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厌氧氨氧化耦合异养反硝化的脱氮性能及污泥性状

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刘常敬, 李泽兵, 郑照明, 王昌稳, 赵白航, 李军. 厌氧氨氧化耦合异养反硝化的脱氮性能及污泥性状[J]. 环境工程学报, 2014, 8(8): 3137-3142.
引用本文: 刘常敬, 李泽兵, 郑照明, 王昌稳, 赵白航, 李军. 厌氧氨氧化耦合异养反硝化的脱氮性能及污泥性状[J]. 环境工程学报, 2014, 8(8): 3137-3142.
shu
Liu Changjing, Li Zebing, Zheng Zhaoming, Wang Changwen, Zhao Baihang, Li Jun. Nitrogen removal performance and sludge characteristics of anammox coupling heterotrophic denitrification[J]. Chinese Journal of Environmental Engineering, 2014, 8(8): 3137-3142.
Citation: Liu Changjing, Li Zebing, Zheng Zhaoming, Wang Changwen, Zhao Baihang, Li Jun. Nitrogen removal performance and sludge characteristics of anammox coupling heterotrophic denitrification[J]. Chinese Journal of Environmental Engineering, 2014, 8(8): 3137-3142.
shu

厌氧氨氧化耦合异养反硝化的脱氮性能及污泥性状

  • 1.

    北京工业大学水质科学与水环境恢复工程北京市重点实验室, 北京 100124

  • 2.

    东华理工大学水资源与环境工程学院, 南昌 330013

  • 基金项目:

    国家自然科学基金资助项目（51078008）

    北京市自然科学基金资助项目（8122005）

    北京工业大学研究生科技基金资助项目（ykj-2012-8496）

  • 中图分类号: X703

Nitrogen removal performance and sludge characteristics of anammox coupling heterotrophic denitrification

  • 1.

    Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China

  • 2.

    School of Water Resources and Environmental Engineering, East China Institute of Technology, Nanchang 330013, China

  • Fund Project:

