双酚A对小鼠肝脏和肾脏细胞的氧化损伤

付宇; 王慧玉; 可尘娟; 王景明; 熊国梅; 杨旭

生态毒理学报

双酚A对小鼠肝脏和肾脏细胞的氧化损伤

, , , , ,

付宇, 王慧玉, 可尘娟, 王景明, 熊国梅, 杨旭. 双酚A对小鼠肝脏和肾脏细胞的氧化损伤[J]. 生态毒理学报, 2012, 7(4): 360-366.

引用本文:

付宇, 王慧玉, 可尘娟, 王景明, 熊国梅, 杨旭. 双酚A对小鼠肝脏和肾脏细胞的氧化损伤[J]. 生态毒理学报, 2012, 7(4): 360-366.

Fu Yu, Wang Huiyu, Ke Chenjuan, Wang Jingming, Xiong Guomei, Yang Xu. Oxidative Damage of Bisphenol A to Mouse Hepatic and Nephric Cells in Vivo[J]. Asian journal of ecotoxicology, 2012, 7(4): 360-366.

Citation:

Fu Yu, Wang Huiyu, Ke Chenjuan, Wang Jingming, Xiong Guomei, Yang Xu. Oxidative Damage of Bisphenol A to Mouse Hepatic and Nephric Cells in Vivo[J]. Asian journal of ecotoxicology, 2012, 7(4): 360-366.

双酚A对小鼠肝脏和肾脏细胞的氧化损伤

华中师范大学生命科学学院湖北省遗传调控与整合生物学重点实验室, 武汉 430079

作者简介: 付宇(1990-),男,学士,研究方向为生物化学,E-mail:fuyu505642275@yahoo.cn;王慧玉(1990-),女,学士,研究方向为分子生物学,E-mail:wanghuiyu894683557@yahoo.cn

基金项目:
国家自然科学基金(51136002

51076079)
中图分类号: X171.5;R994.6

Oxidative Damage of Bisphenol A to Mouse Hepatic and Nephric Cells in Vivo

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan 430079, China
Fund Project:

摘要: 为探究双酚A (BPA)的氧化毒性,分别以剂量为20、40和80 mg·kg^-1·d^-1的BPA对雄性昆明小鼠灌胃处理1周,并测定了小鼠体内活性氧自由基(ROS)水平、还原型谷胱甘肽(GSH)含量、丙二醛(MDA)含量和DNA-蛋白质交联系数(DPC)。与对照组相比,各BPA暴露组小鼠肝脏和肾脏细胞中的ROS生成量、MDA含量和DPC系数均升高,而GSH含量下降(P < 0.05或P < 0.01)。ROS生成量、GSH含量和DPC系数均显示出剂量-效应关系。研究表明,BPA可扰乱小鼠肝脏和肾脏细胞的氧化应激平衡,诱导细胞氧化损伤。
- 双酚A /
- 昆明小鼠 /
- 活性氧 /
- 还原型谷胱甘肽 /
- 丙二醛 /
- DNA蛋白质交联系数 /
- 氧化损伤
Abstract: To investigate the oxidative toxicity of bisphenol A (BPA), Kunming male mice were fed daily with BPA at the doses of 20, 40 and 80 mg·kg^-1·d^-1 for one week. The reactive oxygen species (ROS) level, glutathione (GSH) content, malondialdehyde (MDA) content and DNA-protein cross-linking (DPC) coefficient of hepatocytes and nephrocytes were detected to estimate the oxidative stress in the liver and kidney of male mice. Compared with the control, ROS generation, MDA content and DPC coefficient increased while GSH content decreased in BPA exposure groups (P < 0.05 or P < 0.01). ROS level, GSH content and DPC coefficient all showed doseeffect relationships. It is demonstrated that BPA could induce oxidative damage by disturbing the balance between ROS and antioxidant defense system in liver and kidney of mice.
- bisphenol A /
- Kuming mice /
- reactive oxygen species (ROS) /
- glutathione (GSH) /
- malondialdehyde (MDA) /
- DNA-protein cross-linking coefficient (DPC) /
- oxidative damage

点击查看大图

计量

文章访问数: 988
HTML全文浏览数: 988
PDF下载数: 20
施引文献: 0

付宇, 王慧玉, 可尘娟, 王景明, 熊国梅, 杨旭. 双酚A对小鼠肝脏和肾脏细胞的氧化损伤[J]. 生态毒理学报, 2012, 7(4): 360-366.

引用本文:

付宇, 王慧玉, 可尘娟, 王景明, 熊国梅, 杨旭. 双酚A对小鼠肝脏和肾脏细胞的氧化损伤[J]. 生态毒理学报, 2012, 7(4): 360-366.

Citation:

双酚A对小鼠肝脏和肾脏细胞的氧化损伤

作者简介: 付宇(1990-),男,学士,研究方向为生物化学,E-mail:fuyu505642275@yahoo.cn;王慧玉(1990-),女,学士,研究方向为分子生物学,E-mail:wanghuiyu894683557@yahoo.cn
华中师范大学生命科学学院湖北省遗传调控与整合生物学重点实验室, 武汉 430079

收稿日期: 2012-05-14

基金项目:

国家自然科学基金(51136002

51076079)

关键词:

摘要: 为探究双酚A (BPA)的氧化毒性,分别以剂量为20、40和80 mg·kg^-1·d^-1的BPA对雄性昆明小鼠灌胃处理1周,并测定了小鼠体内活性氧自由基(ROS)水平、还原型谷胱甘肽(GSH)含量、丙二醛(MDA)含量和DNA-蛋白质交联系数(DPC)。与对照组相比,各BPA暴露组小鼠肝脏和肾脏细胞中的ROS生成量、MDA含量和DPC系数均升高,而GSH含量下降(P < 0.05或P < 0.01)。ROS生成量、GSH含量和DPC系数均显示出剂量-效应关系。研究表明,BPA可扰乱小鼠肝脏和肾脏细胞的氧化应激平衡,诱导细胞氧化损伤。

English Abstract

Oxidative Damage of Bisphenol A to Mouse Hepatic and Nephric Cells in Vivo

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan 430079, China

Received Date: 2012-05-14

Fund Project:

Keywords:

bisphenol A /
Kuming mice /
reactive oxygen species (ROS) /
glutathione (GSH) /
malondialdehyde (MDA) /
DNA-protein cross-linking coefficient (DPC) /
oxidative damage

Abstract: To investigate the oxidative toxicity of bisphenol A (BPA), Kunming male mice were fed daily with BPA at the doses of 20, 40 and 80 mg·kg^-1·d^-1 for one week. The reactive oxygen species (ROS) level, glutathione (GSH) content, malondialdehyde (MDA) content and DNA-protein cross-linking (DPC) coefficient of hepatocytes and nephrocytes were detected to estimate the oxidative stress in the liver and kidney of male mice. Compared with the control, ROS generation, MDA content and DPC coefficient increased while GSH content decreased in BPA exposure groups (P < 0.05 or P < 0.01). ROS level, GSH content and DPC coefficient all showed doseeffect relationships. It is demonstrated that BPA could induce oxidative damage by disturbing the balance between ROS and antioxidant defense system in liver and kidney of mice.