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微生物絮凝剂的制备及其对城市污水厂污泥的脱水

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张峰, 尹华, 叶锦韶, 彭辉, 杨思敏, 刘京. 微生物絮凝剂的制备及其对城市污水厂污泥的脱水[J]. 环境工程学报, 2014, 8(5): 1927-1932.
引用本文: 张峰, 尹华, 叶锦韶, 彭辉, 杨思敏, 刘京. 微生物絮凝剂的制备及其对城市污水厂污泥的脱水[J]. 环境工程学报, 2014, 8(5): 1927-1932.
shu
Zhang Feng, Yin Hua, Ye Jinshao, Peng Hui, Yang Simin, Liu Jing. Microbial flocculant preperation and its application in dewatering of sewage treatment plant sludge[J]. Chinese Journal of Environmental Engineering, 2014, 8(5): 1927-1932.
Citation: Zhang Feng, Yin Hua, Ye Jinshao, Peng Hui, Yang Simin, Liu Jing. Microbial flocculant preperation and its application in dewatering of sewage treatment plant sludge[J]. Chinese Journal of Environmental Engineering, 2014, 8(5): 1927-1932.
shu

微生物絮凝剂的制备及其对城市污水厂污泥的脱水

  • 1.

    暨南大学环境工程系, 广东省高校水土环境毒害性污染物防治与生物修复重点实验室, 广州 510632

  • 2.

    暨南大学资源环境与可持续发展研究所, 广州 510632

  • 3.

    华南理工大学环境与能源学院, 工业聚集区污染 控制与生态修复教育部重点实验室, 广州 510006

  • 4.

    暨南大学化学系, 广州 510632

  • 基金项目:

    广州市科技计划项目（2011J4300075）

    广州市环保局科技计划项目（穗财建（2012）399号）

    番禺区科技局科技计划项目（2010-专-12-06）

  • 中图分类号: X703

Microbial flocculant preperation and its application in dewatering of sewage treatment plant sludge

  • 1.

    Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Department of Environmental Engineering, Jinan University, Guangzhou 510632, China

  • 2.

    Institute of Resources, Environment & Sustainable Development, Jinan University, Guangzhou 510632, China

  • 3.

    Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, College of Environment and Energy, South China University of Technology, Guangzhou 510006, China

  • 4.

    Department of Chemistry, Jinan University, Guangzhou 510632, China

  • Fund Project:

