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活性污泥胞外聚合物提取方法比较与热碱法优化

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谢丹瑜, 康得军, 唐虹, 吴捷, 邱福杰. 活性污泥胞外聚合物提取方法比较与热碱法优化[J]. 环境工程学报, 2016, 10(9): 5295-5300. doi: 10.12030/j.cjee.201412239
引用本文: 谢丹瑜, 康得军, 唐虹, 吴捷, 邱福杰. 活性污泥胞外聚合物提取方法比较与热碱法优化[J]. 环境工程学报, 2016, 10(9): 5295-5300. doi: 10.12030/j.cjee.201412239
shu
XIE Danyu, KANG Dejun, TANG Hong, WU Jie, QIU Fujie. Extraction of extracellular polymeric substances from activated sludge and optimization of thermal-alkaline method[J]. Chinese Journal of Environmental Engineering, 2016, 10(9): 5295-5300. doi: 10.12030/j.cjee.201412239
Citation: XIE Danyu, KANG Dejun, TANG Hong, WU Jie, QIU Fujie. Extraction of extracellular polymeric substances from activated sludge and optimization of thermal-alkaline method[J]. Chinese Journal of Environmental Engineering, 2016, 10(9): 5295-5300. doi: 10.12030/j.cjee.201412239
shu

活性污泥胞外聚合物提取方法比较与热碱法优化

  • 1.

    福州大学土木工程学院, 福州 350116

  • 2.

    福州市环境监测中心站, 福州 350011

  • 基金项目:

    福州市科技计划项目（2014-G-58）

    福州大学人才基金资助项目（XRC-1268）

    福州大学科技发展基金资助项目（2013-XY-23）

    福州大学贵重仪器设备开放测试基金（2016T040）

  • 中图分类号: X703

Extraction of extracellular polymeric substances from activated sludge and optimization of thermal-alkaline method

  • 1.

    College of Civil Engineering, Fuzhou University, Fuzhou 350116, China

  • 2.

    Fuzhou Environmental Monitoring Centre, Fuzhou 350011, China

  • Fund Project:

  • 摘要: 为比较不同方法对曝气池活性污泥胞外聚合物（EPS）的提取效果，采用6种方法对EPS进行提取。结果表明：热碱法对EPS的提取量最大，提取总量为64.95 mg·g-1；其次是NaOH法、CER法和加热法，超声法和硫酸法对EPS的提取效果不佳，提取量低于10 mg·g-1；在所提取的EPS中，多糖含量高于蛋白质。为研究pH、水浴温度和时间对热碱法提取效果的影响，采用正交实验对热碱法的提取条件进行优化，确定热碱法提取EPS的适宜条件为水浴温度80℃，加热时间10 min，pH 13，该条件下蛋白质和多糖的提取量达到最大，分别为29.55 mg·g-1和33.37 mg·g-1。
    Abstract: The extraction methods of six different extracellular polymeric substances (EPS) were studied on activated sludge sampled from an aeration tank. Results showed that the thermal-alkaline method gave the highest extraction values (64.95 mg·g-1), followed by sodium hydroxide, a cation exchange resin, and the thermal method, while ultrasonication and the sulfuric acid method resulted in relatively low extraction values (below 10 mg·g-1). In addition, the extracted EPS contained more carbohydrates than proteins. To optimize the extraction conditions of the thermal-alkaline method, the effect of pH, water bath temperature, and water bath time on the extraction were investigated through an orthogonal experiment. The optimum conditions were determined, as follows:pH of 13, water bath temperature of 80℃, and water bath time of 10 min. The highest extraction values in terms of proteins (29.55 mg·g-1) and carbohydrates (33.37 mg·g-1) were achieved under the optimum conditions.
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  • 收稿日期:  2015-03-03
  • 刊出日期:  2016-09-10
谢丹瑜, 康得军, 唐虹, 吴捷, 邱福杰. 活性污泥胞外聚合物提取方法比较与热碱法优化[J]. 环境工程学报, 2016, 10(9): 5295-5300. doi: 10.12030/j.cjee.201412239
引用本文: 谢丹瑜, 康得军, 唐虹, 吴捷, 邱福杰. 活性污泥胞外聚合物提取方法比较与热碱法优化[J]. 环境工程学报, 2016, 10(9): 5295-5300. doi: 10.12030/j.cjee.201412239
shu
XIE Danyu, KANG Dejun, TANG Hong, WU Jie, QIU Fujie. Extraction of extracellular polymeric substances from activated sludge and optimization of thermal-alkaline method[J]. Chinese Journal of Environmental Engineering, 2016, 10(9): 5295-5300. doi: 10.12030/j.cjee.201412239
Citation: XIE Danyu, KANG Dejun, TANG Hong, WU Jie, QIU Fujie. Extraction of extracellular polymeric substances from activated sludge and optimization of thermal-alkaline method[J]. Chinese Journal of Environmental Engineering, 2016, 10(9): 5295-5300. doi: 10.12030/j.cjee.201412239
shu

活性污泥胞外聚合物提取方法比较与热碱法优化

  • 1. 福州大学土木工程学院, 福州 350116
  • 2. 福州市环境监测中心站, 福州 350011
  • 收稿日期:  2015-03-03
基金项目:

福州市科技计划项目（2014-G-58）

福州大学人才基金资助项目（XRC-1268）

福州大学科技发展基金资助项目（2013-XY-23）

福州大学贵重仪器设备开放测试基金（2016T040）

摘要: 为比较不同方法对曝气池活性污泥胞外聚合物（EPS）的提取效果，采用6种方法对EPS进行提取。结果表明：热碱法对EPS的提取量最大，提取总量为64.95 mg·g-1；其次是NaOH法、CER法和加热法，超声法和硫酸法对EPS的提取效果不佳，提取量低于10 mg·g-1；在所提取的EPS中，多糖含量高于蛋白质。为研究pH、水浴温度和时间对热碱法提取效果的影响，采用正交实验对热碱法的提取条件进行优化，确定热碱法提取EPS的适宜条件为水浴温度80℃，加热时间10 min，pH 13，该条件下蛋白质和多糖的提取量达到最大，分别为29.55 mg·g-1和33.37 mg·g-1。

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