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一株同时降解3种邻苯二甲酸酯降解菌的筛选鉴定及降解特性

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梁浩花, 王亚娟, 陶红, 张小红. 一株同时降解3种邻苯二甲酸酯降解菌的筛选鉴定及降解特性[J]. 环境化学, 2019, (12): 2808-2818. doi: 10.7524/j.issn.0254-6108.2019010406
引用本文: 梁浩花, 王亚娟, 陶红, 张小红.

一株同时降解3种邻苯二甲酸酯降解菌的筛选鉴定及降解特性

[J]. 环境化学, 2019, (12): 2808-2818. doi: 10.7524/j.issn.0254-6108.2019010406
shu
LIANG Haohua, WANG Yajuan, TAO Hong, ZHANG Xiaohong. Isolation, identification and degradation characteristics of a three phthalates degrading bacterium[J]. Environmental Chemistry, 2019, (12): 2808-2818. doi: 10.7524/j.issn.0254-6108.2019010406
Citation: LIANG Haohua, WANG Yajuan, TAO Hong, ZHANG Xiaohong.

Isolation, identification and degradation characteristics of a three phthalates degrading bacterium

[J]. Environmental Chemistry, 2019, (12): 2808-2818. doi: 10.7524/j.issn.0254-6108.2019010406
shu

一株同时降解3种邻苯二甲酸酯降解菌的筛选鉴定及降解特性

  • 1. 宁夏大学资源环境学院, 银川, 750021;

  • 2. 宁夏大学经济管理学院, 银川, 750021;

  • 3. 宁夏(中阿)旱区资源评价与环境调控重点实验室, 银川, 750021

    • 通讯作者: 王亚娟, E-mail: 358437013@qq.com ; 
  • 基金项目:

    宁夏回族自治区重点研发计划项目（2016KJHM29）资助.

Isolation, identification and degradation characteristics of a three phthalates degrading bacterium

  • 1. College of Resources and Environment, Ningxia University, Yinchuan, 750021, China;

  • 2. College of Economics and Management, Ningxia University, Yinchuan, 750021, China;

  • 3. Key Laboratory(China-Arab) of Resource Evaluation and Environmental Regulation of Arid Region in Ningxia, Yinchuan, 750021, China

    • Corresponding author: WANG Yajuan, 358437013@qq.com ; 
  • Fund Project: Supported by the Key Research Plan Program of the Ningxia Hui Autonomous Region(2016KJHM29).

  • 摘要:

    选择邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二丁酯（DnBP）和邻苯二甲酸二（2-乙基己基）酯（DEHP）作为目标污染物，采用富集驯化法从设施菜地土壤中筛选出1株可同时降解DMP、DnBP和DEHP的细菌MB1.经形态、生理生化特征及16S rDNA序列分析，初步鉴定为微杆菌属（Microbacterium sp.）.通过正交试验研究了该菌株的最优降解条件以及最优条件下该菌株的生长曲线和降解曲线，最后在培养条件下研究了该菌株对人工污染土壤中邻苯二甲酸酯的降解特性.结果表明，菌株MB1的最优降解条件为：pH值为8，温度为25℃，接菌量为5%，每种邻苯二甲酸酯浓度为300 mg·L-1.在此最优条件下该菌株呈S型曲线增长，7 d后无机盐培养液中DMP、DnBP和DEHP的降解率分别为99.62%%、99.65%和55.26%.人工污染土壤中空白试验和投加菌株试验结果为：在不添加菌液的处理中，灭菌土壤21 d时DMP、DnBP和DEHP的降解率分别为3.86%、4.19%和2.01%；未灭菌土壤21 d时对DMP、DnBP和DEHP的降解率分别为4.82%、5.99%和3.44%.在添加菌液的处理中，21 d时土壤灭菌处理中DMP、DnBP和DEHP的降解率分别达94.45%、95.65%和39.21%；而土壤未灭菌处理中DMP、DnBP和DEHP的降解率分别达94.93%、95.99%和41.16%.该结果表明：土壤中土著微生物仅能降解微量PAEs，菌株MB1对土壤中DMP、DnBP和DEHP等3种PAEs污染物具有较为高效的降解能力，未灭菌土壤中邻苯二甲酸酯的降解效果略高于灭菌土壤.

