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鼠李糖脂浓度对铜绿假单胞菌NY3烃代谢过程中细胞生理特性影响作用

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胡睿, 聂麦茜, 黄璐, 朱洪胜, 聂红云. 鼠李糖脂浓度对铜绿假单胞菌NY3烃代谢过程中细胞生理特性影响作用[J]. 环境工程学报, 2017, 11(4): 2591-2597. doi: 10.12030/j.cjee.201602049
引用本文: 胡睿, 聂麦茜, 黄璐, 朱洪胜, 聂红云. 鼠李糖脂浓度对铜绿假单胞菌NY3烃代谢过程中细胞生理特性影响作用[J]. 环境工程学报, 2017, 11(4): 2591-2597. doi: 10.12030/j.cjee.201602049
shu
HU Rui, NIE Maiqian, HUANG Lu, ZHU Hongsheng, NIE Hongyun. Effects of rhamnolipid concentration on cell physiological property of strain Pseudomonas aeruginosa NY3 in metabolic process of hydrocarbon[J]. Chinese Journal of Environmental Engineering, 2017, 11(4): 2591-2597. doi: 10.12030/j.cjee.201602049
Citation: HU Rui, NIE Maiqian, HUANG Lu, ZHU Hongsheng, NIE Hongyun. Effects of rhamnolipid concentration on cell physiological property of strain Pseudomonas aeruginosa NY3 in metabolic process of hydrocarbon[J]. Chinese Journal of Environmental Engineering, 2017, 11(4): 2591-2597. doi: 10.12030/j.cjee.201602049
shu

鼠李糖脂浓度对铜绿假单胞菌NY3烃代谢过程中细胞生理特性影响作用

  • 1.

    西安建筑科技大学环境与市政工程学院, 西安 710055

  • 基金项目:

    国家自然科学基金资助项目（51278405）

    陕西省重点科技创新团队建设计划（2013KCT-13）

  • 中图分类号: X172

Effects of rhamnolipid concentration on cell physiological property of strain Pseudomonas aeruginosa NY3 in metabolic process of hydrocarbon

  • 1.

    School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China

  • Fund Project:

  • 摘要: 为了研究表面活性剂鼠李糖脂（Rha）促进铜绿假单胞菌NY3烃降解与该菌细胞生理特性变化规律的相关性，在实验室可控条件下，研究了投加Rha后对该菌在整个培养过程中生长情况、正十六烷降解率、胞内脂多糖含量、胞内外蛋白含量等生理特性的影响。结果表明，NY3菌对正十六烷的降解率不受限于该鼠李糖脂临界胶束浓度（CMC=35 mg·L-1），即在投加高于CMC浓度时同样可以提高正十六烷的降解率，并增大体系生物量和积累大量酸性物质（多为烃的氧化产物），该酸性物质能被NY3菌利用并快速生长。投加Rha可增加细胞内脂多糖的含量，并与体系中被去除的烃类物质的量成正比；同时能促进胞内蛋白在短时间内增加并趋于稳定，而降解体系中胞外蛋白同样对于正十六烷的降解有积极作用。因此，投加Rha不仅能提高对疏水性有机物的增溶性，同时对代谢过程中菌体的生理生化特性有明显的改变，从而提高NY3菌对烃类物质的降解率。
    Abstract: Hydrocarbon degradation and changes in the physiological properties of Pseudomonas aeruginosa NY3 cells were studied by measuring cell growth, the hexadecane degradation rate, and contents of intracellular lipopolysaccharides and proteins as well as extracellular proteins throughout the fermentation process after adding rhamnolipid under laboratory controlled conditions. The results showed that the degradation rate of hexadecane could be increased when rhamnolipid was administered at a concentration higher than the critical micelle concentration (CMC=35 mg·L-1); additionally, biomass increased in the degradation system and there were dramatic increases in the accumulation of hydrocarbon oxidation products, which could be utilized by the NY3 cells for rapid growth. The intracellular lipopolysaccharide content increased following the addition of rhamnolipid and the increase was proportional to the amount of hydrocarbons removed; furthermore, intracellular proteins increased and were stabilized simultaneously. The presence of extracellular proteins had a positive effect on hexadecane degradation. Therefore, rhamnolipid not only improved the solubilization of hydrophobic organic compounds, but also obviously changed the physiological and biochemical characteristics of the P. aeruginosa NY3 strain during the metabolic process, which improved the efficiency of hydrocarbon biodegradation.
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出版历程
  • 收稿日期:  2016-03-24
  • 刊出日期:  2017-04-22
胡睿, 聂麦茜, 黄璐, 朱洪胜, 聂红云. 鼠李糖脂浓度对铜绿假单胞菌NY3烃代谢过程中细胞生理特性影响作用[J]. 环境工程学报, 2017, 11(4): 2591-2597. doi: 10.12030/j.cjee.201602049
引用本文: 胡睿, 聂麦茜, 黄璐, 朱洪胜, 聂红云. 鼠李糖脂浓度对铜绿假单胞菌NY3烃代谢过程中细胞生理特性影响作用[J]. 环境工程学报, 2017, 11(4): 2591-2597. doi: 10.12030/j.cjee.201602049
shu
HU Rui, NIE Maiqian, HUANG Lu, ZHU Hongsheng, NIE Hongyun. Effects of rhamnolipid concentration on cell physiological property of strain Pseudomonas aeruginosa NY3 in metabolic process of hydrocarbon[J]. Chinese Journal of Environmental Engineering, 2017, 11(4): 2591-2597. doi: 10.12030/j.cjee.201602049
Citation: HU Rui, NIE Maiqian, HUANG Lu, ZHU Hongsheng, NIE Hongyun. Effects of rhamnolipid concentration on cell physiological property of strain Pseudomonas aeruginosa NY3 in metabolic process of hydrocarbon[J]. Chinese Journal of Environmental Engineering, 2017, 11(4): 2591-2597. doi: 10.12030/j.cjee.201602049
shu

鼠李糖脂浓度对铜绿假单胞菌NY3烃代谢过程中细胞生理特性影响作用

  • 1. 西安建筑科技大学环境与市政工程学院, 西安 710055
  • 收稿日期:  2016-03-24
基金项目:

国家自然科学基金资助项目（51278405）

陕西省重点科技创新团队建设计划（2013KCT-13）

摘要: 为了研究表面活性剂鼠李糖脂（Rha）促进铜绿假单胞菌NY3烃降解与该菌细胞生理特性变化规律的相关性，在实验室可控条件下，研究了投加Rha后对该菌在整个培养过程中生长情况、正十六烷降解率、胞内脂多糖含量、胞内外蛋白含量等生理特性的影响。结果表明，NY3菌对正十六烷的降解率不受限于该鼠李糖脂临界胶束浓度（CMC=35 mg·L-1），即在投加高于CMC浓度时同样可以提高正十六烷的降解率，并增大体系生物量和积累大量酸性物质（多为烃的氧化产物），该酸性物质能被NY3菌利用并快速生长。投加Rha可增加细胞内脂多糖的含量，并与体系中被去除的烃类物质的量成正比；同时能促进胞内蛋白在短时间内增加并趋于稳定，而降解体系中胞外蛋白同样对于正十六烷的降解有积极作用。因此，投加Rha不仅能提高对疏水性有机物的增溶性，同时对代谢过程中菌体的生理生化特性有明显的改变，从而提高NY3菌对烃类物质的降解率。

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