锌与双酚A复合暴露对普通小球藻的联合毒性作用评估
Joint Toxicity of Zinc and Bisphenol A on Chlorella vulgaris
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摘要: 为探究重金属与环境激素的联合毒性效应，以普通小球藻为受试生物，开展了锌与双酚A的单一及复合暴露对普通小球藻的急性毒性、叶绿素含量、可溶性蛋白含量及抗氧化应激的影响。结果显示，在单一暴露条件下，锌和双酚A对普通小球藻的EC50分别为5.10 mg·L-1和17.37 mg·L-1，仅在锌离子质量浓度低于0.25 mg·L-1时可促进普通小球藻叶绿素合成，而其他各暴露组中锌和双酚A对普通小球藻的光合作用具有抑制效应，采用等毒性配比法进行联合毒性试验，由相加指数法判断锌与双酚A对普通小球藻的联合作用类型为协同作用；藻细胞可溶性蛋白含量、抗氧化酶活性及丙二醛含量随锌与双酚A暴露浓度的增加被显著诱导，且高浓度的复合暴露对普通小球藻造成明显的氧化损伤。研究表明，锌与双酚A复合暴露会加强其在水环境中的毒性效应。Abstract: In order to evaluate the joint toxicity of heavy metals and environmental hormones, Chlorella vulgaris was selected as the sample organism to test and analyze the acute toxicity, the effect on chlorophyll content, soluble protein content and anti-oxidative stress, which was exposed on zinc (Zn, sample heavy metal) and bisphenol A (BPA, endocrine disturbing chemical) separately and combinedly. Results demonstrated that the 7 d-EC50 of Zn and BPA to Chlorella vulgaris was 5.10 mg·L-1 and 17.37 mg·L-1, respectively. The chlorophyll synthesis of Chlorella vulgaris was promoted only when the concentration of zinc ion was lower than 0.25 mg·L-1, while the photosynthesis of Chlorella vulgaris was inhibited by Zn and BPA in other concentration situations. The joint toxicity tests of Zn and BPA were carried out by equal toxicity ratio method, and the impact of both on Chlorella vulgaris was identified as synergistic effect by the additive index method. However, soluble protein content, antioxidant enzyme activity and malondialdehyde content in algal cells were significantly induced, and oxidative damage obviously occurred, due to the increasing exposed concentration of Zn and BPA. This study states that the combined exposure of Zn and BPA enhances the joint toxic effects in the aquatic environment.
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Key words:
- zinc /
- bisphenol A /
- Chlorella vulgaris /
- joint toxicity /
- oxidative stress
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