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周媛, 周子洋, 封莉, 程翔, 张立秋. 高浓度发酵废水生产单细胞蛋白的菌种筛选[J]. 环境工程学报, 2016, 10(11): 6307-6312. doi: 10.12030/j.cjee.201506130
引用本文: 周媛, 周子洋, 封莉, 程翔, 张立秋. 高浓度发酵废水生产单细胞蛋白的菌种筛选[J]. 环境工程学报, 2016, 10(11): 6307-6312. doi: 10.12030/j.cjee.201506130
shu
ZHOU Yuan, ZHOU Ziyang, FENG Li, CHENG Xiang, ZHANG Liqiu. Strains screening for producing single cell protein from high concentration fermentation wastewater[J]. Chinese Journal of Environmental Engineering, 2016, 10(11): 6307-6312. doi: 10.12030/j.cjee.201506130
Citation: ZHOU Yuan, ZHOU Ziyang, FENG Li, CHENG Xiang, ZHANG Liqiu. Strains screening for producing single cell protein from high concentration fermentation wastewater[J]. Chinese Journal of Environmental Engineering, 2016, 10(11): 6307-6312. doi: 10.12030/j.cjee.201506130
shu

高浓度发酵废水生产单细胞蛋白的菌种筛选

  • 1.

    北京林业大学环境科学与工程学院, 北京 100083

  • 基金项目:

    国家水体污染治理与控制科技重大专项（2013ZX07201007-003）

Strains screening for producing single cell protein from high concentration fermentation wastewater

  • 1.

    College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China

  • Fund Project:

  • 摘要: 为了回收高浓度发酵废水中的有用资源，以近平滑假丝酵母（C. parapsilosis）、热带假丝酵母（C. tropicalis）、产朊假丝酵母（C. utilis）、汉逊德巴利酵母（D. hansenli）、酿酒酵母（S. cerevisiae）、皮状丝孢酵母（T. cutaneum）、白地霉（G. candidum）和黑曲霉（A. niger）8种常见工业菌种为研究对象，通过摇瓶发酵，考察了不同菌种利用高浓度发酵废水生产单细胞蛋白（SCP）的能力，同时比较废水灭菌和不灭菌两种条件对SCP产量的影响。结果表明，在废水COD浓度69 600 mg·L-1、TN浓度4 048 mg·L-1、初始pH 6.5、28℃、150 r·min-1、灭菌条件下发酵40 h，C. parapsilosis的菌体生物量和粗蛋白含量最高，可达7.04 g·L-1和1.87 g·L-1，对废水中COD和TN的去除率分别为33.5%和20.1%；C. parapsilosis和G. candidum可作为废水生产SCP并高效回收氮素和有机质的优势菌种。在不灭菌条件下，各菌种生产SCP的能力明显高于灭菌条件，且不同菌种产量差异不大，生物量和粗蛋白含量平均高达8.0 g·L-1和4.0 g·L-1，废水COD和TN的去除率分别为47%和33%。
    Abstract: In order to recover valuable resources from high-concentration fermentation wastewater, the single cell protein (SCP) producing ability of strains using molasses fermentation wastewater were investigated. The study employed eight fungal strains commonly used in industrial applications:Candida spp. (parapsilosis, tropicalis, utilis), D. hansenli,S. cerevisiae, T. cutaneum, G. candidum and A. niger. Flask fermentation experiments were used to study the SCP-producing ability of the various strains, and the effect of sterilization on SCP production. Wastewater with initial COD and TN of 69 600 mg·L-1 and 4 048 mg·L-1, respectively, was fermented under sterile conditions of pH 6.5, temperature 28℃, and 150 r·min-1 for 40 h. The results showed that C. parapsilosis had the highest biomass dry weight (7.04 g·L-1) and protein content (1.87 g·L-1), and achieved COD and TN removal rates of 33.5% and 20.1%, respectively. C. parapsilosis and G. candidum could be used as the predominant strains for SCP production as well as nitrogen and organic matter high efficient recovery from wastewater. When fermentation wastewater was pretreated without sterilization, the SCP production of strains was significantly higher than when sterilized. In addition, no significant differences in SCP production were observed between strains. The average biomass dry weight, protein content, COD, and TN removal of strains were as high as 8.0 g·L-1, 4.0 g·L-1, 47%, and 33%, respectively.
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  • [1] 高靖伟, 赫婷婷, 程翔, 等. EGSB-MBR组合工艺处理糖蜜发酵废水效能研究. 中国环境科学, 2015, 35(5): 1416-1422 GAO J., HE T., CHENG X., et al. Performance of the combined EGSB-MBR reactor treating fermentation wastewater. China Environmental Science, 2015, 35(5): 1416-1422(in Chinese)
    [2] Peihong, HAN Fei, SU Shuquan, et al. Using pig manure to promote fermentation of sugarcane molasses alcohol wastewater and its effects on microbial community structure. Bioresource Technology, 2014,155:323-329
    [3] 周玉, 贠建民, 宋德龙. 马铃薯淀粉废水SCP发酵菌种筛选及发酵条件优化. 食品工业, 2013(3): 20-23 ZHOU Y., YUN J., SONG D. Singl cell protein strains screening and fermentation conditions optimization by using potato stach wastwater. Food Industry, 2013(3): 20-23(in Chinese)
    [4] 李建政, 张平, 鲍立新, 等. 大豆乳清废水发酵生产单细胞蛋白的酵母. 哈尔滨工业大学学报, 2009, 41(2): 48-52 LI J., ZHANG P., BAO L., et al. Yeasts for single-cell protein by soybean whey fermentation. Journal of Harbin Insitute of Technology, 2009, 41(2): 48-52(in Chinese)
    [5] 曾鸿鹄, 姚毅, 朱义年, 等. 糖蜜废液生产单细胞蛋白的研究. 饲料研究, 2007(9): 5-8 ZENG H., YAO Y., ZHU Y., et al. Study on single cell protein from molesses fermentation wastewater. Feed Rearch, 2007,(9):5-8(in Chinese)
    [6] 李小雨. 多菌种发酵玉米浸泡水和糖蜜生产单细胞蛋白. 哈尔滨工业大学硕士学位论文, 2010 LI X. Producing single cell protein by yeasts fermentation of corn steep water and molasses. Harbin: Master Qissertationof Harbin Insitute of Technology, 2010
    [7] ZIINO M., Lo CURTO R.B., SALVO F., et al. Lipid composition of Geotrichum candidum single cell protein grown in continuous submerged culture. Bioresource Technology, 1999, 67(1):7-11
    [8] GARCÍA-GARIBAY M., GÓMEZ-RUIZ L., CRUZ-GUERRERO A. E.,et al. Single cell protein: Yeasts and bacteria. Encyclopedia of Food Microbiology, 2014: 431-438
    [9] NIGAM J. N. Single cell protein from pineapple cannery effluent. World Journal of Microbiology Biotechnology, 1998, 14: 693-696
    [10] NASSERI A. T., RASOUL-AMINI S., MOROWVAT M. H., et al. Single cell protein: Producton and process. American Journal of Food Technology, 2011, 6(2): 103-116
    [11] NIGAM P., VOGEL M. Bioconversion of sugar industry by-products-molasses and sugar beet pulp for single cell protein production by yeasts. Biomass and Bioenergy, 1991, 1(6): 339-345
    [12] 任克宁, 张福元, 王莹莹, 等. 白腐及黑曲霉对玉米秸秆生物降解的研究. 中国饲料, 2011(3): 35-38 REN K., ZHANG F. Y., WANG Y. Y., et al. Study on degradation of con straw by Phanerochaete chysosporium and Aspergillus niger. China Feed, 2011(3): 35-38(in Chinese)
    [13] 国家环境保护总局. 水和废水监测分析方法.4版. 北京: 中国环境科学出版社, 2002
    [14] 张健, 张唯广, 贠健民. 啤酒废水SCP发酵菌种的筛选及其发酵条件初探. 广州食品工业科技, 2002, 18(2): 26-28 ZHANG J., ZHANG W. G., YUN J. M. An observation of screening strains and culturing conditions for SCP fermentation in the wastewater of brewery. Guangzhou Food Science and Technology, 2002, 18(2): 26-28(in Chinese)
    [15] 王军, 郝新艳, 殷朝敏, 等. 用苎麻生物脱胶废水生产热带假丝酵母单细胞蛋白. 化工环保, 2012, 32(3): 255-259 WANG Jun, HAO Xinyan, YIN Chaomin, et al. Production of single cell protein from ramie bio-degumming wastewater using Candida tropicalis. Environmental Protection of Chemical Industry, 2012, 32(3): 255-259(in Chinese)
    [16] 李玉, 李其九, 尹依婷, 等. 白地霉净化和资源化玉米淀粉工业废水. 辽宁大学学报自然科学版, 2008, 35(1): 81-84 LI Y., LI Q., YI Y., et al. The experimental condition of purification and being resources of wastewater from maize starch processng by Geotrichum Candidum. Journal of Liaoning University,2008,35(1): 81-84(in Chinese)
    [17] 付源, 李建政, 鲍立新, 等. 大豆蛋白废水生产单细胞蛋白的菌种选择及其培养条件. 中国科技博览, 2007, 25(11): 9-13 FU Y., LI J., BAO L. X., et al. Selection of yeasts and the culture conditions for single cell protein production from soy protein wastewater by fermentation. Science & Technology Review, 2007, 25(11): 9-13(in Chinese)
    [18] 富中菲. 酵母单细胞蛋白固态发酵系统中氮素变化的研究. 吉林农业大学学报, 2002, 24(1): 92-97 FU Zhongfei. Study on the nitrogen-metabolism of single cell protein production in SSF (solid state fermentation). Journal of Jilin Agricultural University, 2002,24(1):92-97(in Chinese)
    [19] 鹿玉琼. 米曲霉处理马铃薯淀粉废水的研究. 兰州: 兰州大学, 2011 LU Y. Q. Starch processing wastewater in submerged cultures of Aspergillus oryzae. Lanzhou: Lanzhou University, 2011(in Chinese)
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  • 收稿日期:  2015-09-01
  • 刊出日期:  2016-11-26
周媛, 周子洋, 封莉, 程翔, 张立秋. 高浓度发酵废水生产单细胞蛋白的菌种筛选[J]. 环境工程学报, 2016, 10(11): 6307-6312. doi: 10.12030/j.cjee.201506130
引用本文: 周媛, 周子洋, 封莉, 程翔, 张立秋. 高浓度发酵废水生产单细胞蛋白的菌种筛选[J]. 环境工程学报, 2016, 10(11): 6307-6312. doi: 10.12030/j.cjee.201506130
shu
ZHOU Yuan, ZHOU Ziyang, FENG Li, CHENG Xiang, ZHANG Liqiu. Strains screening for producing single cell protein from high concentration fermentation wastewater[J]. Chinese Journal of Environmental Engineering, 2016, 10(11): 6307-6312. doi: 10.12030/j.cjee.201506130
Citation: ZHOU Yuan, ZHOU Ziyang, FENG Li, CHENG Xiang, ZHANG Liqiu. Strains screening for producing single cell protein from high concentration fermentation wastewater[J]. Chinese Journal of Environmental Engineering, 2016, 10(11): 6307-6312. doi: 10.12030/j.cjee.201506130
shu

