摘要:
以小鼠为对象,对硝基苯(NB)的蓄积毒性和氧化损伤进行研究,检测SOD,GSH-Px,CAT,MDA和蛋白质羰基含量.结果表明,蓄积系数(K)为4.76;亚急性试验随着染毒剂量的加大,肾脏和脾脏的MDA含量逐渐升高,CAT,SOD和GSH-Px酶活性逐渐降低,蛋白质羰基含量呈上升趋势,并表现出明显的剂量效应.表明NB对肾脏和脾脏具有明显的毒性作用,NB可使自由基在机体内累积,引起蛋白质氧化损伤,导致机体发生氧化应激.
关键词:
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硝基苯
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蓄积毒性
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氧化损伤
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小鼠
Abstract:
In the study of accumulative action,the periodically increasing dosimetry was adopted.100 male Kunming mice were divided into 5 groups.Group Ⅰ was the control group.Group Ⅱ was the oil control group.The dosage of NB in group Ⅲ,Ⅳ and V were 26 mg·kg-1BW,52 mg·kg-1BW and 105 mg·kg-1BW respectively(once a day).The experimental period was 20 d.The activity of SOD,GSH-Px and CAT and the content of MDA and protein carbonyl were detected.The results showed that the accumulation coefficient K was 4.76.With the increasing dosage of nitrobenzene(NB),the content of MDA and protein carbonyl gradually increased,and the activity of CAT,SOD and GSH-Px gradually decreased in kidney and spleen.The results indicate that NB could produce significantly toxic effect on the kidney and spleen through the accumulation of the free radical,the protein oxidative damage and the oxidative stress in the organism.
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