不同硫源对红串红球菌生长和脱硫比活性的效应
EFFECTS OF DIFFERENT SULFUR SOURCES ON THE GROWTH AND SPECIFIC DESULFURIZATION ACTIVITY OF RHODOCOCCUS ERYTHROPOLIS
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摘要: 本文以牛磺酸、硫酸镁和二苯噻吩(DBT)分别作为唯一硫源以及DBT不同供给量对红串红球菌生长和脱硫比活性的效应进行了研究.结果表明,红串红球菌具备利用有机和无机硫化合物作为硫源的能力.与牛磺酸和硫酸镁相比,DBT是促进红串红球菌生长和提高其脱硫比活性的最好硫源.在DBT初始浓度为11.5mg·l-1时红串红球菌的最大脱硫比活性达79.1mmol2HBP·kg-1·h-1.影响红串红球菌脱硫比活性的因素主要是其生长的不同阶段、DBT供给量和羟基二苯生产量.用DBT作为硫源,且控制其供给量只满足红串红球菌30%的正常需硫量,是提高其脱硫比活性的优化控制方法.Abstract: The effects of different sulfur sources of taurine, magnesium sulfate and dibenzothiophene (DBT) respectively as a sole sulfur source, and adding amount of DBT on both the growth and specific desulfurization activity of Rhodococcus erythropolis KA2-5-1 were studied in this paper. It was found that R. erytkropolis KA2-5-1 has an ability in using both organic and inorganic sulfur compounds as a sulfur source. Compared with taurine and magnesium sulfate, DBT is found being the best sulfur source for both strain growth and its specific desulfurization activity. The maximum specific desulfurization activity of 79.1mmol 2HBP·kg-1·h-1 was obtained when initial DBT concentration was at 11.5 mg·l-1. The factors affecting specific desulfurizatin activity are suggested as diferent growth phase, adding amount of DBT and the concentratin of 2 hydroxybiphenyl production. Using DBT as a sole sulfur source and limiting its adding amount to the extent of only meeting 30% normal sulfur requirement of R. erytkropolis KA2-5-1 is a optimal control method for the highest specific desulfurization activity.
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