3种多环芳烃对海胆细胞色素CYP17A1基因表达的影响
Effects of 3 Kinds of PAHs on the Expression of CYP17A1 Genes of Strongylocentrotus intermedius
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摘要: 为揭示不同种类多环芳烃（PAHs）污染物对中间球海胆（Strongylocentrotus intermedius）的生态毒理效应，研究了不同浓度的苯并（a）芘（1、5和20 μg·L-1）以及2种烷基多环芳烃3-甲基菲（5、10和100 μg·L-1）和2-甲基蒽（5、10和50 μg·L-1）对海胆细胞色素CYP17A1基因表达的影响。结果表明，随着暴露时间的增加，不同浓度的3种多环芳烃对海胆细胞色素CYP17A1基因表达产生不同程度的影响。与对照组相比，暴露14 d后，苯并（a）芘20 μg·L-1处理组对海胆CYP17A1基因的表达表现出抑制作用（P<0.05）；暴露14 d后，3-甲基菲100 μg·L-1处理组对海胆CYP17A1基因的表达表现出诱导作用（P<0.05）；暴露14 d后，2-甲基蒽50 μg·L-1处理组对海胆CYP17A1基因的表达表现出抑制作用（P<0.05）。2-甲基蒽对海胆CYP17A1基因表达的影响比苯并（a）芘、3-甲基菲的影响更为明显。Abstract: In order to study the toxic effects of different polycyclic aromatic hydrocarbons (PAHs) on Strongylocentrotus intermedius, the effects of different concentrations of benzopyrene (1, 5, 20 μg·L-1), 3-methylphenanthrene (5, 10, 100 μg·L-1) and 2-methylanthracene (5, 10, 50 μg·L-1) on the expression of cytochrome CYP17A1 gene were investigated. The results showed that with the increase of exposure time, the three PAHs at different concentrations had different effects on the expression of CYP17A1 gene in Strongylocentrotus intermedius. Compared with control group, after exposure for 14 d, the expression of CYP17A1 gene was inhibited by benzopyrene in 20 μg·L-1 group (P<0.05); after exposure for 14 d, the expression of CYP17A1 gene was induced by 3-methylphenanthrene in 100 μg·L-1 group (P<0.05); after exposure for 14 d, the expression of CYP17A1 gene was inhibited by 2-methylanthracene in 50 μg·L-1 group (P<0.05). The effects of 2-methylanthracene on cytochrome CYP17A1 gene expression of Strongylocentrotus intermedius are more obvious than benzopyrene and 3-methylphenanthrene.
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Key words:
- benzopyrene /
- 3-methylphenanthrene /
- 2-methylanthracene /
- Strongylocentrotus intermedius /
- CYP17A1 /
- gene expression
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