4种多环芳烃对虾夷扇贝(Patinopecten yessoensis)幼体的急性毒性研究
Acute Toxicity of Four PAHs to Larva of Patinopecten yessoensis
-
摘要: 为探究苯并芘、三甲基菲、二甲基蒽和惹烯对海洋生物的毒性作用，以虾夷扇贝（Patinopecten yessoensis）为受试生物，采用半静水式实验方式，探讨了4种多环芳烃（PAHs）对虾夷扇贝幼体的毒性效应。在不同浓度的苯并芘、三甲基菲、二甲基蒽和惹烯作用下，观察虾夷扇贝的卵子受精率和幼体死亡率。结果表明，PAHs实验组与对照组相比，卵子的受精率显著降低，幼体死亡率显著增加，与PAHs浓度之间存在剂量-效应关系（P<0.05）。苯并芘对虾夷扇贝幼体在9、24和48 h的半致死浓度（LC50）分别为252.75、115.85和48.13 mg·L-1，二甲基蒽对虾夷扇贝幼体在9、24和48 h的LC50分别为529.57、206.46和68.42 mg·L-1，三甲基菲对虾夷扇贝幼体在9、24和48 h的LC50分别为440.28、290.72和92.40 mg·L-1，惹烯对虾夷扇贝幼体在9、24和48 h的LC50分别为162.92、129.51和63.97 mg·L-1。4种PAHs的安全浓度（SC）分别为2.49、2.25、2.80和4.68 mg·L-1，其毒性大小顺序为苯并芘 > 惹烯 > 二甲基蒽 > 三甲基菲。同时，借助定量构效关系（QSAR）的研究结果对PAHs化合物毒性原理进行了初步讨论。本实验为评估PAHs对海洋生物安全的风险提供了参考依据。Abstract: In order to study the ecotoxicological impact of marine polycyclic aromatic hydrocarbons (PAHs) pollutants on Patinopecten yessoensis, the toxic effects of benzopyrene, 1, 9, 10-trimethylphenanthrene, 9, 10-dimethylanthracene and retene on larva of Patinopecten yessoensis were investigated under semi-static conditions. The effects of benzopyrene, 1, 9, 10-trimethylphenanthrene, 9, 10-dimethylanthracene and retene on the ovum fertilization rate and infant mortality were observed in different concentrations. The results showed that compared with the control group, the ovum fertilization rate and the infant mortality in the treatment groups with high concentrations of PAHs was significantly decreased, which had a significant dose-effect relationship with the treatment concentration (P<0.05). The median lethal concentration (LC50) of benzopyrene on infant of Patinopecten yessoensis at 9, 24 and 48 h was 252.75, 115.85 and 48.13 mg·L-1. The LC50 of 9, 10-dimethylanthracene was 529.57, 206.46 and 68.42 mg·L-1 at 9, 24 and 48 h; the LC50 of 1, 9, 10-trimethylphenanthrene was 440.28, 290.72 and 92.40 mg·L-1 at 9, 24 and 48 h; the LC50 of retene was 162.92, 129.51 and 63.97 mg·L-1 at 9, 24 and 48 h, respectively. The safe concentrations (SC) of four PAHs were 2.49, 2.25, 2.80 and 4.68 mg·L-1, respectively. The toxicity of four PAHs followed as benzopyrene > retene > 9, 10-dimethylanthracene > 1, 9, 10-trimethylphenanthrene. In this paper, the mechanism of PAHs toxicity was discussed by quantitative structure-activity relationship (QSAR). This experiment provides the scientific data for predicting the risk of PAHs to marine biological safety.
