铜绿假单胞菌(TBPY)降解对氯苯酚的特性

李晓菊; 姚日生; 王淮; 程莎莎

环境化学

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李晓菊, 姚日生, 王淮, 程莎莎. 铜绿假单胞菌(TBPY)降解对氯苯酚的特性[J]. 环境化学, 2010, 29(5): 914-918.

引用本文:

李晓菊, 姚日生, 王淮, 程莎莎. 铜绿假单胞菌(TBPY)降解对氯苯酚的特性[J]. 环境化学, 2010, 29(5): 914-918.

LI Xiaoju, YAO Risheng, WANG Huai, CHENG Shasha. THE STUDY ON BIODEGRADATION OF 4-CHLOROPHEMOL BY PSEUDOMONAS AERUGINOSA TBPY[J]. Environmental Chemistry, 2010, 29(5): 914-918.

Citation:

LI Xiaoju, YAO Risheng, WANG Huai, CHENG Shasha. THE STUDY ON BIODEGRADATION OF 4-CHLOROPHEMOL BY PSEUDOMONAS AERUGINOSA TBPY[J]. Environmental Chemistry, 2010, 29(5): 914-918.

铜绿假单胞菌(TBPY)降解对氯苯酚的特性

1.
合肥工业大学化工学院, 合肥, 230009;
2.
合肥工业大学农产品生物化工教育部工程研究中心, 合肥, 230009
基金项目:
安徽省教育厅自然科学基金(KJ2009A086)资助

THE STUDY ON BIODEGRADATION OF 4-CHLOROPHEMOL BY PSEUDOMONAS AERUGINOSA TBPY

1.
School of Chemistry and Engineering, Hefei University of Technology, Hefei, 230009, China;
2.
Engineering Reasearch Center of Bio-Process, Ministry of Education, PRC, Hefei University of Technology, Hefei, 230009, China
Fund Project:

摘要: 借助Vis-UV分光光度计、高效液相色谱(HPLC)仪和透射电镜(TEM),对铜绿假单胞菌TBPY的生长与降解对氯苯酚的性能进行了研究.结果表明:通过逐步增加对氯苯酚浓度和转接代数的驯化后,TBPY菌株降解对氯苯酚的能力有了很大的提高,原菌在4d内才能将浓度为100mg·l-1的对氯苯酚降解至85mg·l-1,降解率只有15%,而多次驯化后的TBPY只需2d就能将浓度为100mg·l-1的对氯苯酚完全降解,驯化能显著提高TBPY菌株的降解能力;并且,与驯化前相比菌体的形态发生了很大变化,由菌体周边整齐、直或稍弯、两端钝圆的均匀杆状变为不均匀的卵圆、短杆和直杆状,几乎每个菌的形态都呈"花生"状,且菌体周边出现半透明的膜状物质,TBPY有很好的自我保护能力.随着对氯苯酚浓度的增大,TBPY菌的生长迟滞期是逐渐延长的,当对氯苯酚浓度达到150mg·l-1时,生长迟滞期为6d,对氯苯酚浓度继续增加,TBPY几乎停止生长,TBPY可耐受150mg·l-1的对氯苯酚.温度在30℃左右时,对氯苯酚的初始降解速率最大;菌株TBPY在培养基初始pH值为7.0—8.0的范围内,降解对氯苯酚的能力较强,以pH7.5的降解效果最好,对氯苯酚的初始降解速率高达1.51mg·l-1·h-1;当pH6.5和pH8.5时,对氯苯酚的初始降解速率均较低为0.513mg·l-1·h-1和1.061mg·l-1·h-1.该菌优化降解条件为:温度30℃、培养基初始pH7.5、接种量2.0%;在该条件下,发酵1d后100mg·l-1对氯苯酚的降解率达99%以上.
- 生物降解 /
- 对氯苯酚 /
- 铜绿假单胞菌TBPY
Abstract: An aerobic bacterial strain Pseudomonas aeruginosa TBPY was identified to have 4-chlorophenol-degrading capability after acclimation. The growth of Pseudomonas aeruginosa TBPY and the degradation performance of 4-chlorophenol were studied with UV-Vis spectrophotometey, high performance liquid chromatography (HPLC) and transmission electron microscopy (TEM). Results showed that the 4-chlorophenol-degrading capability of TBPY increased greatly through acclimation. The degradation ratio of 4-chlorophenol by TBPY before acclimation was only 15%in 4 days when the initial concentration was 100mg·l-1. However, TBPY after acclimation degraded 4-chlorophenol completely in 2 days when the initial concentration was 100mg·l-1. In addition, the shape of TBPY before and after acclimation changed greatly. TBPY before acclimation was neat, straight or slightly curved, blunt at both ends with a uniform circular rod-shape. TBPY after acclimation was uneven, short rod and straight rod-shaped. Most of the bacterial after acclimation were peanut shell-like. Bacterial periphery was translucent.They had a good self-protection ability. Lag period was extended with the increase of 4-chlorophenol concentration. Lag period of TBPY was 6 days when 4-chlorophenol concentration was 150mg·l-1.If the concentration of 4-chlorophenol continued to increase, TBPY virtually stopped growing. The highest endurance capability of TBPY was about 150mg·l-1 4-chlorophenol. The initial degradation rate of 4-chlorophenol was maximum at 30℃. The 4-chlorophenol initial degradation rate was lower at pH 6.5 and pH 8.5(only 0.513 mg·l-1·h-1 and 1.061 mg·l-1·h-1, respectively). However, the initial degradation rate was higher when pH was between 7.0 and 8.0, and it was maximum(up to 1.51mg·l-1·h-1) at pH 7.5.The degradation ratio was over 99% in 1d when 4-chlorophenol concentration was 100mg·l-1, the temperature was 30℃, the initial medium pHvalue was 7.5, and the inoculation volume was 2.0%.
- biodegradation /
- 4-chlorophenol /
- Pseudomonas aeruginosa TBPY

