing functional anti-cancer drug and anti-aniogenic agent: A "2 in 1" system with potential application in cancer therapeutics [J]. J Biomed Nanotechnol, 2005,1(2): 224-228
    [13] Wang H F, Huff T B, Zweifel D A, et al. In vitro and in vivo two-photo luminescence imaging of single gold nanorod [J]. P Natl Acade Sci USA, 2005, 102:15752-15756
    [14] Bilberg K, Malte H, Wang T, et al. Silver nanoparticles and silver nitrate cause respiratory stress in Eurasian Perch (Perca fluviatilis) [J]. Aquat Toxicol, 2010, 96(2):159-165
    [15] Frankel J. Cell biology of Tetrahymena thermophila[M]. Methods in Cell Biology, 1999, 62:27-125
    [16] Andrea G, Ana M G, Ruth O, et al. Flow cytometry assessment of cytotoxicity and reactive oxygen species generation by single and binary mixtures of cadmium, zinc and copper on populations of the ciliated protozoan Tetrahymena thermophila[J]. Chemosphere, 2007, 68:647-661
    [17] Porter A E, Gass M, Muller K, et al. Direct imaging of single-walled carbon nanotubes in cells [J]. Nat nanotechnol, 2007, 12:713-717
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  1099
  • HTML全文浏览数:  1069
  • PDF下载数:  408
  • 施引文献:  0
出版历程
  • 收稿日期:  2011-12-31
徐斌, 史俊朋, 张洪武. 纳米金颗粒进入梨形四膜虫体内的方式及其分布[J]. 环境化学, 2012, 31(11): 1803-1807.
引用本文: 徐斌, 史俊朋, 张洪武. 纳米金颗粒进入梨形四膜虫体内的方式及其分布[J]. 环境化学, 2012, 31(11): 1803-1807.
shu
XU Bin, SHI Junpeng, ZHANG Hongwu. The uptake and distribution of gold nanoparticles in tetrahymena pyriformis[J]. Environmental Chemistry, 2012, 31(11): 1803-1807.
Citation: XU Bin, SHI Junpeng, ZHANG Hongwu. The uptake and distribution of gold nanoparticles in tetrahymena pyriformis[J]. Environmental Chemistry, 2012, 31(11): 1803-1807.
shu

纳米金颗粒进入梨形四膜虫体内的方式及其分布

  • 1. 中国科学院城市环境研究所, 厦门, 361021
  • 收稿日期:  2011-12-31
基金项目:

厦门市科技项目(3502Z20101010)

973前期(2010CB434802)资助.

摘要: 以梨形四膜虫为模式动物,研究水体中的纳米金颗粒进入生物体的方式、分布及其生物学效应.实验中所采用的纳米金颗粒平均粒径为13 nm,分散均匀.透射电镜结果表明,纳米金颗粒可以通过口沟(进食)和细胞膜(内吞)两种方式进入梨形四膜虫体内;纳米金颗粒进入梨形四膜虫体内后,主要分布在食物泡中,另外细胞质和细胞核(大核)中也含有少量的纳米金颗粒.同时,研究表明在实验所用的浓度范围内,纳米金颗粒对梨形四膜虫无明显毒性.

English Abstract

    全文HTML

参考文献 (17)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心