土霉素对斜生栅藻的毒性效应研究
Toxic Effects of Oxytetracycline on Scenedesmus obliquus
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摘要: 土霉素被广泛应用于畜牧养殖业，但由于动物对土霉素的吸收效率较低，其不可避免地进入水环境，对水生生物造成影响。为探究土霉素对淡水藻的毒性作用，选择斜生栅藻为受试生物，通过室内暴露实验，研究不同浓度土霉素（0、1、2、5、10和20 mg·L-1）对斜生栅藻的生物毒性效应。研究结果表明，各浓度土霉素暴露组对斜生栅藻生长均有抑制作用，最高抑制率为44.4%，土霉素对斜生栅藻96 h的半数效应浓度（EC50）、无可见效应浓度（NOEC）和最低可观察效应浓度（LOEC）分别为21.3、2和5 mg·L-1；相同暴露条件下，叶绿素a含量与斜生栅藻生物量表现出同样的变化趋势，与对照组相比，20 mg·L-1浓度组中叶绿素a含量在96 h降低了28.9%。暴露96 h，斜生栅藻超氧化物歧化酶（SOD）活性整体呈现出下降趋势，20 mg·L-1浓度组SOD活性受到显著性抑制（P<0.001），比对照组低38.8%；丙二醛（MDA）含量与活性氧（ROS）受到不同程度诱导，20 mg·L-1浓度组MDA含量为对照组的3.2倍，ROS为对照组的1.2倍；10 mg·L-1和20 mg·L-1土霉素对斜生栅藻造成了一定程度的氧化损伤。Abstract: Oxytetracycline has been widely used in animal husbandry. However, due to the low absorption efficiency of oxytetracycline by animals, it could be discharged into the water environment inevitably, leading to impacts on aquatic organisms. In order to reveal the toxic effect of oxytetracycline on freshwater algae, the indoor exposure experiment was employed to explore the biotoxicity effects of different concentrations of oxytetracycline (0, 1, 2, 5, 10 and 20 mg·L-1) on Scenedesmus obliquus. The results indicated that oxytetracycline at each level of concentration inhibited the growth of Scenedesmus obliquus with the highest inhibition rate of 44.4%. The 96 h 50% effect concentration (96 h-EC50), no observed effect concentration (NOEC) and lowest observed effect concentration (LOEC) of oxytetracycline on Scenedesmus obliquus were 21.3, 2 and 5 mg·L-1, respectively. Under the same exposure conditions, the chlorophyll-a content exhibited the same change trend as the biomass of Scenedesmus obliquus. 20 mg·L-1 of oxytetracycline decreased the chlorophyll content of Scenedesmus obliquus by 28.9% at 96 h. Superoxide dismutase (SOD) activity of Scenedesmus obliquus presented a downward trend after exposure to oxytetracycline for 96 h. The SOD activity was significantly inhibited (P<0.001) by oxytetracycline at the concentration of 20 mg·L-1, with 38.8% of decrease in contrast to the control. Malonaldehyde (MDA) content and reactive oxygen species (ROS) of Scenedesmus obliquus were induced by oxytetracycline differently, i.e., they were increased by 3.2 times and 1.2 times respectively by 20 mg·L-1 of oxytetracycline, in contrast to the control. Both 10 mg·L-1 and 20 mg·L-1 of oxytetracycline caused certain oxidative damage to Scenedesmus obliquus.
