视黄酸和多溴联苯醚联合暴露对斑马鱼运动行为的影响
Effects of Co-exposure to Retinoic Acid and PBDEs on Locomotor Behavior of Zebrafish (Danio rerio)
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摘要: 水环境中的多溴联苯醚(PBDEs)污染会对水生生物神经系统产生影响，而视黄酸(retinoic acid, RA)对机体的神经系统和肢体发育具有重要作用。本文研究了四溴联苯醚(BDE-47)或十溴联苯醚(BDE-209)单独暴露以及RA联合BDE-47或BDE-209暴露对斑马鱼运动行为的影响。研究表明在稳定光照、黑暗和光暗交替刺激3种不同环境条件下，5 μmol·L-1 BDE-47和3 μmol·L-1 BDE-209单独暴露均导致斑马鱼运动速度显著降低。当2 nmol·L-1 RA联合相同剂量的BDE-47或BDE-209暴露时，可使仔鱼在稳定光照和黑暗下的自由运动速度相对单独暴露时显著升高，在光暗刺激下的运动速度也比单独暴露时于一定程度上有所缓解。因此，视黄酸的存在可以对因BDE-47和BDE-209暴露引起的斑马鱼运动行为异常起到恢复作用，这种恢复作用的机制可能是通过中枢神经系统或感官系统发挥作用。Abstract: Polybrominated diphenyl ethers (PBDEs) have become widespread environmental pollutants that exert neurotoxicity in aquatic organisms. Retinoic acid (RA) plays essential roles in limb morphogenesis and development of the central nervous system. The objectives, therefore, of the present study were to investigate the impacts of exposure to PBDEs (BDE-47 or BDE-209) and/or RA on locomotor behavior in zebrafish. We found that 5 μmol·L-1 BDE-47 or 3 μmol·L-1 BDE-209 exposure independently cause hypoactivity in all light, all dark or dark-light cycling stimulation conditions, while 2 nmol·L-1 RA and PBDEs (5 μmol·L-1 BDE-47 or 3 μmol·L-1 BDE-209) combined exposure lead to increased swimming speed compared with single exposure. The results indicated that the abnormal locomotor behavior in zebrafish caused by PBDEs can be restored when RA exists, which probably works through the central nervous system or sensory system.
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Key words:
- retinoic acid /
- PBDEs /
- zebrafish /
- behavior
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