    Abstract:

    A strain, named MB1, capable of simultaneously degrading dimethyl phthalate(DMP),dibutyl phthalate (DnBP) and di-(2-ethylhexyl) phthalate (DEHP) and was isolated by culture enrichment method from facility vegetable soil. Based on the morphology, physiological characteristic and 16S rDNA sequence analysis, MB1 was identified as Microbacterium sp. and the optimal degradation conditions were studied by orthogonal experiment design. And growth curve, degradation curve of strain MB1 were investigated under optimal degradation conditions. Finally, the strain MB1 to phthalic acid esters degradation characteristics in artificially contaminated soil was investigated in culture incubation. The results showed that the optimized degradation condition was pH=8, temperature 25℃, inoculum size (V/V) 5% and 300 mg·L-1 of original PAEs concentration. Under the optimal condition, the strain presented shaped growth curve, and the degradation rate of DMP, DnBP and DEHP were 99.62%, 99.65% and 55.26% in mineral salt medium after 7 days, respectively. The degradation rates of DMP, DnBP and DEHP were 3.86%, 4.19% and 2.01% in the sterilized soil and 4.82%, 5.99% and 3.44% in the unsterilized soil without adding strain after 21 days, respectively. The degradation rate of DMP, DnBP and DEHP were 94.45%, 95.65% and 39.21% in sterilized soil and 94.93%, 95.99% and 41.16% in unsterilized soil by adding strain after 21 days, respectively. The results showed that:The strain MB1 had a relatively high degradation capacity for DMP, DnBP and DEHP in soil, and indigenous microbes degraded only a small amount of PAEs in soil. Degradation of phthalates by the strain MB1 was slightly higher in the unsterilized soil than in the sterilized soil.

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  • 收稿日期:  2019-01-04
  • 刊出日期:  2019-12-10

一株同时降解3种邻苯二甲酸酯降解菌的筛选鉴定及降解特性

    通讯作者: 王亚娟, E-mail: 358437013@qq.com ; 
  • 1. 宁夏大学资源环境学院, 银川, 750021;
  • 2. 宁夏大学经济管理学院, 银川, 750021;
  • 3. 宁夏(中阿)旱区资源评价与环境调控重点实验室, 银川, 750021
  • 收稿日期:  2019-01-04
  • 网络出版日期:  2019-12-10
基金项目:

宁夏回族自治区重点研发计划项目（2016KJHM29）资助.

摘要: 

选择邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二丁酯（DnBP）和邻苯二甲酸二（2-乙基己基）酯（DEHP）作为目标污染物，采用富集驯化法从设施菜地土壤中筛选出1株可同时降解DMP、DnBP和DEHP的细菌MB1.经形态、生理生化特征及16S rDNA序列分析，初步鉴定为微杆菌属（Microbacterium sp.）.通过正交试验研究了该菌株的最优降解条件以及最优条件下该菌株的生长曲线和降解曲线，最后在培养条件下研究了该菌株对人工污染土壤中邻苯二甲酸酯的降解特性.结果表明，菌株MB1的最优降解条件为：pH值为8，温度为25℃，接菌量为5%，每种邻苯二甲酸酯浓度为300 mg·L-1.在此最优条件下该菌株呈S型曲线增长，7 d后无机盐培养液中DMP、DnBP和DEHP的降解率分别为99.62%%、99.65%和55.26%.人工污染土壤中空白试验和投加菌株试验结果为：在不添加菌液的处理中，灭菌土壤21 d时DMP、DnBP和DEHP的降解率分别为3.86%、4.19%和2.01%；未灭菌土壤21 d时对DMP、DnBP和DEHP的降解率分别为4.82%、5.99%和3.44%.在添加菌液的处理中，21 d时土壤灭菌处理中DMP、DnBP和DEHP的降解率分别达94.45%、95.65%和39.21%；而土壤未灭菌处理中DMP、DnBP和DEHP的降解率分别达94.93%、95.99%和41.16%.该结果表明：土壤中土著微生物仅能降解微量PAEs，菌株MB1对土壤中DMP、DnBP和DEHP等3种PAEs污染物具有较为高效的降解能力，未灭菌土壤中邻苯二甲酸酯的降解效果略高于灭菌土壤.

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