广玉兰叶片浸提液中抑铜绿微囊藻化学成分分析

董昆明; 缪莉; 李楠; 周晓见; 靳翠丽; 封克

环境化学

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董昆明, 缪莉, 李楠, 周晓见, 靳翠丽, 封克. 广玉兰叶片浸提液中抑铜绿微囊藻化学成分分析[J]. 环境化学, 2011, 30(7): 1253-1258.

引用本文:

董昆明, 缪莉, 李楠, 周晓见, 靳翠丽, 封克. 广玉兰叶片浸提液中抑铜绿微囊藻化学成分分析[J]. 环境化学, 2011, 30(7): 1253-1258.

DONG Kunming, MIAO Li, LI Nan, ZHOU Xiaojian, JIN Cuili, FENG Ke. ANALYSIS OF THE INHIBITORY COMPONENTS FROM EXTRACTS OF MAGNOLIA GRANDIFLORA LEAVES AGAINST MICROCYSTIS AERUGINOSA[J]. Environmental Chemistry, 2011, 30(7): 1253-1258.

Citation:

广玉兰叶片浸提液中抑铜绿微囊藻化学成分分析

1.
扬州大学环境科学与工程学院, 扬州, 225127;
2.
扬州大学海洋科学与技术研究所, 扬州, 225127;
3.
江苏扬州农业环境安全服务中心, 扬州, 225127
基金项目:
国家自然科学基金项目资助(30671240,30871588,41006097)

江苏省环境材料与环境工程重点实验室开放基金(K090025).

ANALYSIS OF THE INHIBITORY COMPONENTS FROM EXTRACTS OF MAGNOLIA GRANDIFLORA LEAVES AGAINST MICROCYSTIS AERUGINOSA

1.
School of Environmental Science and Engineering, Yang Zhou University, Yangzhou, 225127, China;
2.
Institute of Marine Science and Technology, Yang Zhou University, Yangzhou, 225127, China;
3.
Yangzhou Technological Service Center for Agro-Environment Safety of Jiangsu Province, Yangzhou, 225127, China
Fund Project:

摘要: 针对水华的主要物种——铜绿微囊藻,测定近30种常用绿化植物的浸取液对其增殖的抑制作用,并对其中表现最优的广玉兰样品进行了抑制效果的进一步测定.结果显示,广玉兰叶片浸取物在浓度8 g·L-1的条件下对铜绿微囊藻生长的抑制效果最高可达97.4%.在活性测试的基础上利用硅胶柱层析、凝胶柱层析、GC-MS等方法,结合抑藻实验对广玉兰叶片提取物进行了铜绿微囊藻抑制活性物质的分离纯化和结构鉴定.从广玉兰叶片的高活性提取物正丁醇浸膏中分离出2-亚甲基-3-羟基胆甾烷、正三十七醇、25,26-二羟基维生素D3等含羟基物质,7-甲基-3-亚甲基-6-(3-丁酮基)-3,3a,4,7,8,8a-六氢化-2H-环庚三烯并呋喃-2-酮、6--4,8a-二甲基-4a,5,6,7,8,8a-六氢化-2(1H)-萘酮等酮类物质,以及邻苯二甲酸单(2-乙基)己酯等.
- 广玉兰 /
- 铜绿微囊藻 /
- 抑藻
Abstract: The organic extracts of some terrestrial plant species were screened for their antialgal activity against Microcystis aeruginose.Among the 30 plant species,the extracts of Magnolia grandflora leaves showed the most remarkable activity(97.4%).Silica gel and Sephadex LH20 column chromatography were used to isolate and purify the active substances from the M.grandflora leave extracts.Antialgal assays were used to guide the purification.After that,the compounds in the most active sub-fractions were identified by using GC-MS.Large amounts of alcohols(such as 2-methyl-3-hydroxy bile sterane,1-heptatriacotanol and 25,26-dihydroxy-vitamin D3),ketones(such as 7-methyl-3-methylene-6-(3-methyl ethyl ketene)-3,3a,4,7,8,8a-haxahdro-2H-cyclohepta triene and[b]furan-2-one,6-[1-(hydroxymethyl)-vinyl]-4,8a-dimethyl-4a,5,6,7,8,8a-hexahydro-2(1H)-naphthyl ketone) and lipids(such as mono(2-ethylhexyl)-phthaiate) were identified.The results indicated that these high-content alcohols,ketones and lipids might be the anti-algal substances,seperate or synergistic,in the M.grandflora leaves.This study identified the potential antialgal substances from the M.grandflora.
- Magnolia grandflora /
- Microcystis aeruginosa /
- antialgal

[1]	Zhang W H, Zhang X H, Zhang G M. Variation of Microcystions in a lake for water supply[J]. J Environ Sci Health (part A), 2003, 38(12):2857-2865
[2]	Lu Y,Wen J F. Isolation, pure cultivation and total DNA extraction of Microcystis aeruginosa Kutz in Dianchi Lake[J]. J Lake Sci, 2001, 13(3):285-288
[3]	陈宇炜,高锡云.太湖微囊藻的生长特征及其分离提纯培养的初步研究[J]. 湖泊科学, 1999,11(4):351-356
[4]	Della G M, Isidori M, Lavorgna M et al. Bioactivity of phenanthrenes from Juncus acutus on Selenastrum capricornutum[J]. J Chem Ecol, 2004, 30(4):867-879
[5]	庄源益, 赵凡, 戴树桂, 等. 高等水生植物对藻类生长的克制效应[J]. 环境科学进展,1995,6(3):44-49
[6]	Nakai S, Inoue Y, Hosomi M, et al. Growth inhibition of blue-green algae by allelopathic effects of marophytes[J].Wat Sci Tech,1999, 39(8):47-53
[7]	花铭,陈良燕,尹大强.邻苯三酚和咖啡酸对铜绿微囊藻的化感作用及其机理[J]. 环境化学,2008,27(3):331-334
[8]	Jones G J, Orr P T. Release and degradation of microcystin following algicide treatment of a Microcystis aeruginosa bloom in a recreational lake, as determined by HPLC and protein phosphatase inhibition assay[J].Water Res, 1994, 28(4):871-876
[9]	张维昊,周连凤,吴小刚,等. 菖蒲对铜绿微囊藻的化感作用[J].中国环境科学,2006,26(3):355-358
[10]	洪喻,胡洪营,黄晶晶,等.芦竹抑藻物质的初步分离及抑制铜绿微囊藻的效果[J].环境化学,2008,27(6):751-755
[11]	Li F M, Hu H Y. Isolation and Characterization of a novel antialgal allelochemical from Phragmites communis[J]. App Environ Microbiol, 2005,71(12):6545-6553
[12]	胡正君,史亚利,蔡亚岐. 气相色谱-质谱法测定人体血样中合成麝香[J].环境化学,2010,29(3):530-535

