复合固定化法固定微生物去除芘

李婧; 党志; 郭楚玲; 卢桂宁; 卢静

环境化学

复合固定化法固定微生物去除芘

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李婧, 党志, 郭楚玲, 卢桂宁, 卢静. 复合固定化法固定微生物去除芘[J]. 环境化学, 2012, 31(7): 1036-1042.

引用本文:

李婧, 党志, 郭楚玲, 卢桂宁, 卢静. 复合固定化法固定微生物去除芘[J]. 环境化学, 2012, 31(7): 1036-1042.

LI Jing, DANG Zhi, GUO Chuling, LU Guining, LU Jing. Removal of pyrene using immobilized microorganism[J]. Environmental Chemistry, 2012, 31(7): 1036-1042.

Citation:

LI Jing, DANG Zhi, GUO Chuling, LU Guining, LU Jing. Removal of pyrene using immobilized microorganism[J]. Environmental Chemistry, 2012, 31(7): 1036-1042.

复合固定化法固定微生物去除芘

1.
华南理工大学环境科学与工程学院, 广州, 510006;
2.
工业聚集区污染控制与生态修复教育部重点实验室, 广州, 510006;
3.
纸浆造纸工程国家重点实验室, 广州, 510640
基金项目:
广东省自然科学基金项目(9351064101000001)

广州市环保局科技成果应用示范项目

广州市珠江新星项目资助.

Removal of pyrene using immobilized microorganism

1.
School of Environmental Science and Engineering, South China University of Technology ,Guangzhou, 510006,China;
2.
The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education,Guangzhou, 510006, China;
3.
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, 510640, China
Fund Project:

摘要: 通过"玉米秸秆吸附-包埋-交联"复合固定化方法固定多环芳烃降解菌GY2B和GP3B,提高对芘的降解性能,并对其作用过程进行初步研究.结果表明,游离态单细菌GY2B、GP3B和混合菌GY2B+GP3B的7 d降解率分别为14.0%、55.0%和73.6%,而固定化GY2B+GP3B秸秆小球在5 d内即达98.2%,去除率得到了显著的提高;对固定化微生物小球的比表面积、孔隙率和扫描电镜图分析,说明载体表面具有较高的比表面积,内部是具有大量孔隙的骨架结构,在内部生长的微生物和基质有充分的接触面积和机会;固定化GY2B+GP3B秸秆小球类似于一个吸附降解一体化的微型反应器.GY2B+GP3B混合菌芘代谢产物有菲-4-羧酸、二甲基酞酸、1-羟基-2-萘酸、1-萘酚和水杨酸,推测芘的降解是GY2B+GP3B混合菌集体代谢的综合作用的结果,GP3B的中间转化产物作为GY2B生长代谢的基质,使得芘得到更完全的降解.
- 芘 /
- 固定化微生物 /
- 生物降解 /
- 玉米秸秆 /
- 代谢产物
Abstract: In order to improve the degradation of pyrene, immobilized PAHs-degrading bacteria GY2B and GP3B were prepared that involved pre-adsorption on corn stalk (CS), entrapment and cross-linking. The degradation rates and process were studied. Free GY2B, free GP3B and free mixed GY2B+GP3B were incubated individually to test their own pyrene degradation rates in 7 d, which were 14.0%, 55.0% and 73.6%, respectively. On the other hand, the pyrene removal rate of immobilized CS GY2B + GP3B reached 98.2% in 5 d. The analysis of specific surface area, porosity and scanning electron microscopy showed that the interior of the support had a large number of pores and full of skeleton structure. The microorganisms inside had sufficient contact with the substrate. With the addition of corn stalks, the adsorption capacity was enhanced and the removal of pyrene was improved to certain extent. From the GC-MS results of GY2B+GP3B metabolites, phenanthrene-4-carboxylate, dimethylated phthalic acid, 1-hydroxy-2-naphthoic acid, 1-naphthol and salicylic acid were identified. It is likely that degradation of pyrene was the cooperative result by mixed GY2B + GP3B bacteria. And the intermediate metabolites of GP3B were used as the substrates by GY2B, which made the pyrene degradation more complete.
- pyrene /
- immobilization /
- biodegradation /
- corn stalk /
- metabolism