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Key words:
- oxytetracycline /
- Scenedesmus obliquus /
- oxidative damage /
- chlorophyll /
- superoxide dismutase /
- malonaldehyde /
- reactive oxygen
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张浩, 罗义, 周启星. 四环素类抗生素生态毒性研究进展[J]. 农业环境科学学报, 2008, 27(2):407-413 Zhang H, Luo Y, Zhou Q X. Research advancement of eco-toxicity of tetracycline antibiotics[J]. Journal of Agro-Environment Science, 2008, 27(2):407-413(in Chinese)
Xu W, Zhang G, Li X, et al. Occurrence and elimination of antibiotics at four sewage treatment plants in the Pearl River Delta (PRD), South China[J]. Water Research, 2007, 41(19):779-786 Xie X J, Zhou Q X, Lin D S, et al. Toxic effect of tetracycline exposure on growth, antioxidative and genetic indices of wheat (Triticum aestivum L.)[J]. Environmental Science and Pollution Research International, 2011, 18(4):566-575 Halling-Rensen B, Nielsen S N, Lanzky P F, et al. Occurrence, fate and effects of pharmaceutical substances in the environment-A review[J]. Chemosphere, 1998, 36(2):357-393 Martinez J L. Environmental pollution by antibiotics and by antibiotic resistance determinants[J]. Environmental Pollution, 2009, 157(11):2893-2902 贺德春, 许振成, 吴根义, 等. 四环素类抗生素的环境行为研究进展[J]. 动物医学进展, 2011, 32(4):98-102 He D C, Xu Z C, Wu G Y, et al. Progress on residues and environmental behavior of tetracycline antibiotics[J]. Progress in Veterinary Medicine, 2011, 32(4):98-102(in Chinese)
张迪, 厉圆, 沈忱思, 等. 金霉素及其异构体降解产物对斜生栅藻的毒性效应研究[J]. 农业环境科学学报, 2019, 38(4):756-764 Zhang D, Li Y, Shen C S, et al. Understanding the toxic effects of chlortetracyclineandits and its isomer degradation products on Scenedesmus obliquus[J]. Journal of Agro-Environment Science, 2019, 38(4):756-764(in Chinese)
K ü mmerer K. Antibiotics in the aquatic environment-A review-Part I[J]. Chemosphere, 2009, 75(4):417-434 Wu P P, Shen H Y. Acute and subacute toxicities effect of oxytetracycline pharmaceutical wastewater on zebrafish[J]. IOP Conference Series:Earth and Environmental Science, 2018, 113:012142 Kolpin D W, Furlong E T, Meyer M T, et al. Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999-2000:A national reconnaissance[J]. Environmental Science and Technology, 2002, 36(6):1202-1211 Monteiro S H, Francisco J G, Andrade G C R M, et al. Study of spatial and temporal distribution of antimicrobial in water and sediments from caging fish farms by on-line SPE-LC-MS/MS[J]. Journal of Environmental Science and Health, Part B, 2016, 51(9):634-643 Yang J F, Ying G G, Zhao J L, et al. Spatial and seasonal distribution of selected antibiotics in surface waters of the Pearl Rivers, China[J]. Journal of Environmental Science and Health, Part B, 2011, 46(3):272-280 Yan C, Yang Y, Zhou J, et al. Antibiotics in the surface water of the Yangtze Estuary:Occurrence, distribution and risk assessment[J]. Environmental Pollution, 2013, 175(8):22-29 Jiang Y, Li M, Guo C, et al. Distribution and ecological risk of antibiotics in a typical effluent-Receiving river (Wangyang River) in the North China[J]. Chemosphere, 2014, 112:267-274 Ferreira C S G, Nunes B A, de Melo Henriques-Almeida J M, et al. Acute toxicity of oxytetracycline and florfenicol to the microalgae Tetraselmis chuii and to the crustacean Artemia parthenogenetica[J]. Ecotoxicology and Environmental Safety, 2007, 67(3):452-458 吴妹英. 