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董昆明, 缪莉, 李楠, 周晓见, 靳翠丽, 封克. 广玉兰叶片浸提液中抑铜绿微囊藻化学成分分析[J]. 环境化学, 2011, 30(7): 1253-1258.

引用本文:

董昆明, 缪莉, 李楠, 周晓见, 靳翠丽, 封克. 广玉兰叶片浸提液中抑铜绿微囊藻化学成分分析[J]. 环境化学, 2011, 30(7): 1253-1258.

Citation:

广玉兰叶片浸提液中抑铜绿微囊藻化学成分分析

1. 扬州大学环境科学与工程学院, 扬州, 225127;
2. 扬州大学海洋科学与技术研究所, 扬州, 225127;
3. 江苏扬州农业环境安全服务中心, 扬州, 225127

收稿日期: 2010-08-24

基金项目:

国家自然科学基金项目资助(30671240,30871588,41006097)

江苏省环境材料与环境工程重点实验室开放基金(K090025).

关键词:

广玉兰 /
铜绿微囊藻 /
抑藻

摘要: 针对水华的主要物种——铜绿微囊藻,测定近30种常用绿化植物的浸取液对其增殖的抑制作用,并对其中表现最优的广玉兰样品进行了抑制效果的进一步测定.结果显示,广玉兰叶片浸取物在浓度8 g·L-1的条件下对铜绿微囊藻生长的抑制效果最高可达97.4%.在活性测试的基础上利用硅胶柱层析、凝胶柱层析、GC-MS等方法,结合抑藻实验对广玉兰叶片提取物进行了铜绿微囊藻抑制活性物质的分离纯化和结构鉴定.从广玉兰叶片的高活性提取物正丁醇浸膏中分离出2-亚甲基-3-羟基胆甾烷、正三十七醇、25,26-二羟基维生素D3等含羟基物质,7-甲基-3-亚甲基-6-(3-丁酮基)-3,3a,4,7,8,8a-六氢化-2H-环庚三烯并呋喃-2-酮、6--4,8a-二甲基-4a,5,6,7,8,8a-六氢化-2(1H)-萘酮等酮类物质,以及邻苯二甲酸单(2-乙基)己酯等.

English Abstract

ANALYSIS OF THE INHIBITORY COMPONENTS FROM EXTRACTS OF MAGNOLIA GRANDIFLORA LEAVES AGAINST MICROCYSTIS AERUGINOSA

1. School of Environmental Science and Engineering, Yang Zhou University, Yangzhou, 225127, China;
2. Institute of Marine Science and Technology, Yang Zhou University, Yangzhou, 225127, China;
3. Yangzhou Technological Service Center for Agro-Environment Safety of Jiangsu Province, Yangzhou, 225127, China

Received Date: 2010-08-24

Fund Project:

Keywords:

Abstract: The organic extracts of some terrestrial plant species were screened for their antialgal activity against Microcystis aeruginose.Among the 30 plant species,the extracts of Magnolia grandflora leaves showed the most remarkable activity(97.4%).Silica gel and Sephadex LH20 column chromatography were used to isolate and purify the active substances from the M.grandflora leave extracts.Antialgal assays were used to guide the purification.After that,the compounds in the most active sub-fractions were identified by using GC-MS.Large amounts of alcohols(such as 2-methyl-3-hydroxy bile sterane,1-heptatriacotanol and 25,26-dihydroxy-vitamin D3),ketones(such as 7-methyl-3-methylene-6-(3-methyl ethyl ketene)-3,3a,4,7,8,8a-haxahdro-2H-cyclohepta triene and[b]furan-2-one,6-[1-(hydroxymethyl)-vinyl]-4,8a-dimethyl-4a,5,6,7,8,8a-hexahydro-2(1H)-naphthyl ketone) and lipids(such as mono(2-ethylhexyl)-phthaiate) were identified.The results indicated that these high-content alcohols,ketones and lipids might be the anti-algal substances,seperate or synergistic,in the M.grandflora leaves.This study identified the potential antialgal substances from the M.grandflora.

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广玉兰叶片浸提液中抑铜绿微囊藻化学成分分析

ANALYSIS OF THE INHIBITORY COMPONENTS FROM EXTRACTS OF MAGNOLIA GRANDIFLORA LEAVES AGAINST MICROCYSTIS AERUGINOSA

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广玉兰叶片浸提液中抑铜绿微囊藻化学成分分析

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ANALYSIS OF THE INHIBITORY COMPONENTS FROM EXTRACTS OF MAGNOLIA GRANDIFLORA LEAVES AGAINST MICROCYSTIS AERUGINOSA

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