[1]	Peng R, Xiong A, Xue Y, et al. Microbial biodegradation of polyaromatic hydrocarbons[J]. FEMS Microbiology Reviews, 2008, 32(6): 927-955
[2]	Martorell I, Perelló G, Martí-Cid R, et al. Polycyclic aromatic hydrocarbons (PAH) in foods and estimated PAH intake by the population of Catalonia, Spain: Temporal trend[J]. Journal of Food Protection, 2010, 36(5): 424-432
[3]	Somtrakoon K, Suanjit S, Pokethitiyook P, et al. Phenanthrene stimulates the degradation of pyrene and fluoranthene by Burkholderia sp. VUN10013[J]. World Journal of Microbiology and Biotechnology. 2008, 24(4): 523-531
[4]	曲洋,张培玉,郭沙沙,等. 复合固定化法固定化微生物技术在污水生物处理中的研究应用[J]. 四川环境, 2009, 28(3): 78-84
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[6]	陈晓鹏,易筱筠,陶雪琴,等. 石油污染土壤中芘高效降解菌群的筛选及降解特性研究[J]. 环境工程学报, 2008, 2(3): 413-417
[7]	Tao X, Lu G, Dang Z, et al. A phenanthrene-degrading strain Sphingomonas sp. GY2B isolated from contaminated soils[J]. Process Biochemistry, 2007, 42(3): 401-408
[8]	Wang Y, Yang X, Tu W, et al. High-rate ferrous iron oxidation by immobilized Acidithiobacillus ferrooxidans with complex of PVA and sodium alginate[J]. Journal of Microbiological Methods, 2007, 68(2): 212-217
[9]	白雪,周林成,李彦锋,等. 聚乙烯醇载体制备及其固定化微生物处理污水研究进展[J]. 离子交换与吸附, 2010, 26(4): 377-384
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[11]	Yang S, Jin H, Wei Z, et al. Bioremediation of oil spills in cold environments: A review[J]. Pedosphere, 2009, 19(3): 371-381
[12]	Cerniglia C E. Biodegradation of polycyclic aromatic hydrocarbons[J]. Biodegradation, 1992, 3(2): 351-368
[13]	Romero M C, Salvioli M L, Cazau M C, et al. Pyrene degradation by yeasts and filamentous fungi[J]. Environmental Pollution, 2002, 117(1): 159-163
[14]	Li X, Cullen W R, Reimer K J, et al. Microbial degradation of pyrene and characterization of a metabolite[J]. Science of the Total Environment, 1996, 177(1-3): 17-29
[15]	Prabhu Y, Phale P S. Biodegradation of phenanthrene by Pseudomonas sp. strain PP2: novel metabolic pathway, role of biosurfactant and cell surface hydrophobicity in hydrocarbon assimilation [J]. Applied Microbiology and Biotechnology, 2003, 61(4): 342-351
[16]	Dean-Ross D, Cerniglia C E. Degradation of pyrene by Mycobacterium flavescens [J]. Appl Microbiol Biotechnol. 1996, 46: 307-312
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[18]	Kim S, Kweon O, Jones R C, et al. Complete and integrated pyrene degradation pathway in Mycobacterium vanbaalenii PYR-1 based on systems biology[J]. J Bacteriol, 2007, 189(2): 464-472

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李婧, 党志, 郭楚玲, 卢桂宁, 卢静. 复合固定化法固定微生物去除芘[J]. 环境化学, 2012, 31(7): 1036-1042.

