人体胆汁中微塑料的检测与分析
Detection and Analysis of Microplastics in Human Bile
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摘要: 微塑料(MPs)因其广泛分布及其对自然生态系统的影响而受到越来越多关注，但迄今为止还没有研究报告微塑料在人体胆汁中的暴露，本研究通过对人体胆汁中的微塑料进行定性和定量分析，以期为今后深入探究微塑料与人类疾病的关系提供依据。纳入武汉科技大学附属孝感医院2023年3月—5月确诊为胆囊结石并行腹腔镜下胆囊切除术患者12例，收集患者的胆汁样本作为实验组，以程序空白、实验空白及仪器空白作为对照组，采用热解-气相色谱/质谱(Py-GC/MS)方法检测2组样本中微塑料的种类及含量。在12个胆汁样本中检测到聚氯乙烯(PVC)、聚对苯二甲酸乙二醇酯(PET)、尼龙66(PA66)和聚乙烯(PE)共4种微塑料类型，在单个样本中可检测到1~3种不同的微塑料种类，检测结果阳性率为100%(12/12)，其中PVC阳性率为100%(12/12)，PA66阳性率为91.66%(11/12)，PET阳性率为25.0%(3/12)，PE阳性率为8.33%(1/12)。在检测到该塑料颗粒的样本中，PA66含量为(10.36±8.62) μg·g-1，PVC平均含量为14.53 μg·g-1，PET平均含量为10.66 μg·g-1，在唯一检测到PE的样本中，其含量为3.01 μg·g-1。本研究对人体胆汁中的微塑料进行定性和定量分析，为微塑料与人类疾病的深入探讨提供依据。
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关键词:
- 微塑料 /
- 人体 /
- 胆汁 /
- 热解-气相色谱/质谱
Abstract: Microplastics (MPs) have attracted increasing attention due to their wide distribution and impact on natural ecosystems. However, there have been no reports on the exposure of microplastics in human bile to date. This study conducted qualitative and quantitative analysis of microplastics in human bile, in order to provide a basis for further exploration of the relationship between microplastics and human diseases. 12 patients diagnosed with gallstones and underwent laparoscopic cholecystectomy in Xiaogan Hospital, which is affiliated to Wuhan University of Science and Technology, were included from March to May 2023. The bile samples from those patients were collected as the experimental group, while the procedure blanks, experimental blanks and instrument blanks were used as control groups. Pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) method was employed to detect the types and amounts of microplastics in the two groups of samples. Four types of microplastics, polyvinyl chloride (PVC), polyethylene terephthalate (PET), nylon 66 (PA66), and polyethylene (PE), were detected in 12 bile samples. One to three different types of microplastics were detected in individual samples, and the positive rate was 100% (12/12). The positive rate of PVC, PA66, PET, and PE were 100% (12/12), 91.66% (11/12), 25.0% (3/12), and 8.33% (1/12), respectively. In the samples where the plastic particles were detected, the content of PA66 was (10.36±8.62) μg·g-1, the average content of PVC was 14.53 μg·g-1, and the average content of PET was 10.66 μg·g-1. In the sole sample where PE was detected, the content was 3.01 μg·g-1. In this study, the qualitative and quantitative analysis of microplastics in human bile was conducted to provide a basis for the further exploration of relationship between microplastics and human diseases.-
Key words:
- microplastics /
- human /
- bile /
- Py-GC/MS
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Hirt N, Body-Malapel M. Immunotoxicity and intestinal effects of nano- and microplastics: A review of the literature [J]. Particle and Fibre Toxicology, 2020, 17(1): 57 王一飞, 李淼, 于海瀛, 等. 微塑料对环境中有机污染物吸附解吸的研究进展[J]. 生态毒理学报, 2019, 14(4): 23-30 Wang Y F, Li M, Yu H Y, et al. Research progress on the adsorption and desorption between microplastics and environmental organic pollutants [J]. Asian Journal of Ecotoxicology, 2019, 14(4): 23-30 (in Chinese)
Lambert S, Scherer C, Wagner M. Ecotoxicity testing of microplastics: Considering the heterogeneity of physicochemical properties [J]. Integrated Environmental Assessment and Management, 2017, 13(3): 470-475 Stapleton M J, Hai F I. Microplastics as an emerging contaminant of concern to our environment: A brief overview of the sources and implications [J]. Bioengineered, 2023, 14(1): 2244754 Rhodes C J. Solving the plastic problem: From cradle to grave, to reincarnation [J]. Science Progress, 2019, 102(3): 218-248 Bhagat J, Zang L Q, Nishimura N, et al. Zebrafish: An emerging model to study microplastic and nanoplastic toxicity [J]. The Science of the Total Environment, 2020, 728: 138707 Sözener Z C, Cevhertas L, Nadeau K, et al. Environmental factors in epithelial barrier dysfunction [J]. The Journal of Allergy and Clinical Immunology, 2020, 145(6): 1517-1528 Hwang J, Choi D, Han S, et al. Potential toxicity of polystyrene microplastic particles [J]. Scientific Reports, 2020, 10(1): 7391 Yang X, Man Y B, Wong M H, et al. Environmental health impacts of microplastics exposure on structural organization levels in the human body [J]. The Science of the Total Environment, 2022, 825: 154025 梁競文, 王丽娜. 肠脑轴中胆汁酸功能研究进展[J]. 中国畜牧兽医, 2023, 50(3): 924-931 Liang J W, Wang L N. Research progress of bile acid function in gut-brain axis [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(3): 924-931 (in Chinese)
