Keywords: 
• SCCPs /
• Hep G2 /
• metabolites /
• amino acid metabolism /
• fatty acid metabolism

Abstract: Short chain chlorinated paraffins(SCCPs,C_10-13) are a large and complex group of polychlorinated n-alkanes,and ubiquitously found in the environment. However,very limited information is available for their toxicity mechanism,limiting the evaluation of their health risks. In this study,liquid chromatography-tandem mass spectrometry(LC-MS/MS) was adopted to investigate the influence of SCCPs exposure with different doses(0,1.0,10.0 and 100.0 μg·L^-1; C₁₃ -CPs; 55.0% Cl) on the metabolism of glucose,amino acids and fatty acids in human hepatoma Hep G2 cells. The result of partial least squares discriminant analysis(PLS-DA) showed that all SCCPs exposure groups were clearly distinct from the control group,indicating a significant metabolic perturbation inducedby SCCPs. Low-dose SCCPs exposure promoted the import of extracellular amino acids into cells. Compared with the control group,the contents of glutamine,tryptophan and serine in the culture medium of low-dose SCCPs exposure group(1.0 μg·L^-1) were significantly decreased(P<0.05). However,the high-dose SCCPs exposure(100.0 μg·L^-1) inhibited the transport of amino acids and glucose into cells,and significantly up-regulated the biosynthesis of lactic acid,alanine and cysteine. The disorder of amino acids metabolism would inevitably affect the protein biosynthesis. Meanwhile,SCCPs perturbed the metabolism of unsaturated fatty acids,and markedly up-regulated the contents of polyunsaturated fatty acids. In order to make sure the mode of action of SCCPs,transcriptomic and proteomic evidences on SCCPs toxicity should be further provided.