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李思倩, 路立, 王芬, 杜晓娜, 季民. 低温反硝化过程中pH对亚硝酸盐积累的影响[J]. 环境化学, 2016, 35(8): 1657-1662. doi: 10.7524/j.issn.0254-6108.2016.08.2016011105
引用本文: 李思倩, 路立, 王芬, 杜晓娜, 季民. 低温反硝化过程中pH对亚硝酸盐积累的影响[J]. 环境化学, 2016, 35(8): 1657-1662. doi: 10.7524/j.issn.0254-6108.2016.08.2016011105
shu
LI Siqian, LU Li, WANG Fen, DU Xiaona, JI Min. Effect of pH on nitrite accumulation during denitrification at low temperature[J]. Environmental Chemistry, 2016, 35(8): 1657-1662. doi: 10.7524/j.issn.0254-6108.2016.08.2016011105
Citation: LI Siqian, LU Li, WANG Fen, DU Xiaona, JI Min. Effect of pH on nitrite accumulation during denitrification at low temperature[J]. Environmental Chemistry, 2016, 35(8): 1657-1662. doi: 10.7524/j.issn.0254-6108.2016.08.2016011105
shu

低温反硝化过程中pH对亚硝酸盐积累的影响

  • 1.

    天津大学, 环境科学与工程学院, 天津, 300072;

  • 2.

    天津市城市规划设计研究院, 天津, 300201

  • 基金项目:

    国家自然科学基金-青年基金（51008206）资助.

Effect of pH on nitrite accumulation during denitrification at low temperature

  • 1.

    School of Environment Science and Engineering, Tianjin University, Tianjin, 300072, China;

  • 2.

    Tianjin Urban Planning and Design Institute, Tianjin, 300201, China

  • Fund Project: Supported by the National Natural Science Foundation of China (51008206).

  • 摘要: 以乙酸钠为单一碳源，在C/N比为3、温度为12-14℃的低C/N比、低温条件下通过间歇试验研究pH对反硝化亚硝酸盐积累规律影响.试验结果发现，在C/N为3时，pH值在7-9的范围内都能形成亚硝酸盐积累.反应时间为60 min时，亚硝酸盐积累率最大可达50%左右.低温SBR反应器连续试验研究表明，pH值为7.4±0.1和pH值为8.4±0.1时，序批式活性污泥法（SBR）出水亚硝酸盐积累率分别为51%和48%，取pH值为7.4±0.1条件下SBR反应器活性污泥进行酶活力测定发现，硝酸盐还原酶活力是亚硝酸盐还原酶活力的1.2倍，硝酸盐还原酶活力与亚硝酸盐还原酶活力对外界条件变化敏感程度不同，亚硝酸盐还原酶对外界条件变化更为敏感，其受到的抑制作用要大于硝酸盐还原酶，进而造成NO2--N的积累.
    Abstract: Effect of pH on nitrite accumulation during denitrification was investigated by batch experiments at low C/N ratio of 3 and low temperature of 12-14℃ using acetate as the sole carbon source. The results showed that nitrite accumulated when C/N ratio was 3 and the initial pH was between 7 and 9. The nitrite accumulation rate was about 50% when the reaction time was 60 min. The continuous SBR(Sequencing batch reactor activated sludge process) test at low temperatures showed that when pH was 7.4±0.1and 8.4±0.1, the nitrite accumulation rate were 51% and 48%, respectively. The denitrification sludge in SBR, which operated under pH of 7.4±0.1, was taken for enzyme activity test. It was found that nitrate reductase activity was 1.2 times as high as nitrite reductase activity. The sensitivity of nitrate reductase activity and nitrite reductase activity to the changes of external condition was different, and nitrite reductase activity was more sensitive to the external condition. The inhibition on nitrite reductase activity was more than nitrate reductase, which resulted in nitrite accumulation.
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  • 收稿日期:  2016-01-11
  • 刊出日期:  2016-08-15
李思倩, 路立, 王芬, 杜晓娜, 季民. 低温反硝化过程中pH对亚硝酸盐积累的影响[J]. 环境化学, 2016, 35(8): 1657-1662. doi: 10.7524/j.issn.0254-6108.2016.08.2016011105
引用本文: 李思倩, 路立, 王芬, 杜晓娜, 季民. 低温反硝化过程中pH对亚硝酸盐积累的影响[J]. 环境化学, 2016, 35(8): 1657-1662. doi: 10.7524/j.issn.0254-6108.2016.08.2016011105
shu
LI Siqian, LU Li, WANG Fen, DU Xiaona, JI Min. Effect of pH on nitrite accumulation during denitrification at low temperature[J]. Environmental Chemistry, 2016, 35(8): 1657-1662. doi: 10.7524/j.issn.0254-6108.2016.08.2016011105
Citation: LI Siqian, LU Li, WANG Fen, DU Xiaona, JI Min. Effect of pH on nitrite accumulation during denitrification at low temperature[J]. Environmental Chemistry, 2016, 35(8): 1657-1662. doi: 10.7524/j.issn.0254-6108.2016.08.2016011105
shu

低温反硝化过程中pH对亚硝酸盐积累的影响

  • 1.  天津大学, 环境科学与工程学院, 天津, 300072;
  • 2.  天津市城市规划设计研究院, 天津, 300201
  • 收稿日期:  2016-01-11
基金项目:

国家自然科学基金-青年基金（51008206）资助.

摘要: 以乙酸钠为单一碳源，在C/N比为3、温度为12-14℃的低C/N比、低温条件下通过间歇试验研究pH对反硝化亚硝酸盐积累规律影响.试验结果发现，在C/N为3时，pH值在7-9的范围内都能形成亚硝酸盐积累.反应时间为60 min时，亚硝酸盐积累率最大可达50%左右.低温SBR反应器连续试验研究表明，pH值为7.4±0.1和pH值为8.4±0.1时，序批式活性污泥法（SBR）出水亚硝酸盐积累率分别为51%和48%，取pH值为7.4±0.1条件下SBR反应器活性污泥进行酶活力测定发现，硝酸盐还原酶活力是亚硝酸盐还原酶活力的1.2倍，硝酸盐还原酶活力与亚硝酸盐还原酶活力对外界条件变化敏感程度不同，亚硝酸盐还原酶对外界条件变化更为敏感，其受到的抑制作用要大于硝酸盐还原酶，进而造成NO2--N的积累.

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