  • 摘要: 通过连续实验和血清瓶批式实验研究了厌氧氨氧化耦合异养反硝化的代谢特性。在pH 7.8、温度25℃左右、水力停留时间1.5 h和苯酚浓度18.82 mg/L的条件下，耦合反应器能长期稳定运行。结果表明，NH4+-N、NO2--N去除率高达100%，TN去除率为87.51%。消耗的NH4+-N、NO2--N与生成的NO3--N之比为1：1.49：0.12，平均总氮容积负荷为2.53 kg/（m3·d），平均总氮去除负荷可达2.26 kg/（m3·d）。系统内异养反硝化与厌氧氨氧化存在协同和竞争关系，总氮的去除是异养反硝化菌和厌氧氨氧化菌共同作用的结果。耦合系统中ANAMMOX对TN去除贡献率达到86.72%，异养反硝化对TN去除贡献率达到13.28%（其中以NO2--N为电子受体的反硝化比例为7.16%，以NO3--N为电子受体的反硝化比例为5.89%）。污泥性状研究表明，颗粒污泥存在3种形式：一种是ANAMMOX颗粒污泥；一种是苯酚反硝化颗粒污泥；一种是ANAMMOX菌外面包裹苯酚反硝化菌的颗粒污泥。另外，颗粒污泥的无机组分较高。污泥扫描电镜照片显示厌氧氨氧化菌为球状，反硝化菌为短杆状。
    Abstract: The metabolism of anammox coupling heterotrophic denitrification process was investigated by continuous cultivation and serum bottle batch tests. Under the conditions of pH of 7.8, water temperature of 25℃, hydraulic detention time (HRT) of 1.5 h and phenol concentration of 18.82 mg/L, coupling reactor could operate stably for a long time. The results show that the removal rates of ammonia and nitrite are as high as 100%, respectively, while the removal rate of TN is 87.51%. The ratio of removed NH4+-N, reomved NO2--N and generated NO3--N is 1:1.49:0.12, the average total nitrogen volumetric loading rate is 2.53/kg(m3·d), and the average total nitrogen volumetric removal rate reaches 2.26 kg/(m3·d). The relationship between heterotrophic denitrification and anammox is cooperative and competitive in the system while total nitrogen is removed through the joint action of denitrifying bacteria and ANAMMOX bacteria. In the coupling system, the proportion of nitrogen removal through ANAMMOX is 86.72% of the total nitrogen removal, however, the heterotrophic denitrification takes up 13.28% (the proportion of denitrification using NO2--N as the electron acceptor is 7.16%, the proportion of denitrification using NO3--N as the electron acceptor is 5.89%). The study of sludge character indicates that the granular sludge has three types: ANAMMOX granular sludge, phenol denitrifying granular sludge and ANAMMOX granular sludge coated by phenol denitrifying bacteria. In addition, the granular sludge has a high inorganic component. The sludge scanning electron microscope photos illustrate that ANAMMOX bacteria is spherical and denitrification bacteria is short rod.
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  • 收稿日期:  2013-08-06
  • 刊出日期:  2014-07-31
刘常敬, 李泽兵, 郑照明, 王昌稳, 赵白航, 李军. 厌氧氨氧化耦合异养反硝化的脱氮性能及污泥性状[J]. 环境工程学报, 2014, 8(8): 3137-3142.
引用本文: 刘常敬, 李泽兵, 郑照明, 王昌稳, 赵白航, 李军. 厌氧氨氧化耦合异养反硝化的脱氮性能及污泥性状[J]. 环境工程学报, 2014, 8(8): 3137-3142.
shu
Liu Changjing, Li Zebing, Zheng Zhaoming, Wang Changwen, Zhao Baihang, Li Jun. Nitrogen removal performance and sludge characteristics of anammox coupling heterotrophic denitrification[J]. Chinese Journal of Environmental Engineering, 2014, 8(8): 3137-3142.
Citation: Liu Changjing, Li Zebing, Zheng Zhaoming, Wang Changwen, Zhao Baihang, Li Jun. Nitrogen removal performance and sludge characteristics of anammox coupling heterotrophic denitrification[J]. Chinese Journal of Environmental Engineering, 2014, 8(8): 3137-3142.
shu

厌氧氨氧化耦合异养反硝化的脱氮性能及污泥性状

  • 1. 北京工业大学水质科学与水环境恢复工程北京市重点实验室, 北京 100124
  • 2. 东华理工大学水资源与环境工程学院, 南昌 330013
  • 收稿日期:  2013-08-06
基金项目:

国家自然科学基金资助项目（51078008）

北京市自然科学基金资助项目（8122005）

北京工业大学研究生科技基金资助项目（ykj-2012-8496）

摘要: 通过连续实验和血清瓶批式实验研究了厌氧氨氧化耦合异养反硝化的代谢特性。在pH 7.8、温度25℃左右、水力停留时间1.5 h和苯酚浓度18.82 mg/L的条件下，耦合反应器能长期稳定运行。结果表明，NH4+-N、NO2--N去除率高达100%，TN去除率为87.51%。消耗的NH4+-N、NO2--N与生成的NO3--N之比为1：1.49：0.12，平均总氮容积负荷为2.53 kg/（m3·d），平均总氮去除负荷可达2.26 kg/（m3·d）。系统内异养反硝化与厌氧氨氧化存在协同和竞争关系，总氮的去除是异养反硝化菌和厌氧氨氧化菌共同作用的结果。耦合系统中ANAMMOX对TN去除贡献率达到86.72%，异养反硝化对TN去除贡献率达到13.28%（其中以NO2--N为电子受体的反硝化比例为7.16%，以NO3--N为电子受体的反硝化比例为5.89%）。污泥性状研究表明，颗粒污泥存在3种形式：一种是ANAMMOX颗粒污泥；一种是苯酚反硝化颗粒污泥；一种是ANAMMOX菌外面包裹苯酚反硝化菌的颗粒污泥。另外，颗粒污泥的无机组分较高。污泥扫描电镜照片显示厌氧氨氧化菌为球状，反硝化菌为短杆状。

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