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任连海, 刘慧, 张明露. Cupriavidus necator (DSM428)利用地沟油合成PHA的条件优化[J]. 环境工程学报, 2016, 10(6): 3166-3172. doi: 10.12030/j.cjee.201501051
引用本文: 任连海, 刘慧, 张明露. Cupriavidus necator (DSM428)利用地沟油合成PHA的条件优化[J]. 环境工程学报, 2016, 10(6): 3166-3172. doi: 10.12030/j.cjee.201501051
shu
Ren Lianhai, Liu Hui, Zhang Minglu. Optimizing fermentation conditions for polyhydroxyalkanoates synthesis from waste oil by Cupriavidus necator (DSM428)[J]. Chinese Journal of Environmental Engineering, 2016, 10(6): 3166-3172. doi: 10.12030/j.cjee.201501051
Citation: Ren Lianhai, Liu Hui, Zhang Minglu. Optimizing fermentation conditions for polyhydroxyalkanoates synthesis from waste oil by Cupriavidus necator (DSM428)[J]. Chinese Journal of Environmental Engineering, 2016, 10(6): 3166-3172. doi: 10.12030/j.cjee.201501051
shu

Cupriavidus necator (DSM428)利用地沟油合成PHA的条件优化

  • 1.

    北京工商大学食品学院, 北京 100048

  • 基金项目:

    国家科技支撑计划课题(2012BAC25B01,2014BAC27B01-03)

    国家自然科学基金面上项目(51578008)

  • 中图分类号: X705

Optimizing fermentation conditions for polyhydroxyalkanoates synthesis from waste oil by Cupriavidus necator (DSM428)

  • 1.

    School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China

  • Fund Project:

  • 摘要: 聚羟基脂肪酸酯(PHA)是一种在细胞体内合成的天然高分子生物材料,由于其具有可降解性,因此可以代替传统塑料,已被广泛应用于生产生活中。选用Cupriavidus necator (DSM428)作为实验菌株,利用价格相对低廉的地沟油作为细菌生长的碳源,考察了pH、温度、培养时间、C/N(质量比)对PHA产生量的影响,同时对其结构及热学性质进行分析。通过实验发现,PHA最佳合成条件是pH值为8,温度为30℃,培养时间为84 h,C/N为20:1.5,PHA最大产量范围为7~9 g/L。
    Abstract: Polyhydroxyalkanoates (PHAs) are natural biopolymers that are produced intracellularly. PHA has been considered a strong candidate to replace petrochemical plastics owing to its biodegradation properties, and it is used widely in various products and applications. Waste oil has been used as an economical, low-cost carbon source for PHA synthesis. Cupriavidus necator (DSM428) was used as an experimental bacterium to examine the influence of pH, temperature, incubation time, and C/N ratio on PHA concentration. In addition, the structural and thermal properties were analyzed. The optimal fermentation conditions were as follows: pH 8; fermentation temperature, 30℃; 20:1.5 C/N ratio; and incubation time, 84 h. The maximum concentration of PHA ranged from 7 to 9 g/L.
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出版历程
  • 收稿日期:  2015-02-16
  • 刊出日期:  2016-06-03
任连海, 刘慧, 张明露. Cupriavidus necator (DSM428)利用地沟油合成PHA的条件优化[J]. 环境工程学报, 2016, 10(6): 3166-3172. doi: 10.12030/j.cjee.201501051
引用本文: 任连海, 刘慧, 张明露. Cupriavidus necator (DSM428)利用地沟油合成PHA的条件优化[J]. 环境工程学报, 2016, 10(6): 3166-3172. doi: 10.12030/j.cjee.201501051
shu
Ren Lianhai, Liu Hui, Zhang Minglu. Optimizing fermentation conditions for polyhydroxyalkanoates synthesis from waste oil by Cupriavidus necator (DSM428)[J]. Chinese Journal of Environmental Engineering, 2016, 10(6): 3166-3172. doi: 10.12030/j.cjee.201501051
Citation: Ren Lianhai, Liu Hui, Zhang Minglu. Optimizing fermentation conditions for polyhydroxyalkanoates synthesis from waste oil by Cupriavidus necator (DSM428)[J]. Chinese Journal of Environmental Engineering, 2016, 10(6): 3166-3172. doi: 10.12030/j.cjee.201501051
shu

Cupriavidus necator (DSM428)利用地沟油合成PHA的条件优化

  • 1. 北京工商大学食品学院, 北京 100048
  • 收稿日期:  2015-02-16
基金项目:

国家科技支撑计划课题(2012BAC25B01,2014BAC27B01-03)

国家自然科学基金面上项目(51578008)

摘要: 聚羟基脂肪酸酯(PHA)是一种在细胞体内合成的天然高分子生物材料,由于其具有可降解性,因此可以代替传统塑料,已被广泛应用于生产生活中。选用Cupriavidus necator (DSM428)作为实验菌株,利用价格相对低廉的地沟油作为细菌生长的碳源,考察了pH、温度、培养时间、C/N(质量比)对PHA产生量的影响,同时对其结构及热学性质进行分析。通过实验发现,PHA最佳合成条件是pH值为8,温度为30℃,培养时间为84 h,C/N为20:1.5,PHA最大产量范围为7~9 g/L。

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