西洋参中六种农药残留量的HPLC分析
RP-HPLC DETERMINATION OF RESIDUE OF 6 PESTICIDES IN PANAX QUINQUEFOLIUM
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摘要: 本文报道了甲基对硫磷等六种农药在西洋参中多残留的高效液相色谱检测方法.采用乙醚超声波提取,经硅胶柱净化,紫外检测波长采用程序控制,在检测的时间内自动进行四次改变,使每一被检测的农药均在其最大紫外吸收波长处被检测.波长变化为270um 16.0min 230nm 7.5min 205nm 11.5min 210nm.流动相为甲醇-水-乙腈(30:27.5:42.5V/V).当添加水平在1.0-3.0μg·g-1时,回收率大多在80%以上.Abstract: A HPLC method for determination of the residues of 6 pesticides such as Parathion-methyl, Parathion, Endrin, Fenpropathrin, DDT and Aldrin in panax quinquefolium L was described, the mixture was extracted with ether, cleaned up on a silica column and determined in different wavelength with program exchanging. The mobile phase was a mixture of methanol-water-acetonitrine (30:27.5:42.5,V/V). Recoveries at 3 levels of fortification were greater than 80% for most pesticides.
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Key words:
- HPLC /
- pesticide residue /
- Panax quinquefolium
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