高级搜索

厌氧氨氧化耦合异养反硝化的脱氮性能及污泥性状

,  ,  ,  ,  , 

downloadPDF

上一篇

下一篇

刘常敬, 李泽兵, 郑照明, 王昌稳, 赵白航, 李军. 厌氧氨氧化耦合异养反硝化的脱氮性能及污泥性状[J]. 环境工程学报, 2014, 8(8): 3137-3142.
引用本文: 刘常敬, 李泽兵, 郑照明, 王昌稳, 赵白航, 李军. 厌氧氨氧化耦合异养反硝化的脱氮性能及污泥性状[J]. 环境工程学报, 2014, 8(8): 3137-3142.
shu
Liu Changjing, Li Zebing, Zheng Zhaoming, Wang Changwen, Zhao Baihang, Li Jun. Nitrogen removal performance and sludge characteristics of anammox coupling heterotrophic denitrification[J]. Chinese Journal of Environmental Engineering, 2014, 8(8): 3137-3142.
Citation: Liu Changjing, Li Zebing, Zheng Zhaoming, Wang Changwen, Zhao Baihang, Li Jun. Nitrogen removal performance and sludge characteristics of anammox coupling heterotrophic denitrification[J]. Chinese Journal of Environmental Engineering, 2014, 8(8): 3137-3142.
shu

厌氧氨氧化耦合异养反硝化的脱氮性能及污泥性状

  • 1.

    北京工业大学水质科学与水环境恢复工程北京市重点实验室, 北京 100124

  • 2.

    东华理工大学水资源与环境工程学院, 南昌 330013

  • 基金项目:

    国家自然科学基金资助项目（51078008）

    北京市自然科学基金资助项目（8122005）

    北京工业大学研究生科技基金资助项目（ykj-2012-8496）

  • 中图分类号: X703

Nitrogen removal performance and sludge characteristics of anammox coupling heterotrophic denitrification

  • 1.

    Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China

  • 2.

    School of Water Resources and Environmental Engineering, East China Institute of Technology, Nanchang 330013, China

  • Fund Project:

  • 摘要: 通过连续实验和血清瓶批式实验研究了厌氧氨氧化耦合异养反硝化的代谢特性。在pH 7.8、温度25℃左右、水力停留时间1.5 h和苯酚浓度18.82 mg/L的条件下，耦合反应器能长期稳定运行。结果表明，NH4+-N、NO2--N去除率高达100%，TN去除率为87.51%。消耗的NH4+-N、NO2--N与生成的NO3--N之比为1：1.49：0.12，平均总氮容积负荷为2.53 kg/（m3·d），平均总氮去除负荷可达2.26 kg/（m3·d）。系统内异养反硝化与厌氧氨氧化存在协同和竞争关系，总氮的去除是异养反硝化菌和厌氧氨氧化菌共同作用的结果。耦合系统中ANAMMOX对TN去除贡献率达到86.72%，异养反硝化对TN去除贡献率达到13.28%（其中以NO2--N为电子受体的反硝化比例为7.16%，以NO3--N为电子受体的反硝化比例为5.89%）。污泥性状研究表明，颗粒污泥存在3种形式：一种是ANAMMOX颗粒污泥；一种是苯酚反硝化颗粒污泥；一种是ANAMMOX菌外面包裹苯酚反硝化菌的颗粒污泥。另外，颗粒污泥的无机组分较高。污泥扫描电镜照片显示厌氧氨氧化菌为球状，反硝化菌为短杆状。
    Abstract: The metabolism of anammox coupling heterotrophic denitrification process was investigated by continuous cultivation and serum bottle batch tests. Under the conditions of pH of 7.8, water temperature of 25℃, hydraulic detention time (HRT) of 1.5 h and phenol concentration of 18.82 mg/L, coupling reactor could operate stably for a long time. The results show that the removal rates of ammonia and nitrite are as high as 100%, respectively, while the removal rate of TN is 87.51%. The ratio of removed NH4+-N, reomved NO2--N and generated NO3--N is 1:1.49:0.12, the average total nitrogen volumetric loading rate is 2.53/kg(m3·d), and the average total nitrogen volumetric removal rate reaches 2.26 kg/(m3·d). The relationship between heterotrophic denitrification and anammox is cooperative and competitive in the system while total nitrogen is removed through the joint action of denitrifying bacteria and ANAMMOX bacteria. In the coupling system, the proportion of nitrogen removal through ANAMMOX is 86.72% of the total nitrogen removal, however, the heterotrophic denitrification takes up 13.28% (the proportion of denitrification using NO2--N as the electron acceptor is 7.16%, the proportion of denitrification using NO3--N as the electron acceptor is 5.89%). The study of sludge character indicates that the granular sludge has three types: ANAMMOX granular sludge, phenol denitrifying granular sludge and ANAMMOX granular sludge coated by phenol denitrifying bacteria. In addition, the granular sludge has a high inorganic component. The sludge scanning electron microscope photos illustrate that ANAMMOX bacteria is spherical and denitrification bacteria is short rod.
  • 加载中
  • [1] Maranon E., Vazquez I., Rodriguez J., et al. Coke wastewater treatment by a three-step activated sludge system. Water, Air and Soil Pollution,2008,192(1-4): 155-164
    [2] A.F. Ramosa, M.A. G'omez, E. Hontoria, et al. Biological nitrogen and phenol removal from saline industrial wastewater by submerged fixed-film reactor. Journal of Hazardous Materials,2007,142(1-2):175-183
    [3] Mulder A., van de Graaf A. A., Robertsonb L. A., et al. Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor. FEMS Microbiology Ecology,1995,16(3):177-183
    [4] Strous M., Heijnen J. J., Kuenen J. G., et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium oxidizing microorganisms. Appl. Microbiol. Biotechnol.,1998,50(5): 589-596
    [5] Jian-Hang Zhu,Jin-Ping Lin, Bao Zhang, et al. Simultaneous removal of phenol and nitrate in an anaerobic bioreactor. Environ. Eng., 2006,132(9):1073-1077
    [6] Toh S. K.,Ashbolt N. J. Adaptation of anaerobic ammonium-oxidising consortium to synthetic coke-ovens wastewater. Applied Microbiology and Biotechnology,2002,59(2/3):344-352
    [7] 林琳,李玉平,曹宏斌,等. 焦化废水厌氧氨氧化生物脱氮的研究. 中国环境科学,2010,30(9):1201-1206 Lin Lin, Li Yuping, Cao Hongbin, et al. Anaerobic ammonium oxidation (ANAMMOX) for biological ntrogen removal from coking wastewater. China Environmental Science, 2010,30(9):1201-1206 (in Chinese)
    [8] 李泽兵,刘常敬,赵白航,等. 多基质时厌氧氨氧化菌、异养反硝化污泥活性及抑制特征. 中国环境科学,2013,33(4):648-654 Li Zebing, Liu Changjing, Zhao Baihang, et al. Activity and inhibition characteristics of anammox and heterotrophic denitrifier bacteria in a multi-substrate. China Environmental Science, 2013,33(4):648-654 (in Chinese)
    [9] Strous M., Heijnen J. J., Kuenen J. G., et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium oxidizing microorganisms. Appl. Microbiol. Biotechnol.,1998, 50(5): 589-596
    [10] Ricardo Beristain-Cardoso, Anne-Claire Texier, Angel Alpuche-Solis,et al. Phenol and sulfide oxidation in a denitrifying biofilm reactor and its microbial community analysis. Process Biochemistry, 2009,44 (1):23-28
    [11] 张少辉,李聪. 有机碳源条件下的厌氧氨氧化研究. 武汉理工大学学报,2011,33(3):130-135 Zhang Shaohui, Li Cong. Anammox Process in the presence of organic carbon source. Journal of Wuhan University of Technology, 2011,33(3):130-135(in Chinese)
    [12] 胡勇有,梁辉强,朱静平,等. 有机碳源环境下的厌氧氨氧化批式实验. 华南理工大学学报(自然科学版), 2007,35(6):116-119 Hu Yongyou, Liang Huiqiang, Zhu Jingping, et al. The batch experiment of anammox in the presence of organic carbon source. Journal of South China University of Technology(Natural Science), 2007,35(6):116-119(in Chinese)
    [13] Strous M., Heijnen J. J., Kuenen J. G., et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms. Mocrobiol. Biotechnol., 1996, 50(5): 589-596
    [14] Van de Graaf A. A., De Bruijn P., Robertson L. A., et al.Autotrophlic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor. Microbiology,1996, 142(8):2187-2196
    [15] 吴芳芳,邱凌峰. 苯酚降解菌TX1的分离鉴定及其代谢途径. 福州大学学报(自然科学版),2010,38(5):767-772 Wu Fangfang, Qiu Lingfeng Isolation. Identification and metabolic pathway of phenol-degrading Trichosporon sp.X1. Journal of Fuzhou University (Natural Science), 2010,38(5):767-772(in Chinese)
    [16] Gallert C., Knoll G., Winter J. Anaerobic carboxylation of phenol to benzoate: Use of deuterated phenols revealed carboxylation exclusively in the C4-position. Appl. Microbiol. Biotechnol.,1991, 36(1):124-129
    [17] Ke S., Shi Z., Zhang T., et al. Degradation of phenol in an upflow anaerobic sludge blanket (UASB) reactor at ambient temperature. J. Environ. Sci., 2004, 16 (3):525-528
    [18] H.H.P. Fang, D.W. Liang, T. Zhang, et al. Anaerobic treatment of phenol in wastewater under thermophilic condition. Water Research,2006,40 (3):427-434
    [19] 吴艳阳,吴群河,黄珊,等. 沉积物中多环芳烃对反硝化功能基因垂直分布的影响. 环境科学,2012,33(10):3592-3597 Wu Yanyang, Wu Qunhe, Huang Shan, et al. Effect of polycyclic aromatic hydrocarbons on the vertical distribution of denitrifying genes in river sediments. Environmental Science, 2012,33(10):3592-3597(in Chinese)
    [20] van Niftrik L.,Geerts W. J. C.,van Donselaar E. G., et al. Linking ultrastrueture and function in four genera of anaerobic ammonium-oxidizing baeteria:Cell plan,glycogen storage,and localization of cytochrome C proteins. Journal of Bacteriology,2008,190(2):708-717
    [21] Guven D., Dapena A., Kartal B., et al. Propionate oxidation by and methanol inhibition of anaerobic ammonium-oxidizing bacteria. Applied and Environmental Microbiology,2005,71(2): 1066-1071
    [22] Rattray J. E., Geenevasen J. A. J., Van Niftrik L. A., et al. Carbon isotope-labelling experiments indicate that ladderane lipids of anammox bacteria are synthesized by a previously undescribed novel pathway. FEMS Microbiology Letters,2009,292(1): 115-122
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  1584
  • HTML全文浏览数:  939
  • PDF下载数:  829
  • 施引文献:  0
出版历程
  • 收稿日期:  2013-08-06
  • 刊出日期:  2014-07-31
刘常敬, 李泽兵, 郑照明, 王昌稳, 赵白航, 李军. 厌氧氨氧化耦合异养反硝化的脱氮性能及污泥性状[J]. 环境工程学报, 2014, 8(8): 3137-3142.
引用本文: 刘常敬, 李泽兵, 郑照明, 王昌稳, 赵白航, 李军. 厌氧氨氧化耦合异养反硝化的脱氮性能及污泥性状[J]. 环境工程学报, 2014, 8(8): 3137-3142.
shu
Liu Changjing, Li Zebing, Zheng Zhaoming, Wang Changwen, Zhao Baihang, Li Jun. Nitrogen removal performance and sludge characteristics of anammox coupling heterotrophic denitrification[J]. Chinese Journal of Environmental Engineering, 2014, 8(8): 3137-3142.
Citation: Liu Changjing, Li Zebing, Zheng Zhaoming, Wang Changwen, Zhao Baihang, Li Jun. Nitrogen removal performance and sludge characteristics of anammox coupling heterotrophic denitrification[J]. Chinese Journal of Environmental Engineering, 2014, 8(8): 3137-3142.
shu

