五氯硝基苯对小鼠脑AChE的影响及多器官氧化损伤研究

宋莉莉; 金明华; 刘晓梅; 袁星; 邓铁柱

环境化学

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宋莉莉, 金明华, 刘晓梅, 袁星, 邓铁柱. 五氯硝基苯对小鼠脑AChE的影响及多器官氧化损伤研究[J]. 环境化学, 2006, 25(4): 462-465.

引用本文:

宋莉莉, 金明华, 刘晓梅, 袁星, 邓铁柱. 五氯硝基苯对小鼠脑AChE的影响及多器官氧化损伤研究[J]. 环境化学, 2006, 25(4): 462-465.

SONG Li-li, JIN Ming-hua, LIU Xiao-mei, YUAN Xing, DENG Tie-zhu . STUDY ON THE ACTIVITY OF ACETYLCHOLINESTERASE AND OXIDATION DAMAGE ON DIFFERENT ORGANS OF MICE INDUCED BY PENTACHLORONITROBENZENE[J]. Environmental Chemistry, 2006, 25(4): 462-465.

Citation:

五氯硝基苯对小鼠脑AChE的影响及多器官氧化损伤研究

1.
东北师范大学城市与环境科学学院, 长春, 130024;
2.
吉林大学公共卫生学院, 长春, 130021
基金项目:
教育部科学技术研究重点项目(03058)

STUDY ON THE ACTIVITY OF ACETYLCHOLINESTERASE AND OXIDATION DAMAGE ON DIFFERENT ORGANS OF MICE INDUCED BY PENTACHLORONITROBENZENE

1.
College of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China;
2.
Public Hygiene College, Jilin University, Changchun, 130021, China
Fund Project:

摘要: 测定五氯硝基苯(PCNB)对小鼠脑乙酰胆碱酯酶(AChE)的影响及多器官的氧化损伤作用.对昆明种小鼠采用灌胃法进行200,400和600 mg·kg-1三个剂量染毒,每日一次,分别于染毒4,8,12和16d测定AChE活性、过氧化氢酶(CAT)活性和脂质过氧化(LPO)产物丙二醛(MDA)的含量.结果表明:(1)小鼠脑AChE活性受到抑制,并表现为明显的时间和剂量依赖性;(2)肝、肾CAT活性变化显著,脂质过氧化作用明显;(3)高剂量毒物在染毒16d时对脾脏脂质过氧化作用明显增强;(4)脑和血清CAT活性随染毒时间延长先升高后降低,脂质过氧化作用不明显.PCNB对小鼠可能具有神经毒性,对肝、肾脏的氧化损伤作用较强.
- 五氯硝基苯 /
- 乙酰胆碱酯酶 /
- 氧化损伤 /
- 过氧化氢酶 /
- 脂质过氧化 /
- 小鼠
Abstract: The effects of pentachloronitrobenzene(PCNB) on the activity of acetylcholinesterase(AChE) in brain and oxidation damage in various organs(brain,liver,kidney,spleen and serum) of mice were studied.The mice were poisoned at different concentrations(200,400,600 mg·kg-1),once a day.The activities of AChE and catalase(CAT) and the levels of lipid peroxidation(LPO) of the organs were measured at 4,8,12 and 16 days.The results showed that(1)the activities of AChE were inhibited and showed an evident(dependence) on dose and time;(2)the activities of CAT in liver and kidney changed evidently and the levels of LPO were obvious;(3)after treated 16 days,the levels of LPO of spleen increased at the concentration of 600 mg·kg-1;(4)in brain and serum,the activities of catalase(CAT) increased first and then decreased following the PCNB concentrations varied from low to high.But the levels of LPO were not serious.PCNB could have nerve toxicity and oxidation damage on liver and kidney.
- pentachloronitrobenzene /
- acetylcholinesterase /
- oxidation damage /
- catalase /
- lipid peroxidation /
- mouse

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宋莉莉, 金明华, 刘晓梅, 袁星, 邓铁柱. 五氯硝基苯对小鼠脑AChE的影响及多器官氧化损伤研究[J]. 环境化学, 2006, 25(4): 462-465.

引用本文:

宋莉莉, 金明华, 刘晓梅, 袁星, 邓铁柱. 五氯硝基苯对小鼠脑AChE的影响及多器官氧化损伤研究[J]. 环境化学, 2006, 25(4): 462-465.

Citation:

五氯硝基苯对小鼠脑AChE的影响及多器官氧化损伤研究

1. 东北师范大学城市与环境科学学院, 长春, 130024;
2. 吉林大学公共卫生学院, 长春, 130021

收稿日期: 2005-09-01

基金项目:

教育部科学技术研究重点项目(03058)

关键词:

摘要: 测定五氯硝基苯(PCNB)对小鼠脑乙酰胆碱酯酶(AChE)的影响及多器官的氧化损伤作用.对昆明种小鼠采用灌胃法进行200,400和600 mg·kg-1三个剂量染毒,每日一次,分别于染毒4,8,12和16d测定AChE活性、过氧化氢酶(CAT)活性和脂质过氧化(LPO)产物丙二醛(MDA)的含量.结果表明:(1)小鼠脑AChE活性受到抑制,并表现为明显的时间和剂量依赖性;(2)肝、肾CAT活性变化显著,脂质过氧化作用明显;(3)高剂量毒物在染毒16d时对脾脏脂质过氧化作用明显增强;(4)脑和血清CAT活性随染毒时间延长先升高后降低,脂质过氧化作用不明显.PCNB对小鼠可能具有神经毒性,对肝、肾脏的氧化损伤作用较强.

English Abstract

STUDY ON THE ACTIVITY OF ACETYLCHOLINESTERASE AND OXIDATION DAMAGE ON DIFFERENT ORGANS OF MICE INDUCED BY PENTACHLORONITROBENZENE

1. College of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China;
2. Public Hygiene College, Jilin University, Changchun, 130021, China

Received Date: 2005-09-01

Fund Project:

Keywords:

Abstract: The effects of pentachloronitrobenzene(PCNB) on the activity of acetylcholinesterase(AChE) in brain and oxidation damage in various organs(brain,liver,kidney,spleen and serum) of mice were studied.The mice were poisoned at different concentrations(200,400,600 mg·kg-1),once a day.The activities of AChE and catalase(CAT) and the levels of lipid peroxidation(LPO) of the organs were measured at 4,8,12 and 16 days.The results showed that(1)the activities of AChE were inhibited and showed an evident(dependence) on dose and time;(2)the activities of CAT in liver and kidney changed evidently and the levels of LPO were obvious;(3)after treated 16 days,the levels of LPO of spleen increased at the concentration of 600 mg·kg-1;(4)in brain and serum,the activities of catalase(CAT) increased first and then decreased following the PCNB concentrations varied from low to high.But the levels of LPO were not serious.PCNB could have nerve toxicity and oxidation damage on liver and kidney.

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五氯硝基苯对小鼠脑AChE的影响及多器官氧化损伤研究

STUDY ON THE ACTIVITY OF ACETYLCHOLINESTERASE AND OXIDATION DAMAGE ON DIFFERENT ORGANS OF MICE INDUCED BY PENTACHLORONITROBENZENE

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五氯硝基苯对小鼠脑AChE的影响及多器官氧化损伤研究

English Abstract

STUDY ON THE ACTIVITY OF ACETYLCHOLINESTERASE AND OXIDATION DAMAGE ON DIFFERENT ORGANS OF MICE INDUCED BY PENTACHLORONITROBENZENE

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