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六溴环十二烷(HBCD)对人肝癌细胞HepG2的细胞毒性及机制

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向明钧, 陈玲, 李宇丹, 张钰华, 周佩云, 刘铁军, 孙远鹏, 伍梦娟, 方星薪, 杨超萍. 六溴环十二烷(HBCD)对人肝癌细胞HepG2的细胞毒性及机制[J]. 环境化学, 2016, 35(2): 311-316. doi: 10.7524/j.issn.0254-6108.2016.02.2015081003
引用本文: 向明钧, 陈玲, 李宇丹, 张钰华, 周佩云, 刘铁军, 孙远鹏, 伍梦娟, 方星薪, 杨超萍. 六溴环十二烷(HBCD)对人肝癌细胞HepG2的细胞毒性及机制[J]. 环境化学, 2016, 35(2): 311-316. doi: 10.7524/j.issn.0254-6108.2016.02.2015081003
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XIANG Mingjun, CHEN Ling, LI Yudan, ZHANG Yuhua, ZHOU Peiyun, LIU Tiejun, SUN Yuanpeng, WU Mengjuan, FANG Xingxin, YANG Chaoping. The cytotoxicity and mechanism of Hexabromoeyelododecane(HBCD) on HepG2 cells[J]. Environmental Chemistry, 2016, 35(2): 311-316. doi: 10.7524/j.issn.0254-6108.2016.02.2015081003
Citation: XIANG Mingjun, CHEN Ling, LI Yudan, ZHANG Yuhua, ZHOU Peiyun, LIU Tiejun, SUN Yuanpeng, WU Mengjuan, FANG Xingxin, YANG Chaoping. The cytotoxicity and mechanism of Hexabromoeyelododecane(HBCD) on HepG2 cells[J]. Environmental Chemistry, 2016, 35(2): 311-316. doi: 10.7524/j.issn.0254-6108.2016.02.2015081003
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六溴环十二烷(HBCD)对人肝癌细胞HepG2的细胞毒性及机制

  • 1.

    吉首大学医学院;

  • 2.

    吉首大学生物资源与环境科学学院, 吉首, 416000

  • 基金项目:

    吉首大学2013年大学生研究性学习与创新性实验计划项目(JSU-CX-2013-32)

    国家自然科学基金(81360397)资助.

The cytotoxicity and mechanism of Hexabromoeyelododecane(HBCD) on HepG2 cells

  • 1.

    College of Medical Science;

  • 2.

    College of Biology and Environmental Sciences, Jishou University, Jishou, 416000, China

  • Fund Project: Supported by the Students' Research Learning and Innovative Experimental Project of Jishou University(JSU-CX-2013-32) and the National Natural Science Foundation of China(81360397).

  • 摘要: 为研究六溴环十二烷(Hexabromoeyelododecane, HBCD)对人肝癌细胞HepG2的毒性效应及机制,设3个不同剂量HBCD试验组(10、15、20μg·mL-1)和溶剂对照组、阴性对照组及阳性对照组,将HepG2细胞处理24、48、72 h后,用MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide)法检测细胞毒性;24 h后采用胞质分裂阻断法测定微核率;另在24 h后用细胞内2,7-二氢二氯荧光素(2',7'-dichlorofluorescin diacetate, DCFH-DA)法测定细胞内活性氧(Reactive oxygen species, ROS)的浓度.实验显示,HBCD对HepG2细胞有毒性效应,且存在一定的时间和剂量效应,并导致HepG2细胞的微核率和活性氧的浓度增高.研究表明,HBCD对HepG2细胞有明显的细胞毒性.
    Abstract: For understanding the mechanism and the cytotoxicity of HBCD(Hexabromoeyelododecane)on HepG2 cells, we used the MTT assay,a cytokinesis-block micronucleus assay to evaluate the cytotoxicity of HBCD(10,15,20μg·mL-1) on HepG2 cells as well as the control groups, and measured the fluorescent intensity of reactive oxygen species(ROS) using DCFH-DA. The results showed that the higher concentrations of HBCD reduced the cell survival rates. There were significant differences in the frequency of cytokinesis-block micronucleus between the groups. A significant increased frequency of cytokinesis-block micronucleus was observed in 10,15,20μg·mL-1 dose group compared with the negative control group(PPPin vitro.
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  • [1] MORF L S, TREMP J, GLOOR R, et al. Brominated flame retardants in waste electrical and electronic equipment:Substance flows in a recycling plant[J]. Environ Sci Technol, 2005, 39(22):8691-8699.
    [2] TUE N M, TAKAHASHI S, SUZUKI G, et al. Contamination of indoor dust and air by polychlorinated biphenyls and brominated flame retardants and relevance of non-dietary exposure in Vietnamese informal e-waste recycling sites[J]. Environ Int, 2013, 51:160-167.
    [3] 胡小钟,徐盈,胡德聪.六溴环十二烷异构体在鮰鱼体内的浓度分布与生物累积特征[J].分析科学学报,2008,24(2):125-129.