鳗鱼养殖常用药物对铜绿微囊藻繁殖的影响[J]. 福建水产, 2009(4):15-21 Wu M Y. The effects of common pesticide in eel culture on the growth of Microcystis aeruginosa[J]. Journal of Fujian Fishery, 2009 (4):15-21(in Chinese)
赵素芬, 曹日波. 土霉素、氯霉素对湛江等鞭金藻细胞增殖和光合色素含量的影响[J]. 广东海洋大学学报, 2004, 24(3):19-23 Zhao S F, Cao R B. Effects of terramycin and ramphenicol on the growth of Isochrysis zhanjianggensis[J]. Journal of Guangdong Ocean University, 2004, 24(3):19-23(in Chinese)
田智宇, 曹卫宇, 邹晓岱. 土霉素废渣对典型植物发芽影响研究[J]. 化肥工业, 2016, 43(6):82-86 Tian Z Y, Cao W Y, Zou X D. Study of effect of oxytetracycline residue on germination of typical plants[J]. Chemical Fertilizer Industry, 2016, 43(6):82-86(in Chinese)
闻洋, 陈寒嫣, 杨炳君, 等. 典型兽用抗生素对斑马鱼的急性毒性及氧化应激的影响[J]. 吉林师范大学学报:自然科学版, 2018, 39(2):94-98 Wen Y, Chen H Y, Yang B J, et al. Acute toxicity and impact on antibiotics of veterinary antibiotics to zebrafish[J]. Journal of Jilin Normal University:Natural Science Edition, 2018, 39(2):94-98(in Chinese)
高问, 武鹏鹏, 王雅学, 等. 土霉素废水对斑马鱼的生物毒性效应研究[J]. 河北科技大学学报, 2019, 40(1):38-44 Gao W, Wu P P, Wang Y X, et al. Study of the biological toxicity of oxytetracycline wastewater on zebrafish[J]. Journal of Hebei University of Science and Technology, 2019, 40(1):38-44(in Chinese)
沈洪艳, 吴志刚, 高吉喜, 等. 青土霉素废水出水对斑马鱼抗氧化指标的影响[J]. 河南科技大学学报:自然科学版, 2013, 34(3):78-82 , 111 Shen H Y, Wu Z G, Gao J X, et al. Effects of wastewater effluent of penicillin and oxytetracycline on antioxidant indexes of Danio rerio[J]. Journal of Henan University of Science and Technology:Natural Science, 2013, 34(3):78-82, 111(in Chinese)
秦洪伟, 陈柳芳, 鲁楠, 等. 氧氟沙星对斜生栅藻的毒性效应[J]. 环境化学, 2011, 30(4):885-886 Qin H W, Chen L F, Lu N, et al. Toxic effects of ofloxacin on Scenedesmus obliquus[J]. Environmental Chemistry, 2011, 30(4):885-886(in Chinese)
李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2000:26-28 Wei M X, Wu Y M, Chen D Z, et al. Changes of free radicals and digestive enzymes in saliva in cases with deficiency in spleen-yin syndrome[J]. Journal of Nanjing Medical University, 2010, 24(3):250-255 Liu L. Oxidative stress induces gastric submucosal arteriolar dysfunction in the elderly[J]. World Journal of Gastroenterology, 2013, 19(48):9439-9446 刘露, 丁柳丽, 陈伟洲, 等. 不同温度下CO2浓度增高对坛紫菜生长和叶绿素荧光特性的影响[J]. 生态学报, 2013, 33(13):3916-3924 Liu L, Ding L L, Chen W Z, et al. The combined effects of increasing CO2 concentrations and different temperatures on the growth and chlorophyll fluorescence in Porphyra haitanensis (Bangiales, Rhodophyta)[J]. Acta Ecologica Sinica, 2013, 33(13):3916-3924(in Chinese)