  • 摘要: 研究利用甘蔗渣作为廉价原材料制备微生物絮凝剂并探讨其对城市污泥的脱水性能。按0.5%最佳接种量接种，并利用发酵罐进行批式发酵培养，培养60 h后的发酵液具有最佳絮凝效果，投加量为5.0 mg/L时较优，污泥脱水率从75.60%提高到84.2%，污泥含水率从95.82%降至76.21%。此时絮凝剂粗产量为1.16 g/L。培养108 h后，发酵液仍具有显著的絮凝效果，能使污泥含水率维持在76.81%左右。补料发酵实验表明，恒pH培养会抑制微生物分泌絮凝剂，最佳絮凝效果为72 h的发酵液，投加量5.0 mg/L，污泥含水率降至76.47%。通过补料以及不控制pH后，发酵液絮凝效果迅速上升，投加不同量的发酵液使污泥的含水率保持在76.22%~75.60%之间。综合来说，补料在能减少原料浪费的同时也可以有效地提高絮凝剂的絮凝效果。
    Abstract: Bioflocculant was produced by using sugarcane bagasse as a cheap raw material to culture Aspergillus Aspergillus and its ability to remove water from sewage sludge was studied. The results showed that with the optimum inoculation volume of 0.5%, the lowest moisture content of sludge achieved after 60 h fermentation through batch culture. At the best bioflocculant dosage of 5.0 mg/L, the dewatering rate of sludge increased from 75.6% to 84.2%, and its moisture content decreased from 95.82% to 76.21%, with crude bioflocculant yield of 1.16 g/L. The sludge still kept lower moisture content, remaining about 76.81% after 108 h batch fementation. The constant pH in the process of fementation would inhibit A. sojae to secrete bioflocculant during fed-batch fementation. Under this condition, the sludge moisture rate was 76.47% after culturing A. sojae for 72 h at the best dosage of 5.0 mg/L. The flocculating effect of fermention liquid rose rapidly after feeding new culture medium and stopping pH control. The sludge moisture rates under different bioflocculant dosages were kept between 76.22%~75.60%. Generally speaking, feeding new culture could both decrease the waste of raw material and improve the flocculating rate of flocculant efficiently.
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  • [1] Buthelezi S. P., Olaniran A. O., Pillay B. Production and characterization of bioflocculants from bacteria isolated from wastewater treatment plant in South Africa. Biotechnology and Bioprocess Engineering, 2010, 15(5): 874-881
    [2] 冯绮澜, 尹华, 秦华明, 等. 利用甘蔗渣制备微生物絮凝剂的预处理方法研究. 环境工程学报, 2011, 5(6): 1268-1272 Feng Q. L., Yin H., Qin H. M., et al. Research on the pretreatment method of sugarcane bagasse for production of bioflocculant. Chinese Journal of Environmental Engineering, 2011, 5(6): 1268-1272 (in Chinese)
    [3] Xia S. Q., Zhang Z. Q., Wang X. J., et al. Production and characterization of a bioflocculant by Proteus mirabilis TJ-1. Bioresource Technology, 2008, 99(14): 6520-6527
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    [7] 马放, 段姝悦, 孔祥震, 等. 微生物絮凝剂的研究现状及其发展趋势. 中国给水排水, 2012, 28(2): 14-17 Ma F., Duan S. Y., Kong X. Z., et al. Present status and development trend of studies on microbial flocculants. China Water & Wastewater, 2012, 28(2): 14-17 (in Chinese)
    [8] Hans S., Daan C., Olivier T., et al. Optimization of sewage sludge conditioning and pressure dewatering by statistical modelling. Water Research, 2008, 42(4-5): 1061-1074
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    [10] 赵凯, 许鹏举, 谷广烨. 3, 5-二硝基水杨酸比色法测定还原糖含量的研究. 食品科学, 2008, 29(8): 534-536 Zhao K., Xue P. J., Gu G. Y. Study on determination of reducing sugar content using 3, 5-dinitrosalicylic acid method. Food Science, 2008, 29(8): 534-536 (in Chinese)
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出版历程
  • 收稿日期:  2013-07-01
  • 刊出日期:  2014-05-06
张峰, 尹华, 叶锦韶, 彭辉, 杨思敏, 刘京. 微生物絮凝剂的制备及其对城市污水厂污泥的脱水[J]. 环境工程学报, 2014, 8(5): 1927-1932.
引用本文: 张峰, 尹华, 叶锦韶, 彭辉, 杨思敏, 刘京. 微生物絮凝剂的制备及其对城市污水厂污泥的脱水[J]. 环境工程学报, 2014, 8(5): 1927-1932.
shu
Zhang Feng, Yin Hua, Ye Jinshao, Peng Hui, Yang Simin, Liu Jing. Microbial flocculant preperation and its application in dewatering of sewage treatment plant sludge[J]. Chinese Journal of Environmental Engineering, 2014, 8(5): 1927-1932.
Citation: Zhang Feng, Yin Hua, Ye Jinshao, Peng Hui, Yang Simin, Liu Jing. Microbial flocculant preperation and its application in dewatering of sewage treatment plant sludge[J]. Chinese Journal of Environmental Engineering, 2014, 8(5): 1927-1932.
shu

微生物絮凝剂的制备及其对城市污水厂污泥的脱水

  • 1.  暨南大学环境工程系, 广东省高校水土环境毒害性污染物防治与生物修复重点实验室, 广州 510632
  • 2.  暨南大学资源环境与可持续发展研究所, 广州 510632
  • 3.  华南理工大学环境与能源学院, 工业聚集区污染 控制与生态修复教育部重点实验室, 广州 510006
  • 4.  暨南大学化学系, 广州 510632
  • 收稿日期:  2013-07-01
基金项目:

广州市科技计划项目（2011J4300075）

广州市环保局科技计划项目（穗财建（2012）399号）

番禺区科技局科技计划项目（2010-专-12-06）

摘要: 研究利用甘蔗渣作为廉价原材料制备微生物絮凝剂并探讨其对城市污泥的脱水性能。按0.5%最佳接种量接种，并利用发酵罐进行批式发酵培养，培养60 h后的发酵液具有最佳絮凝效果，投加量为5.0 mg/L时较优，污泥脱水率从75.60%提高到84.2%，污泥含水率从95.82%降至76.21%。此时絮凝剂粗产量为1.16 g/L。培养108 h后，发酵液仍具有显著的絮凝效果，能使污泥含水率维持在76.81%左右。补料发酵实验表明，恒pH培养会抑制微生物分泌絮凝剂，最佳絮凝效果为72 h的发酵液，投加量5.0 mg/L，污泥含水率降至76.47%。通过补料以及不控制pH后，发酵液絮凝效果迅速上升，投加不同量的发酵液使污泥的含水率保持在76.22%~75.60%之间。综合来说，补料在能减少原料浪费的同时也可以有效地提高絮凝剂的絮凝效果。

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