    HU X Z, XU Y, HU D C. Distribution and biomagnification characteristics of hexabromocyclododecanes(HBCDs) in channel catfish[J]. Journal of Analytical Science, 2008, 24(2):125-129(in Chinese).

    [4] ZHU L Y,MA B L,RONALDA H. Brominated flame retardants in serum from the general population in Northern China[J]. Environ Sci Technol, 2009, 43(18):6963-6968.
    [5] THOMSEN C, STIGUM H, FRØSHAUG M, et al. Determinants of brominated flame retardants in breast milk from a large scale Norwegian study[J]. Environ Int, 2010, 36(1):68-74.
    [6] ELJARRAT E, GUERRA P, MARTÍNEZ E, et al. Hexabromocyclododecane in humanbreast milk:Levels and enantiomeric patterns[J]. Environ Sci Technol, 2009, 43(6):1940-1946.
    [7] KALACHOVA K, HRADKOVA P, LANKOVA D, et al. Occurrence of brominated flame retardants in household and car dust from the Czech Republic[J]. Science of the Total Environment, 2012, 441:182-193.
    [8] SHI Z, JIAO Y, HU Y, et al. Levels of tetrabromobisphenol A, hexabromocyclododecanes and polybrominated diphenyl ethers in human milk from the general population in Beijing, China[J]. Science of the Total Environment, 2013, 452-453:10-18.
    [9] SAEGUSA Y, FUJIMOTO H, WOO G H, et al. Developmental toxicity of brominated flame retardants, tetrabromobisphenol A and 1,2,5,6,9,10-hexabromocyclododecane in rat offspring after maternal exposure rom mid-gestation through lactation[J]. Reproductive Toxicology, 2009, 28(4):456-457.
    [10] PALACE V P, PLESKACH K, HALLDORSON T, et al. Biotransformation enzymes and thyroid axis disruption in juvenile rainbow trout(Oncorhynchus mykiss) exposed to hexabromocyclododecane diastereoisomers[J]. Environ Sci Technol, 2008, 42(6):1967-1972.
    [11] 王晓蓉, 苏燕, 罗义. HBCD诱导鲫鱼ROS和HSP70的产生及分子致毒机制[C].北京:第二届持久性有机污染物全国学术研讨会论文集,2007:111-112. WANG X Y, SU Y, LUO Y. Generation and molecular toxic mechanism of ROS and HSP70 induced by HBCD in carp[C]. Beijing:The collected papers of second national symposium on persistent organic pollutants(POPs), 2007

    :111-112(in Chinese).

    [12] 吴艳娣.几种典型持久性有机污染物对斑马鱼胚胎发育的毒性效应研究[D]. 武汉华中农业大学硕士学位论文,2008. WU Y D. Study on the toxicity of several typical POPs exposed to zebrafish(Danio Rerio) embryos[D]. Wuhan:Master's degree dissertation of HuaZhong Agricultural University, 2008(in Chinese).
    [13] FERY Y, BUSCHAUER I, SALZIG C, et al. Technical pentabromodipheny ether and exabromocyclododecane as activators of the pregnane-X-receptor(PXR)[J]. Toxicology, 2009, 264(1-2):45-51.
    [14] 李永东,云霞,那广水,等. 环境中六溴环十二烷的研究进展[J]. 环境与健康杂志, 2010, 27(10):933-935.

    LI Y D,YUN X, NA G S, et al. Progress in research on the Hexabromoeyelododeeane in environment[J]. J Environ Health, 2010, 27(10):933-935(in Chinese).

    [15] Natasha C H,Margaret M W. Hexabromocyclododecane decreases the lytic function and ATP levels of human natural killer cells[J]. Journal of Applied Toxicol, 2009, 29(8):656-661.
    [16] Sorsa M, Wilbourn J, Vainio H. Human cytogenetic damage as a predictor of cancer risk:Mechanisms of carcinogenesis in risk identification[C]. IARC Scientific Publications, 1992:543-554.
    [17] MARIUSSEN E, FONNUM F. The effect of brominated flame retardants on neurotransmitter uptake into rat brain synaptosomes and vesicles[J]. Neurochem Int, 2003,43(4-5):533-542.
    [18] 刘艳平, 张树兵, 杨粤军,等. 生物学实验指导[M]. 长沙:湖南科学技术出版社, 2013:74-75. LIU Y P, ZHANG S B, YANG Y J, et al. Biological experimental instruction[M]. Changsha:Hunan Science and Technology Press, 2013:74

    -75(in Chinese).

    [19] 徐八一,何卫红,何平,等. PBDE_47对人神经母细胞瘤SH_SY5Y细胞致突变作用[J]. 癌变·畸变·突变, 2007, 19(6):457-459.

    XU B Y, HE W H, HE P, et al. Assessment of mutagenicity of PBDE_47 in human neurobla stoma SH_SYSY Cells in Vitro[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2007, 19(6):457-459(in Chinese).

    [20] 邹云锋,牛丕业,宫智勇,等.活性氧在硒致HepG2 DNA损伤中的作用[J].卫生研究, 2006, 35(3):291-293.

    ZOU Y F, NIU P Y, GONG Z Y, et al. Role of reactive oxygen species in sodium selenite induced DNA damage in HepG2 cells[J]. Journal of Hygiene Research, 2006, 35(3):291-293(in Chinese).

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  • 收稿日期:  2015-08-10
  • 刊出日期:  2016-02-15

六溴环十二烷(HBCD)对人肝癌细胞HepG2的细胞毒性及机制

  • 1.  吉首大学医学院;
  • 2.  吉首大学生物资源与环境科学学院, 吉首, 416000
  • 收稿日期:  2015-08-10
基金项目:

吉首大学2013年大学生研究性学习与创新性实验计划项目(JSU-CX-2013-32)

国家自然科学基金(81360397)资助.

摘要: 为研究六溴环十二烷(Hexabromoeyelododecane, HBCD)对人肝癌细胞HepG2的毒性效应及机制,设3个不同剂量HBCD试验组(10、15、20μg·mL-1)和溶剂对照组、阴性对照组及阳性对照组,将HepG2细胞处理24、48、72 h后,用MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide)法检测细胞毒性;24 h后采用胞质分裂阻断法测定微核率;另在24 h后用细胞内2,7-二氢二氯荧光素(2',7'-dichlorofluorescin diacetate, DCFH-DA)法测定细胞内活性氧(Reactive oxygen species, ROS)的浓度.实验显示,HBCD对HepG2细胞有毒性效应,且存在一定的时间和剂量效应,并导致HepG2细胞的微核率和活性氧的浓度增高.研究表明,HBCD对HepG2细胞有明显的细胞毒性.

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