-
Key words:
- PAHs /
- Patinopecten yessoensis /
- toxic effect /
- LC50 /
- QSAR
-
-
Engraff M, Solere C, Smith K E C, et al. Aquatic toxicity of PAHs and PAH mixtures at saturation to benthic amphipods:Linking toxic effects to chemical activity[J]. Aquatic Toxicology, 2011, 102(3-4):140-149 李香, 魏海峰, 刘长发, 等. 2种烷基多环芳烃对仿刺参CYP450和p53基因表达的影响研究[J]. 生态毒理学报, 2019, 14(1):83-89 Li X, Wei H F, Liu C F, et al. Effects of 2 kinds of alkyl-PAHs on the expression of CYP450 and P53 genes of Apostichopus japonicus[J]. Asian Journal of Ecotoxicology, 2019, 14(1):83-89(in Chinese)
李晓静, 于玉玲, 马莉, 等. 太湖胥口湾表层水和沉积物中多环芳烃的浓度水平及生态风险[J]. 生态毒理学报, 2016, 11(2):547-555 Li X J, Yu Y L, Ma L, et al. The contamination levels of polycyclic aromatic hydrocarbons in surface water and sediments in Xukou Bay of Taihu Lake and the associated ecological risk[J]. Asian Journal of Ecotoxicology, 2016, 11(2):547-555(in Chinese)
邓伟. 南黄海、东海表层沉积物中脂肪烃与多环芳烃的分布特征及来源初步研究[D]. 青岛:中国海洋大学, 2013:4-92 Deng W. A preliminary study on the composition, distribution and source apportionment of aliphatic and polycyclic aromatic hydrocarbons in surface sediments from the South Yellow Sea and East China Sea[D]. Qingdao:Ocean University of China, 2013:4 -92(in Chinese)
Li J, Shang X, Zhao Z, et al. Polycyclic aromatic hydrocarbons in water, sediment, soil, and plants of the Aojiang River waterway in Wenzhou, China[J]. Journal of Hazardous Materials, 2010, 173(1-3):75-81 王萌, 雷忻, 田鹏飞, 等. 多环芳烃对水生生物生殖毒性的研究进展[J]. 延安大学学报:自然科学版, 2017, 36(2):98-101 Wang M, Lei X, Tian P F, et al. The research progress of reproductive toxicity of polycyclic aromatic hydrocarbons on aquatic organisms[J]. Journal of Yanan University:Natural Science Edition, 2017, 36(2):98-101(in Chinese)
高振锟. 环境胁迫对虾夷扇贝生理、免疫指标及行为学特性的影响[D]. 上海:上海海洋大学, 2016:2-7 Gao Z K. Effects of environmental stresses on physiological, immunological parameters and behavioral characteristics of Patinopecten yessoensis[D]. Shanghai:Shanghai Ocean University, 2016:2 -7(in Chinese)
孟晓林. 铜胁迫条件下虾夷扇贝鳃组织的转录组应答[C]//中国水产学会动物营养与饲料专业委员会. 第九届世界华人鱼虾营养学术研讨会论文摘要集. 厦门:中国水产学会动物营养与饲料专业委员会, 2013:271 Meng X L. Study of the transcriptomic response to copper exposure by the gill tissue of Japanese scallops (Mizuhopecten yessoensis) using deep-sequencing technology[C]//The Animal Nutrition and Feed Professional Committee, The China Society of Fisheries. The 10th Symposium of World's Chinese Scientists on Nutrition and Feeding of Finfish and Shelffish. Xiamen:The Animal Nutrition and Feed Professional Committee, The China Society of Fisheries, 2013:271 (in Chinese)