Wang D, Li S W, Ma T, et al. A comparison study on the effects of norfloxacin on the superoxide dismutase activities in two kinds of sturgeons[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(7):34-37 梁惜梅, 鹿金雁, 聂湘平, 等. 饲料添加剂叔丁基对羟基茴香醚和抗生素诺氟沙星对剑尾鱼的毒性效应[J]. 环境科学学报, 2010, 30(1):172-179 Liang X M, Lu J Y, Nie X P, et al. Toxicity of the food additive BHA (butylated hydroxyanisole) and antibiotic NFLX (norfloxacin) to swordtail fish (Xiphophorus helleri)[J]. Acta Scientiae Circumstantiae, 2010, 30(1):172-179(in Chinese)
Lai T H, He B Y, Zhou R Q, et al. Effects of cadmium stress on the activities of antioxidant enzymes, digestive enzymes and the membrane lipid peroxidation of the mangrove mud clam Geloina coaxans (Gmelin)[J]. Acta Ecologica Sinica, 2011, 31(11):3044-3053 张芬琴, 谢宗平, 王晓琴, 等. 工业废水对黄瓜幼苗生长及叶片抗氧化系统的影响[J]. 干旱地区农业研究, 2006(4):76-81 Zhang F Q, Xie Z P, Wang X Q, et al. Effects of nutriment solution with industrial wastewater on antioxidant system in leaf tissues of cucumber seedlings[J]. Agricultural Research in the Arid Areas, 2006 (4):76-81(in Chinese)
李伟民, 尹大强, 周岩, 等. 五氯酚对鲫鱼肝脏的氧化损伤[J]. 农村生态环境, 2003(1):40-42 Li W M, Yin D Q, Zhou Y, et al. Pentachlorophenol-induced oxidative stress in liver of crucian carp[J]. Rural Eco-Environment, 2003 (1):40-42(in Chinese)
Papadimitriou E, Loumbourdis N S. Exposure of the frog Rana ridibunda to copper:Impact on two biomarkers, lipid peroxidation, and glutathione[J]. Bulletin of Environmental Contamination & Toxicology, 2002, 69(6):0885-0891 沈洪艳, 焦晓会, 武彤. 头孢噻肟钠对斑马鱼SOD活性、MDA含量及DNA损伤的影响[J]. 环境科学学报, 2015, 35(8):2626-2632 Shen H Y, Jiao X H, Wu T. Effects of cefotaxime sodium on SOD activity, MDA content and DNA damage in zebrafish[J]. Acta Scientiae Circumstantiae, 2015, 35(8):2626-2632(in Chinese)
柯跃斌. 自由基毒理学[M]. 北京:人民卫生出版社, 2012:46-48 Valko M, Leibfritz D, Moncol J, et al. Free radicals and antioxidants in normal physiological functions and human disease[J]. The International Journal of Biochemistry & Cell Biology, 2006, 39(1):44-84 余彬彬, 金婷, 张天莹, 等. 铜和土霉素复合暴露对玉米幼苗生长发育的影响[J]. 环境与健康杂志, 2016, 33(12):1082-1085 Yu B B, Jin T, Zhang T Y, et al. Effects of copper and oxytetracycline combined exposure on the seedling development of maize[J]. Journal of Environment and Health, 2016, 33(12):1082-1085(in Chinese)
刘永涛, 李乐, 杨红, 等. 3种渔用药物对斜生栅藻的毒性效应研究[J]. 生态环境学报, 2017, 26(2):261-267 Liu Y T, Li L, Yang H, et al. Toxic effects of three fishery drugs on Scenedesmus obliquus[J]. Ecology and Environmental Sciences, 2017, 26(2):261-267(in Chinese)
You R R, Sun H Y, Yu Y, et al. Time-dependent hormesis of chemical mixtures:A case study on sulfa antibiotics and a quorum-sensing inhibitor of Vibrio fischeri[J]. Environmental Toxicology and Pharmacology, 2016, 41:45-53 吴晓娟, 陈峥宏, 王菲. 抗生素对幽门螺杆菌细胞壁缺陷的影响[J]. 江苏医药, 2013, 39(2):132-134 Wu X J, Chen Z H, Wang F. Effect of antibiotics on cell wall deficiency of Helicobacter pylori[J]. Jiangsu Medical Journal, 2013, 39(2):132-134(in Chinese)
张志林, 顾亚明, 吴菊芳, 等. 影响细胞壁合成的抗生素引起金黄色葡萄球菌超微结构的改变[J]. 上海第一医学院学报, 1983(5):339-342,338 -402 Zhang Z L, Gu Y M, Wu J F, et al. Ultrastructural changes of Staphylococcus aureus caused by cell wall synthesis inhibiting antibiotics[J]. Acta Academiae Medicinae Primae Shanghai, 1983(5):339-342, 338-402(in Chinese)
周旭东. 四环素类、磺胺类抗生素对铜绿微囊藻和小球衣藻的影响研究[D]. 杨凌:西北农林科技大学, 2017:14-15 Zhou X D. Effects of tetracyclines and sulfonamides on Microcystis aeruginosa and Chlamydomonas microsphaera[D]. Yangling:Northwest A & F University, 2017:14 -15(in Chinese)
姚建华, 牛德奎, 李兆君, 等. 抗生素土霉素对小麦根际土壤酶活性和微生物生物量的影响[J]. 中国农业科学, 2010, 43(4):721-728 Yao J H, Niu D K, Li Z J, et al. Effects of antibiotics oxytetracycline on soil enzyme activities and microbial biomass in wheat rhizosphere[J]. Scientia Agricultura Sinica, 2010, 43(4):721-728(in Chinese)
杜胜蓝, 黄岁樑, 臧常娟, 等. 浮游植物现存量表征指标间相关性研究Ⅱ:叶绿素a与藻密度[J]. 水资源与水工程学报, 2011, 22(2):44-49 Du S L, Huang S L, Zang C J, et al. Correlation research between the indicators of phytoplankton standing stockⅡ:Chlorophyll a and algal density[J]. Journal of Water Resources and Water Engineering, 2011, 22(2):44-49(in Chinese)
鲍洁, 苏振霞, 肖辉. 盐酸恩诺沙星对斜生栅藻(Scenedesmus obliquus)的致毒效应[J]. 淮海工学院学报:自然科学版, 2015, 24(3):84-87 Bao J, Su Z X, Xiao H. Toxic effects of enrofloxacin hydrochloride on Scenedesmus obliquus[J]. Journal of Huaihai Institute of Technology:Natural Science Edition, 2015, 24(3):84-87(in Chinese)
Liu T, Zhu L, Xie H, et al. Effects of the ionic liquid 1-octyl-3-methylimidazolium hexafluorophosphate on the growth of wheat seedlings[J]. Environmental Science & Pollution Research, 2014, 21(5):3936-3945 李娟, 王应军, 高鹏. 过氧化氢对铜绿微囊藻的损伤效应研究[J]. 环境科学学报, 2015, 35(4):1183-1189 Li J, Wang Y J, Gao P. Damaging effects of hydrogen peroxide on Microcystis aeruginosa[J]. Acta Scientiae Circumstantiae, 2015, 35(4):1183-1189(in Chinese)
Kuang X, Gu J D, Tie B Q, et al. Interactive effects of cadmium and Microcystis aeruginosa (cyanobacterium) on the growth, antioxidative responses and accumulation of cadmium and microcystins in rice seedlings[J]. Ecotoxicology, 2016, 25(8):1588-1599 沈洪艳, 曹志会, 赵月, 等. 抗生素药物诺氟沙星对锦鲤的毒性效应[J]. 安全与环境学报, 2015, 15(4):380-385 Shen H Y, Cao Z H, Zhao Y, et al. Study on toxicity of antibiotic norfloxacin to Cyprinus carpio[J]. Journal of Safety and Environment, 2015, 15(4):380-385(in Chinese)
沈洪艳, 吴志刚, 高吉喜, 等. 抗生素药物环丙沙星对锦鲤抗氧化系统的毒性作用[J]. 安全与环境学报, 2014, 14(3):328-333 Shen H Y, Wu Z G, Gao J X, et al. Toxicity effect of ciprofloxacin hydrochloride exposure to the antioxidant systems on Cyprinus carpio[J]. Journal of Safety and Environment, 2014, 14(3):328-333(in Chinese)
喻燚, 李巧玉, 董聪聪, 等. 4-壬基酚对拟柱胞藻生长、抗氧化酶和光合作用的影响及机理[J]. 生态毒理学报, 2018, 13(6):259-267 Yu Y, Li Q Y, Dong C C, et al. Effect and mechanism of 4-nonylphenol on the growth, antioxidative enzymes and photosynthesis of Cylindrospermopsis raciborskii[J]. Asian Journal of Ecotoxicology, 2018, 13(6):259-267(in Chinese)
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