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应用生物配体模型研究阳离子及pH值对水稻锌毒性的影响

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李晓敏, 胡莹, 黄益宗, 刘云霞, 李季. 应用生物配体模型研究阳离子及pH值对水稻锌毒性的影响[J]. 环境化学, 2017, 36(8): 1724-1734. doi: 10.7524/j.issn.0254-6108.2017032005
引用本文: 李晓敏, 胡莹, 黄益宗, 刘云霞, 李季. 应用生物配体模型研究阳离子及pH值对水稻锌毒性的影响[J]. 环境化学, 2017, 36(8): 1724-1734. doi: 10.7524/j.issn.0254-6108.2017032005
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LI Xiaomin, HU Ying, HUANG Yizong, LIU Yunxia, LI Ji. Effects of cations and pH in nutrient solution on zinc toxicity to rice (Oryza sativa) root elongation by the biotic ligand model[J]. Environmental Chemistry, 2017, 36(8): 1724-1734. doi: 10.7524/j.issn.0254-6108.2017032005
Citation: LI Xiaomin, HU Ying, HUANG Yizong, LIU Yunxia, LI Ji. Effects of cations and pH in nutrient solution on zinc toxicity to rice (Oryza sativa) root elongation by the biotic ligand model[J]. Environmental Chemistry, 2017, 36(8): 1724-1734. doi: 10.7524/j.issn.0254-6108.2017032005
shu

应用生物配体模型研究阳离子及pH值对水稻锌毒性的影响

  • 1.

    中国科学院生态环境研究中心, 北京, 100085;

  • 2.

    农业部环境保护科研监测所, 天津, 300191

  • 基金项目:

    国家自然科学基金（21377152）资助.

Effects of cations and pH in nutrient solution on zinc toxicity to rice (Oryza sativa) root elongation by the biotic ligand model

  • 1.

    Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China;

  • 2.

    Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin, 300191, China

  • Fund Project: Supported by the National Nature Science Fund Project of China (21377152).

  • 摘要: 采用室内水培实验， 通过改变溶液中pH值及主要阳离子（Ca2+、Mg2+、Na+和K+）浓度研究锌对水稻的毒性，建立了Zn对水稻根伸长毒性的生物配体模型（Biotic ligand model，BLM）.研究结果表明，增加Ca2+、Mg2+和H+的活度均可以减缓Zn2+对水稻根伸长的毒性，而增加Na+、K+的活度对Zn2+的毒性影响不大；在低pH（4.5-6.0）条件下，主要是Zn2+对水稻根伸长产生毒性，在高pH（6.5-8.0）条件下，Zn2+和ZnOH+是主要的致毒形态.根据生物配体模型理论估算的Zn2+、ZnOH+、Ca2+、Mg2+和H+的生物配体络合的平衡常数分别为：lgKZnBL=4.97、lgKZnOHBL=5.30、lgKCaBL=2.96、lgKMgBL=3.30和lgKHBL=5.21.根据各平衡常数计算可得，当Zn结合水稻的生物配体位点达到73%之后，水稻根伸长的抑制率达50%（即f50=73%）.利用上述参数建立的生物配体模型预测的EC50值均在实测值的2倍变化范围之内，表明生物配体模型可以有效地预测锌对水稻根伸长的急性毒性.
    Abstract: Acute toxicity of zinc (Zn) to rice (Oryza sativa) root elongation was investigated by changing the pH value and major cation (Ca2+, Mg2+, Na+ and K+) concentrations to develop an appropriate biotic ligand model (BLM) in solution culture. The results showed that the toxicity of Zn2+ was reduced with increasing avtivities of Ca2+, Mg2+ and H+, while the avtivities of Na+ and K+ did not significantly affect the Zn2+ toxicity. The toxicity could be explained mainly by Zn2+ binding to a biotic ligand (BL) at pH from 4.5 to 6.0 and by the joint toxicity of Zn2+ and ZnOH+ at pH from 6.5 to 8.0. According to the biotic ligand model (BLM) concept, the conditional stability constants for the binding of Zn2+, ZnOH+, Ca2+, Mg2+ and H+ to the BL were lgKZnBL=4.97, lgKZnOHBL=5.30, lgKCaBL=2.96, lgKMgBL=3.30 and lgKHBL=5.21, respectively. It was calculated that on average 73% of BL sites were occupied by Zn2+ when the rice root elongation was inhibited by 50% (f50=73%). On the basis of these estimated parameters, the BLM developed in this study could predict EC50 within a factor of 2 of the observed EC50. It was implicated that Zn-BLM could predict reliably the toxicity of Zn to rice.
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  • 收稿日期:  2017-03-20
  • 刊出日期:  2017-08-15

应用生物配体模型研究阳离子及pH值对水稻锌毒性的影响

  • 1.  中国科学院生态环境研究中心, 北京, 100085;
  • 2.  农业部环境保护科研监测所, 天津, 300191
  • 收稿日期:  2017-03-20
基金项目:

国家自然科学基金（21377152）资助.

摘要: 采用室内水培实验， 通过改变溶液中pH值及主要阳离子（Ca2+、Mg2+、Na+和K+）浓度研究锌对水稻的毒性，建立了Zn对水稻根伸长毒性的生物配体模型（Biotic ligand model，BLM）.研究结果表明，增加Ca2+、Mg2+和H+的活度均可以减缓Zn2+对水稻根伸长的毒性，而增加Na+、K+的活度对Zn2+的毒性影响不大；在低pH（4.5-6.0）条件下，主要是Zn2+对水稻根伸长产生毒性，在高pH（6.5-8.0）条件下，Zn2+和ZnOH+是主要的致毒形态.根据生物配体模型理论估算的Zn2+、ZnOH+、Ca2+、Mg2+和H+的生物配体络合的平衡常数分别为：lgKZnBL=4.97、lgKZnOHBL=5.30、lgKCaBL=2.96、lgKMgBL=3.30和lgKHBL=5.21.根据各平衡常数计算可得，当Zn结合水稻的生物配体位点达到73%之后，水稻根伸长的抑制率达50%（即f50=73%）.利用上述参数建立的生物配体模型预测的EC50值均在实测值的2倍变化范围之内，表明生物配体模型可以有效地预测锌对水稻根伸长的急性毒性.

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