关晓燕, 王摆, 董颖, 等. 二甲苯、蒽、苯并芘对虾夷扇贝幼贝血清SOD、CAT活性的影响[J]. 生态毒理学报, 2016, 11(1):289-294 Guan X Y, Wang B, Dong Y, et al. Effects of xylene, antracene, benzopyrene on serum SOD and CAT activity in young Patinopecten yessoensis[J]. Asian Journal of Ecotoxicology, 2016, 11(1):289-294(in Chinese)
段萃枝, 杨凤, 木扎合买提, 等. 亚硝酸态氮对虾夷扇贝早期存活和生长的影响[J]. 大连海洋大学学报, 2019, 34(2):214-219 Duan C Z, Yang F, Muzha H M, et al. Effects of nitrite nitrogen on early survival and growth of yesso scallop Patinopecten yessoensis[J]. Journal of Dalian Ocean University, 2019, 34(2):214-219(in Chinese)
王摆, 贺凌, 高杉, 等. 6种苯系物对虾夷扇贝的生殖毒性作用[J]. 生态毒理学报, 2014, 9(6):1083-1090 Wang B, He L, Gao S, et al. The reproductive toxicities of six BTEXs to Japanese scallop (Mizuhopecten yessoensis)[J]. Asian Journal of Ecotoxicology, 2014, 9(6):1083-1090(in Chinese)
崔正国, 苑旭洲, 崔毅, 等. 虾夷扇贝(Patinopecten yessoensis)对铅和镉的生物富集与释放规律[J]. 渔业科学进展, 2015, 36(3):116-124 Cui Z G, Yuan X Z, Cui Y, et al. Biological enrichment and release of lead and cadmium from Patinopecten yessoensis[J]. Progress in Fishery Sciences, 2015, 36(3):116-124(in Chinese)
孟晓林. 镉、铜分别胁迫虾夷扇贝的毒性作用机制研究[D]. 北京:中国科学院大学, 2013:5-99 Meng X L. Study on the mechanism of toxicity of cadmium and copper to Patinopecten yessoensis[D]. Beijing:University of Chinese Academy of Sciences, 2013:5 -99(in Chinese)
赵玉明, 田相利. 底栖硅藻种类组成对皱纹盘鲍匍匐幼体和稚鲍生长、存活的影响[J].水产科学, 2011, 30(3):144-147 Zhao Y M, Tian X L. Effects of species composition of benthic diatoms on growth and survival of postlarval and juvenile abalone Haliotis discus hannai[J]. Fisheries Science, 2011, 30(3):144-147(in Chinese)
徐杭忠, 刘琴琴, 唐毅. 4种水产药物对长吻鱼危稚鱼的急性毒性试验[J]. 水产科技情报, 2020, 47(1):10-13 ,17 Xu H Z, Liu Q Q, Tang Y. Acute toxicity test of four aquatic drugs on juvenile fish[J]. Fisheries Science and Technology Information, 2020, 47(1):10-13,17(in Chinese)
Talebi S M, Mohammad-Khoh A. Vapor and particle phase determination of polycyclic aromatic hydrocarbons in urban air[J]. Bulletin of Environmental Contamination & Toxicology, 2003, 71(5):1042-1047 吴健, 谭娟, 王敏, 等. 某石油污染滩涂沉积物中总石油烃和多环芳烃组成分布特征及源解析[J]. 安全与环境学报, 2016, 16(1):282-287 Wu J, Tan J, Wang M, et al. Analysis for the composition, distribution and sedimental apportionment of a certain oil-polluted tidal marsh source area in terms of TPH and PAH[J]. Journal of Safety and Environment, 2016, 16(1):282-287(in Chinese)
张彤, 宋春诤, 高一楠, 等. 海上溢油事故对海洋生态系统的影响——以"河北精神"号溢油事件为例[J]. 海洋信息, 2015(3):45-49 Zhang T, Song C Z, Gao Y N, et al. Effects of offshore oil spill on marine ecosystem-A case study of the Hebei spirit oil spill[J]. Ocean Information, 2015 (3):45-49(in Chinese)
