纳米ZnO对中华圆田螺的氧化应激效应
Oxidative stress of nano ZnO on Cipangopaludina cahayensis
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摘要: 以淡水螺为受试生物，研究了中华圆田螺（Cipangopaludina Cahayensis）肝脏中自由基强度、抗氧化酶活性和丙二醛（MDA）含量纳米ZnO暴露21 d时的变化情况.结果表明，纳米ZnO可显著诱导中华圆田螺产生羟基（·OH）自由基；超氧化物歧化酶（SOD）活性和MDA含量的变化趋势相同，0.1-1 mg·L-1暴露下活性随浓度增加逐渐增加，2 mg·L-1时有所回落；过氧化氢酶（CAT）活性呈诱导状态，随浓度增加活性缓慢增大；谷胱甘肽S-转移酶（GST）活性保持抑制状态；·OH信号强度与SOD活性和MDA含量的变化具有一致性；SOD活性和MDA含量激活率较高，CAT激活率则保持稳定.Abstract: The effects of nano ZnO on the intensity of free radical, antioxidant enzyme activity and malondialdehyde (MDA) content in the liver of Cipangopaludina Cahayensis following 21 d exposure were investigatedto. The Hy-droxyl radical (·OH) concentration was determined by electron paramagnetic resonance (EPR).The results showed that nano ZnO the production of ·OH in the liver of Cipangopaludina Cahayensis; SOD activity and MDA content changed in a similar fashion in that they initially rose then dropped with the concentration of nano ZnO. CAT activity was induced significantly but the change was slow;GST activity remained inhibited. ·OH signal strength was consistent with the change of SOD activity and MDA contents. The activation rate for SOD and MDA was high and CAT and GST activity remained steady.
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Key words:
- nano ZnO /
- free radicals /
- antioxidant system /
- Cipangopaludina cathayensis
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