钟浩, 王俭, 冯凤琴, 等. 高脂膳食、肠道菌群和胆汁酸代谢与胰岛素抵抗之间关系的研究进展[J]. 中国食品学报, 2023, 9(3): 347-364 Zhong H, Wang J, Feng F Q, et al. Research progress on the relationship between high-fat diet, intestinal flora, bile acid metabolism and insulin resistance [J]. Journal of Chinese Institute of Food Science and Technology, 2023, 9(3): 347-364 (in Chinese)
刘琳琳, 金墨竹, 窦德强. 基于UPLC-QqQ-MS/MS探究人参属中药对甲亢大鼠胆汁酸成分代谢影响[J]. 质谱学报, 2023, 44(4): 567-575 Liu L L, Jin M Z, Dou D Q. UPLC-QqQ-MS/MS-based quantitative analysis on bile acid in hyperthyroidism rats to explore the effects of ginseng herbs [J]. Journal of Chinese Mass Spectrometry Society, 2023, 44(4): 567-575 (in Chinese)
Schwabl P, Köppel S, Königshofer P, et al. Detection of various microplastics in human stool: A prospective case series [J]. Annals of Internal Medicine, 2019, 171(7): 453-457 Liu S J, Guo J L, Liu X Y, et al. Detection of various microplastics in placentas, meconium, infant feces, breastmilk and infant formula: A pilot prospective study [J]. The Science of the Total Environment, 2023, 854: 158699 Leslie H A, van Velzen M J M, Brandsma S H, et al. Discovery and quantification of plastic particle pollution in human blood [J]. Environment International, 2022, 163: 107199 Jenner L C, Rotchell J M, Bennett R T, et al. Detection of microplastics in human lung tissue using μFTIR spectroscopy [J]. Science of the Total Environment, 2022, 831: 154907 Abbasi S, Turner A. Human exposure to microplastics: A study in Iran [J]. Journal of Hazardous Materials, 2021, 403: 123799 Horvatits T, Tamminga M, Liu B B, et al. Microplastics detected in cirrhotic liver tissue [J]. EBioMedicine, 2022, 82: 104147 Ibrahim Y S, Tuan Anuar S, Azmi A A, et al. Detection of microplastics in human colectomy specimens [J]. JGH Open: an Open Access Journal of Gastroenterology and Hepatology, 2021, 5(1): 116-121 Brander S M, Renick V C, Foley M M, et al. Sampling and quality assurance and quality control: A guide for scientists investigating the occurrence of microplastics across matrices [J]. Applied Spectroscopy, 2020, 74(9): 1099-1125 Kadac-Czapska K, Knez E, Grembecka M. Food and human safety: The impact of microplastics [J]. Critical Reviews in Food Science and Nutrition, 2024, 64(11): 3502-3521 Senathirajah K, Attwood S, Bhagwat G, et al. Estimation of the mass of microplastics ingested: A pivotal first step towards human health risk assessment [J]. Journal of Hazardous Materials, 2021, 404(Pt B): 124004 Kwon J H, Kim J W, Pham T D, et al. Microplastics in food: A review on analytical methods and challenges [J]. International Journal of Environmental Research and Public Health, 2020, 17(18): 6710 Bonanomi M, Salmistraro N, Porro D, et al. Polystyrene micro and nano-particles induce metabolic rewiring in normal human colon cells: A risk factor for human health [J]. Chemosphere, 2022, 303(Pt 1): 134947 Kumar R, Manna C, Padha S, et al. Micro(nano) plastics pollution and human health: How plastics can induce carcinogenesis to humans? [J]. Chemosphere, 2022, 298: 134267 Yuan Z H, Nag R, Cummins E. Human health concerns regarding microplastics in the aquatic environment: From marine to food systems [J]. The Science of the Total Environment, 2022, 823: 153730 Chen X B, Zhuang J S, Chen Q L, et al. Chronic exposure to polyvinyl chloride microplastics induces liver injury and gut microbiota dysbiosis based on the integration of liver transcriptome profiles and full-length 16S rRNA sequencing data [J]. The Science of the Total Environment, 2022, 839: 155984 贺雨田. 微塑料检测方法的研究[D]. 北京: 北京化工大学, 2021: 9-12 He Y T. Research on detection methods of microplastics [D]. Beijing: Beijing University of Chemical Technology, 2021: 9 -12 (in Chinese)
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