2种烷基多环芳烃对仿刺参CYP450和p53基因表达的影响研究
Effects of 2 Kinds of alkyl-PAHs on the Expression of CYP450 and p53 Genes of Apostichopus japonicus
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摘要: 海洋环境中多环芳烃类(PAHs)主要来源于海洋溢油事故以及沿海石油化工企业的废水排放，国内外大量研究发现海洋中的多环芳烃对海洋生物造成了潜在的生态风险。为了揭示不同浓度多环芳烃类污染物对海参的生态毒理效应，将仿刺参(Apostichopus japonicus)分别暴露于不同浓度的2种烷基多环芳烃3-甲基菲(5、10、100 μg·L-1)和2-甲基蒽(5、10、50 μg·L-1)中，检测暴露3 d、7 d和14 d后，3-甲基菲和2-甲基蒽胁迫下仿刺参CYP450和p53基因的相对表达量。结果表明，3-甲基菲和2-甲基蒽胁迫下，仿刺参CYP450和p53基因的表达均对毒物产生了不同程度的响应。与对照组相比，3-甲基菲各处理组对仿刺参CYP450和p53基因的表达均产生显著的抑制作用(P<0.05)；2-甲基蒽各处理组对仿刺参CYP450和p53基因的表达影响作用不同，暴露7 d后，2-甲基蒽各处理组对仿刺参CYP450基因的表达表现出抑制作用，对p53基因的表达表现出诱导作用。相同浓度与时间胁迫下，2-甲基蒽对仿刺参CYP450和p53基因表达的影响比3-甲基菲的影响大。上述研究结果表明，3-甲基菲和2-甲基蒽均可不同程度影响仿刺参CYP450和p53基因的表达，且与3-甲基菲相比，2-甲基蒽对仿刺参CYP450和p53基因表达的影响较明显。上述结果为多环芳烃类污染物对仿刺参的生物毒性评价提供了基础数据。Abstract: Marine organisms are facing a risk of exposure to a wide variety of toxic chemicals, such as polycyclic aromatic hydrocarbons (PAHs) from oil spill incidents and discharge of petrochemical wastewater. Besides, this kind of environmental pollutants pose a threat to the sea farming. As such, Apostichopus japonicus was exposed to 3-methylphenanthrene (5, 10 and 100 μg·L-1) and 2-methylanthracene(5, 10 and 50 μg·L-1) to study the toxic effects of alkyl-PAHs on the marine animals. Relative expression levels of CYP450 and p53 genes of Apostichopus japonicus under the stress of 3-methylphenanthrene and 2-methylanthracene were measured after 3 d, 7 d and 14 d. Results showed that 3-methylphenanthrene had significant inhibitory effect (P<0.05) on the expression of both CYP450 and p53 genes, while 2-methylanthracene had a different effect on the expression of CYP450 and p53 genes. From 7th day onwards, CYP450 gene expression was inhibited by 2-methylanthracene, but p53 gene expression was induced. Meanwhile, Apostichopus japonicus were more sensitive to 2-methylanthracene than 3-methylphenanthrene within the same concentration range and exposure duration. These results indicated that 3-methylphenanthrene and 2-methylanthracene could affect the expression of CYP450 and p53 genes of Apostichopus japonicus, and compared with 3-methylphenanthrene, 2-methylanthracene has significant effect on the expression of CYP450 and p53 genes. These results provide the basic data for the biotoxicity evaluation of PAHs pollutants on Apostichopus japonicus.
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Key words:
- 3-methylphenanthrene /
- 2-methylanthracene /
- Apostichopus japonicus /
- CYP450 /
- p53 /
- gene expression
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