Keywords: 
• acid mine drainage /
• sulfate /
• sulfate-reducing bacteria method /
• new organic carbon sources /
• fermentation liquid /
• reaction conditions

Abstract: For the problem of that the sulfate-reducing bacteria (SRB) in the treatment of acid mine drainage was lack of effective organic carbon sources,the fermentation liquor of mixture was used as new organic carbon source to domesticate SRB in this article. It was mixed by municipal sewage, chicken feces and wood chips on the scale of 80:7:3. Research on the effectively of removing sulfate by SRB used new organic carbon sources as nutrients, at different CODcr/SO42-(C/S) ratios, pH values, initial sulfate concentrations and heavy metal ions(Fe2+, Mn2+, Cu2+, Zn2+) concentrations. The optimum reaction conditions of removing sulfate were determined. Results showed that the optimum reaction conditions were SRB inoculation amount of 8% under anaerobic condition, growth temperature of 35℃, rotational speed of 50 r/min, C/S ratio of 1.5~2.0, pH value of 6~7, initial sulfate concentration of lower than 3 000 mg/L, range of concentration of Fe2+ 100~300 mg/L and Mn2+ 35 mg/L. Under the conditions, the efficiency of removing sulfate could reach up to 90%. The efficiency of removing sulfate was enhanced at the Fe2+ concentration of ≤500 mg/L and Mn2+ of ≤140 mg/L, while the concentrations of Fe2+ (≥600 mg/L) and Mn2+(>140 mg/L) seriously inhibited the growth of SRB. Both of Cu2+ and Zn2+ effected SRB, the growth of SRB was inhibited at the concentration of Cu2+(>15 mg/L) and Zn2+ (>45 mg/L). The new organic source can be an excellent organic carbon source of SRB, also can achieve the goal of using waste to treat waste. The achievement provides a reference for practical applications.