高浓度发酵废水生产单细胞蛋白的菌种筛选

  • 1. 北京林业大学环境科学与工程学院, 北京 100083
  • 收稿日期:  2015-09-01
基金项目:

国家水体污染治理与控制科技重大专项（2013ZX07201007-003）

摘要: 为了回收高浓度发酵废水中的有用资源，以近平滑假丝酵母（C. parapsilosis）、热带假丝酵母（C. tropicalis）、产朊假丝酵母（C. utilis）、汉逊德巴利酵母（D. hansenli）、酿酒酵母（S. cerevisiae）、皮状丝孢酵母（T. cutaneum）、白地霉（G. candidum）和黑曲霉（A. niger）8种常见工业菌种为研究对象，通过摇瓶发酵，考察了不同菌种利用高浓度发酵废水生产单细胞蛋白（SCP）的能力，同时比较废水灭菌和不灭菌两种条件对SCP产量的影响。结果表明，在废水COD浓度69 600 mg·L-1、TN浓度4 048 mg·L-1、初始pH 6.5、28℃、150 r·min-1、灭菌条件下发酵40 h，C. parapsilosis的菌体生物量和粗蛋白含量最高，可达7.04 g·L-1和1.87 g·L-1，对废水中COD和TN的去除率分别为33.5%和20.1%；C. parapsilosis和G. candidum可作为废水生产SCP并高效回收氮素和有机质的优势菌种。在不灭菌条件下，各菌种生产SCP的能力明显高于灭菌条件，且不同菌种产量差异不大，生物量和粗蛋白含量平均高达8.0 g·L-1和4.0 g·L-1，废水COD和TN的去除率分别为47%和33%。

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