引用本文:

李婧, 党志, 郭楚玲, 卢桂宁, 卢静. 复合固定化法固定微生物去除芘[J]. 环境化学, 2012, 31(7): 1036-1042.

LI Jing, DANG Zhi, GUO Chuling, LU Guining, LU Jing. Removal of pyrene using immobilized microorganism[J]. Environmental Chemistry, 2012, 31(7): 1036-1042.

Citation:

LI Jing, DANG Zhi, GUO Chuling, LU Guining, LU Jing. Removal of pyrene using immobilized microorganism[J]. Environmental Chemistry, 2012, 31(7): 1036-1042.

复合固定化法固定微生物去除芘

1. 华南理工大学环境科学与工程学院, 广州, 510006;
2. 工业聚集区污染控制与生态修复教育部重点实验室, 广州, 510006;
3. 纸浆造纸工程国家重点实验室, 广州, 510640

收稿日期: 2012-02-25

基金项目:

广东省自然科学基金项目(9351064101000001)

广州市环保局科技成果应用示范项目

广州市珠江新星项目资助.

关键词:

摘要: 通过"玉米秸秆吸附-包埋-交联"复合固定化方法固定多环芳烃降解菌GY2B和GP3B,提高对芘的降解性能,并对其作用过程进行初步研究.结果表明,游离态单细菌GY2B、GP3B和混合菌GY2B+GP3B的7 d降解率分别为14.0%、55.0%和73.6%,而固定化GY2B+GP3B秸秆小球在5 d内即达98.2%,去除率得到了显著的提高;对固定化微生物小球的比表面积、孔隙率和扫描电镜图分析,说明载体表面具有较高的比表面积,内部是具有大量孔隙的骨架结构,在内部生长的微生物和基质有充分的接触面积和机会;固定化GY2B+GP3B秸秆小球类似于一个吸附降解一体化的微型反应器.GY2B+GP3B混合菌芘代谢产物有菲-4-羧酸、二甲基酞酸、1-羟基-2-萘酸、1-萘酚和水杨酸,推测芘的降解是GY2B+GP3B混合菌集体代谢的综合作用的结果,GP3B的中间转化产物作为GY2B生长代谢的基质,使得芘得到更完全的降解.

English Abstract

Removal of pyrene using immobilized microorganism

1. School of Environmental Science and Engineering, South China University of Technology ,Guangzhou, 510006,China;
2. The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education,Guangzhou, 510006, China;
3. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, 510640, China

Received Date: 2012-02-25

Fund Project:

Keywords:

Abstract: In order to improve the degradation of pyrene, immobilized PAHs-degrading bacteria GY2B and GP3B were prepared that involved pre-adsorption on corn stalk (CS), entrapment and cross-linking. The degradation rates and process were studied. Free GY2B, free GP3B and free mixed GY2B+GP3B were incubated individually to test their own pyrene degradation rates in 7 d, which were 14.0%, 55.0% and 73.6%, respectively. On the other hand, the pyrene removal rate of immobilized CS GY2B + GP3B reached 98.2% in 5 d. The analysis of specific surface area, porosity and scanning electron microscopy showed that the interior of the support had a large number of pores and full of skeleton structure. The microorganisms inside had sufficient contact with the substrate. With the addition of corn stalks, the adsorption capacity was enhanced and the removal of pyrene was improved to certain extent. From the GC-MS results of GY2B+GP3B metabolites, phenanthrene-4-carboxylate, dimethylated phthalic acid, 1-hydroxy-2-naphthoic acid, 1-naphthol and salicylic acid were identified. It is likely that degradation of pyrene was the cooperative result by mixed GY2B + GP3B bacteria. And the intermediate metabolites of GP3B were used as the substrates by GY2B, which made the pyrene degradation more complete.

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复合固定化法固定微生物去除芘

Removal of pyrene using immobilized microorganism

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复合固定化法固定微生物去除芘

English Abstract

Removal of pyrene using immobilized microorganism

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