段美娜, 刘泳江, 白雪, 等. 海底重燃油对海胆繁殖及其子代发育的影响[J]. 中国环境科学, 2018, 38(12):4720-4729 Duan M N, Liu Y J, Bai X, et al. Effects of heavy oil on the reproduction and progeny of sea urchins[J]. China Environmental Science, 2008, 38(12):4720-4729(in Chinese)
董雪纯, 魏海峰, 刘长发. 苯并芘和9,10-二甲基蒽对皱纹盘鲍(Haliotis discus hannai)早期发育的毒性研究[J]. 生态毒理学报, 2019, 14(5):212-218 Dong X C, Wei H F, Liu C F. Toxicity of benzopyrene and 9,10-dimethylanthracene on early development of Haliotis discus hannai[J]. Asian Journal of Ecotoxicology, 2019, 14(5):212-218(in Chinese)
陈漪, 王晓杰, 冉皓宇, 等. 海水模式种青鳉鱼(Oryzias melastigma)的胚胎发育观察[J].海洋与湖沼, 2016, 47(1):71-82 Chen Y, Wang X J, Ran H Y, et al. Observation on the embryonic development of Oryzias melastigma[J]. Oceanologia et Limnologia Sinica, 2016, 47(1):71-82(in Chinese)
丛伟. 多环芳烃(PAHs)对海洋鱼类胚胎发育的毒性效应研究[D]. 厦门:厦门大学, 2007:13-68 Cong W. Study on the toxic effect of polycyclic aromatic hydrocarbons (PAHs) on the embryonic development of marine fish[D]. Xiamen:Xiamen University, 2007:13 -68(in Chinese)
董雪纯, 魏海峰, 刘长发. 4种多环芳烃对仿刺参(Apostichopus japonicus)原肠胚发育的急性毒性研究[J]. 生态毒理学报, 2019, 14(1):161-167 Dong X C, Wei H F, Liu C F. Acute toxicity of four polycyclic aromatic hydrocarbons to the development of Apostichopus japonicus gastrointestinal embryo[J]. Asian Journal of Ecotoxicology, 2019, 14(1):161-167(in Chinese)
黄树辉, 马建腾, 章尼娜, 等. 微囊藻毒素-LR和二甲苯对斑马鱼胚胎的毒性作用[J]. 环境与健康杂志, 2013, 30(4):308-311 Huang S H, Ma J T, Zhang N N, et al. Toxic effects of xylene and microcystin-LR on zebrafish embryos[J]. Journal of Environment and Health, 2013, 30(4):308-311(in Chinese)
滕晓强, 陆宏峰, 郭心月, 等. 二氯苯醌对斑马鱼胚胎发育及超氧化物歧化酶的影响[J].环境科学学报, 2020, 40(7):2659-2664 Teng X Q, Lu H F, Guo X Y, et al. The effects of 2,6-DCBQ on the development and superoxide dismutase of zebrafish embryo[J]. Acta Scientiae Circumstantiae, 2020, 40(7):2659-2664(in Chinese)
王斌, 余刚, 黄俊, 等. QSAR/QSPR在POPs归趋与风险评价中的应用[J]. 化学进展, 2007(10):1612-1619 Wang B, Yu G, Huang J, et al. Application of QSAR/QSPR in fate evaluation and risk assessment of POPs[J]. Chemical Progress, 2007 (10):1612-1619(in Chinese)
刘羽晨. 基于综合毒性作用模式分类构建有机化合物对大型蚤急性毒性QSAR模型[D].大连:大连理工大学, 2015:2-12 Liu Y C. QSAR models for acute toxicity of organic substances to Daphnia magna-Based on a comprehensive mode of action classification[D]. Dalian:Dalian University of Technology, 2015:2 -12(in Chinese)
孙雪峰, 丁君, 张辉, 等. 12种常见农药对海胆胚胎发育的毒性影响[J]. 生态毒理学报, 2009, 4(1):147-151 Sun X F, Ding J, Zhang H, et al. Toxic effects of twelve pesticides on the development of sea urchin embryos[J]. Asian Journal of Ecotoxicology, 2009, 4(1):147-151(in Chinese)
-
点击查看大图
计量
- 文章访问数: 2028
- HTML全文浏览数: 2028
- PDF下载数: 47
- 施引文献: 0
下载:
