摘要:
以高粱(熊杂9号)为试材,研究酸雨胁迫下镧对高粱种子萌发、过氧化物酶(POD)活性、可溶性蛋白、丙二醛(MDA)含量和质膜透性的影响.结果显示,用12 mg·l-1的LaCl溶液浸种处理高粱种子效果最佳.经La(Ⅲ)处理后,种子发芽率、发芽势、发芽指数和活力指数均较单一酸雨处理组升高,pH 2.5时增幅最明显;pH 2.0时,酸雨严重破坏种子内部结构,La(Ⅲ)的加入已无法缓解酸雨引发的伤害.而经La(Ⅲ)处理后,低强度(pH 5.0-3.5)酸雨胁迫下,种子的POD活性较单一酸雨处理组升高,高强度(pH 3.0-2.0)时降低;可溶性蛋白含量较所有单一酸雨处理组升高,MDA含量和质膜透性较单一酸雨处理降低,pH 2.5-2.0时该效果尤为明显.表明La(Ⅲ)可通过调节高粱种子内部的POD活性减轻酸雨胁迫引发的膜脂过氧化,缓解对种子萌发的伤害.
关键词:
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镧
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酸雨
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胁迫
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高粱
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萌发
Abstract:
The effects of lanthanum on seed germination,malondialdehyde(MDA)content,plasolemma permeability,soluble protein content and peroxidase(POD)activity of sorghum under acid rain stress were investigated after soaked in LaCl3 solution for 12h.Seeds were germinated in simulated acid rain solution in culture container.The purpose was to provide method and technical guide for alleviating and resisting the damage of acid rain to seed germination.A significant effects of La(Ⅲ)compare with check groups(CK)was evident: sorghum seeds were germinated best after soaked with 12 mg·l-1LaCl3 solution.After seeds treated with La(Ⅲ),the damage of acid rain to seed germination decreased.Percentage of germination,germination energy,germination index,vigor index were higher than those of treatment groups without La(Ⅲ),especially for the treatment of pH 2.5.La(Ⅲ)couldn't alleviate the damage of acid rain under pH 2.0 for the internal structure of seeds were destroyed.POD activity were higher than those of treatment groups without La(Ⅲ)under acid rain stress(pH 5.0-3.5),while lower when the pH from 3.0 to 2.0.The soluble protein content was higher than those of treatment groups without La(Ⅲ).And the MDA content and plasolemma permeability of La(Ⅲ)treatment groups were lower than those of treatment groups without La(Ⅲ),especially for the pH 2.5 and pH 2.0.It showed La(Ⅲ)could alleviate the damage of acid rain to seed germination,by adjusting the POD activity to decrease the membrane lipid peroxidation induced by acid rain.
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