厌氧氨氧化耦合异养反硝化的脱氮性能及污泥性状

  • 1. 北京工业大学水质科学与水环境恢复工程北京市重点实验室, 北京 100124
  • 2. 东华理工大学水资源与环境工程学院, 南昌 330013
  • 收稿日期:  2013-08-06
基金项目:

国家自然科学基金资助项目（51078008）

北京市自然科学基金资助项目（8122005）

北京工业大学研究生科技基金资助项目（ykj-2012-8496）

摘要: 通过连续实验和血清瓶批式实验研究了厌氧氨氧化耦合异养反硝化的代谢特性。在pH 7.8、温度25℃左右、水力停留时间1.5 h和苯酚浓度18.82 mg/L的条件下，耦合反应器能长期稳定运行。结果表明，NH4+-N、NO2--N去除率高达100%，TN去除率为87.51%。消耗的NH4+-N、NO2--N与生成的NO3--N之比为1：1.49：0.12，平均总氮容积负荷为2.53 kg/（m3·d），平均总氮去除负荷可达2.26 kg/（m3·d）。系统内异养反硝化与厌氧氨氧化存在协同和竞争关系，总氮的去除是异养反硝化菌和厌氧氨氧化菌共同作用的结果。耦合系统中ANAMMOX对TN去除贡献率达到86.72%，异养反硝化对TN去除贡献率达到13.28%（其中以NO2--N为电子受体的反硝化比例为7.16%，以NO3--N为电子受体的反硝化比例为5.89%）。污泥性状研究表明，颗粒污泥存在3种形式：一种是ANAMMOX颗粒污泥；一种是苯酚反硝化颗粒污泥；一种是ANAMMOX菌外面包裹苯酚反硝化菌的颗粒污泥。另外，颗粒污泥的无机组分较高。污泥扫描电镜照片显示厌氧氨氧化菌为球状，反硝化菌为短杆状。

English Abstract

    全文HTML

参考文献 (22)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心