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[12]	陶建伟,高永红,陆庆宁.反相高效液相色谱法同时测定对苯氧基苯酚和对氯苯酚[J].分析试验室,2006,25(2):13-15
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李晓菊, 姚日生, 王淮, 程莎莎. 铜绿假单胞菌(TBPY)降解对氯苯酚的特性[J]. 环境化学, 2010, 29(5): 914-918.

引用本文:

李晓菊, 姚日生, 王淮, 程莎莎. 铜绿假单胞菌(TBPY)降解对氯苯酚的特性[J]. 环境化学, 2010, 29(5): 914-918.

LI Xiaoju, YAO Risheng, WANG Huai, CHENG Shasha. THE STUDY ON BIODEGRADATION OF 4-CHLOROPHEMOL BY PSEUDOMONAS AERUGINOSA TBPY[J]. Environmental Chemistry, 2010, 29(5): 914-918.

Citation:

LI Xiaoju, YAO Risheng, WANG Huai, CHENG Shasha. THE STUDY ON BIODEGRADATION OF 4-CHLOROPHEMOL BY PSEUDOMONAS AERUGINOSA TBPY[J]. Environmental Chemistry, 2010, 29(5): 914-918.

铜绿假单胞菌(TBPY)降解对氯苯酚的特性

1. 合肥工业大学化工学院, 合肥, 230009;
2. 合肥工业大学农产品生物化工教育部工程研究中心, 合肥, 230009

收稿日期: 2009-10-18

基金项目:

安徽省教育厅自然科学基金(KJ2009A086)资助

关键词:

摘要: 借助Vis-UV分光光度计、高效液相色谱(HPLC)仪和透射电镜(TEM),对铜绿假单胞菌TBPY的生长与降解对氯苯酚的性能进行了研究.结果表明:通过逐步增加对氯苯酚浓度和转接代数的驯化后,TBPY菌株降解对氯苯酚的能力有了很大的提高,原菌在4d内才能将浓度为100mg·l-1的对氯苯酚降解至85mg·l-1,降解率只有15%,而多次驯化后的TBPY只需2d就能将浓度为100mg·l-1的对氯苯酚完全降解,驯化能显著提高TBPY菌株的降解能力;并且,与驯化前相比菌体的形态发生了很大变化,由菌体周边整齐、直或稍弯、两端钝圆的均匀杆状变为不均匀的卵圆、短杆和直杆状,几乎每个菌的形态都呈"花生"状,且菌体周边出现半透明的膜状物质,TBPY有很好的自我保护能力.随着对氯苯酚浓度的增大,TBPY菌的生长迟滞期是逐渐延长的,当对氯苯酚浓度达到150mg·l-1时,生长迟滞期为6d,对氯苯酚浓度继续增加,TBPY几乎停止生长,TBPY可耐受150mg·l-1的对氯苯酚.温度在30℃左右时,对氯苯酚的初始降解速率最大;菌株TBPY在培养基初始pH值为7.0—8.0的范围内,降解对氯苯酚的能力较强,以pH7.5的降解效果最好,对氯苯酚的初始降解速率高达1.51mg·l-1·h-1;当pH6.5和pH8.5时,对氯苯酚的初始降解速率均较低为0.513mg·l-1·h-1和1.061mg·l-1·h-1.该菌优化降解条件为:温度30℃、培养基初始pH7.5、接种量2.0%;在该条件下,发酵1d后100mg·l-1对氯苯酚的降解率达99%以上.

English Abstract

THE STUDY ON BIODEGRADATION OF 4-CHLOROPHEMOL BY PSEUDOMONAS AERUGINOSA TBPY

1. School of Chemistry and Engineering, Hefei University of Technology, Hefei, 230009, China;
2. Engineering Reasearch Center of Bio-Process, Ministry of Education, PRC, Hefei University of Technology, Hefei, 230009, China

Received Date: 2009-10-18

Fund Project:

Keywords:

Abstract: An aerobic bacterial strain Pseudomonas aeruginosa TBPY was identified to have 4-chlorophenol-degrading capability after acclimation. The growth of Pseudomonas aeruginosa TBPY and the degradation performance of 4-chlorophenol were studied with UV-Vis spectrophotometey, high performance liquid chromatography (HPLC) and transmission electron microscopy (TEM). Results showed that the 4-chlorophenol-degrading capability of TBPY increased greatly through acclimation. The degradation ratio of 4-chlorophenol by TBPY before acclimation was only 15%in 4 days when the initial concentration was 100mg·l-1. However, TBPY after acclimation degraded 4-chlorophenol completely in 2 days when the initial concentration was 100mg·l-1. In addition, the shape of TBPY before and after acclimation changed greatly. TBPY before acclimation was neat, straight or slightly curved, blunt at both ends with a uniform circular rod-shape. TBPY after acclimation was uneven, short rod and straight rod-shaped. Most of the bacterial after acclimation were peanut shell-like. Bacterial periphery was translucent.They had a good self-protection ability. Lag period was extended with the increase of 4-chlorophenol concentration. Lag period of TBPY was 6 days when 4-chlorophenol concentration was 150mg·l-1.If the concentration of 4-chlorophenol continued to increase, TBPY virtually stopped growing. The highest endurance capability of TBPY was about 150mg·l-1 4-chlorophenol. The initial degradation rate of 4-chlorophenol was maximum at 30℃. The 4-chlorophenol initial degradation rate was lower at pH 6.5 and pH 8.5(only 0.513 mg·l-1·h-1 and 1.061 mg·l-1·h-1, respectively). However, the initial degradation rate was higher when pH was between 7.0 and 8.0, and it was maximum(up to 1.51mg·l-1·h-1) at pH 7.5.The degradation ratio was over 99% in 1d when 4-chlorophenol concentration was 100mg·l-1, the temperature was 30℃, the initial medium pHvalue was 7.5, and the inoculation volume was 2.0%.

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铜绿假单胞菌(TBPY)降解对氯苯酚的特性

THE STUDY ON BIODEGRADATION OF 4-CHLOROPHEMOL BY PSEUDOMONAS AERUGINOSA TBPY

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铜绿假单胞菌(TBPY)降解对氯苯酚的特性

English Abstract

THE STUDY ON BIODEGRADATION OF 4-CHLOROPHEMOL BY PSEUDOMONAS AERUGINOSA TBPY

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