高级搜索

黑藻根际对沉积物中氨氧化细菌和古菌的影响

,  , 

downloadPDF

上一篇

下一篇

李倩, 田翠翠, 肖邦定. 黑藻根际对沉积物中氨氧化细菌和古菌的影响[J]. 环境工程学报, 2014, 8(10): 4209-4214.
引用本文: 李倩, 田翠翠, 肖邦定. 黑藻根际对沉积物中氨氧化细菌和古菌的影响[J]. 环境工程学报, 2014, 8(10): 4209-4214.
shu
Li Qian, Tian Cuicui, Xiao Bangding. Effect of Hydrilla verticillata rhizosphere on ammonia oxidizing bacteria and archaea in sediment[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4209-4214.
Citation: Li Qian, Tian Cuicui, Xiao Bangding. Effect of Hydrilla verticillata rhizosphere on ammonia oxidizing bacteria and archaea in sediment[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4209-4214.
shu

黑藻根际对沉积物中氨氧化细菌和古菌的影响

  • 1.

    中国科学院水生生物研究所, 武汉 430072

  • 2.

    中国科学院大学, 北京 100049

  • 3.

    中国科学院藻类生物学重点实验室, 武汉 430072

  • 基金项目:

    国家“水体污染控制与治理”科技重大专项课题（2013ZX07102005）

  • 中图分类号: Q945.1;S154.3

Effect of Hydrilla verticillata rhizosphere on ammonia oxidizing bacteria and archaea in sediment

  • 1.

    Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

  • 2.

    University of Chinese Academy of Sciences, Beijing 100049, China

  • 3.

    Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

  • Fund Project:

  • 摘要: 氨氧化反应对水生态系统氮循环和氮的去除有重要作用，沉水植物通过根系泌氧促进沉积物中硝化反应并对氨氧化细菌和古菌的分布产生影响。本研究以轮叶黑藻为实验对象，利用微电极研究沉积物-水界面的溶解氧变化，研究了黑藻根系对沉积物中氨氧化细菌和古菌数量的影响。结果表明，黑藻通过根系泌氧增加沉积物-水界面的溶解氧量和表层沉积物有氧层厚度，有氧层厚度增加了3 mm以上；种植黑藻后，根际沉积物中氨氧化细菌数量逐渐增加，氨氧化古菌数量前30天增加随后减少，氨氧化细菌与氨氧化古菌amoA基因拷贝数的比值由0.51增加到6.75，说明黑藻根际沉积物更适宜氨氧化细菌的生存。
    Abstract: Ammonia oxidation plays an improtant role in the cycling and removal of nitrogen in aquatic ecosystems.Oxygen releases from the roots of macrophyte stimulates nitrification in the rhizosphere and thus affects the distribution of rhizosphere-associated ammonia-oxidizing bacteria (AOB) and archaea (AOA) in sediment.In the present study,microelectrodes and real-time PCR were used to investigate the changes in dissolved oxygen in sediment-water interface and quantities of AOA and AOB in sediment colonized with the submerged macrophyte Hydrilla verticillata.The results showed that H.verticillata increased the contents of dissolved oxygen in sediment-water interface and oxic layer thickness in sediment surface.More than 3 mm of oxic surface layer was found in vegetated sediment compared with unvegetated sediment.H.verticillata could increase the number of AOB,while the number of AOA increased at first 30 days but then gradually decreased to initial level.The ratios of the bacterial-to-archaeal amoA gene increased from 0.51 to 6.75 in the rhizosphere,indicating that the rhizosphere of H.verticillata provided conditions favoring enrichment of AOB.
  • 加载中
  • [1] Xia X.H.,Li S.Z.,Shen Z.Y.Effect of nitrification on nitrogen flux across sediment-water interface.Water Environment Research,2008,80(11):2175-2182
    [2] 张娟,李春华,叶春,等.沉水植物黑藻腐解对微囊藻休眠体复苏的影响.环境科学研究,2012,25(1):43-50 Zhang Juan,Li Chunhua,Ye Chun,et al.Effects of Hydrilla verticillata decomposition on recruitment of the dormant Microcystis spp.Research of Environmental Sciences,2012,25(1):43-50(in Chinese)
    [3] 薛维纳,彭岩波,宋祥甫,等.弱光胁迫对黑藻生理生化特性的影响.安徽农业科学,2012,40(7):4169-4172 Xue Weina,Peng Yanbo,Song Xiangpu,et al.Effects of light intensity on physiological and biochemical characteristics of Hydrilla verticillata (L.f.) Royle.Journal of Anhui Agricutural Sciences,2012,40(7):4169-4172(in Chinese)
    [4] Trias R.,Ruiz-Rueda O.,García-Lledó A.,et al.Emergent macrophytes act selectively on ammonia-oxidizing bacteria and archaea.Applied & Environmental Microbiology,2012,78(17):6352-6356
    [5] Wei B.,Yu X.,Zhang S.T.,et al.Comparison of the community structures of ammonia-oxidizing bacteria and archaea in rhizoplanes of floating aquatic macrophytes.Microbiological Research,2011,166(6):468-474
    [6] Ai C.,Liang G.Q.,Sun J.W.,et al.Different roles of rhizosphere effect and long-term fertilization in the activity and community structure of ammonia oxidizers in a calcareous fluvo-aquic soil.Soil Biology and Biochemistry,2013,57:30-42
    [7] 董莲华,杨金水,袁红莉.氨氧化细菌的分子生态学研究进展.应用生态学报,2008,19(6):1381-1388 Dong Lianhua,Yang Jinshui,Yuan Hongli.Research advances in molecular ecology of ammonia oxidizing bacteria.Chinese Journal of Applied Ecology,2008,19(6):1381-1388(in Chinese)
    [8] Schleper C.,Jurgens G.,Jonuscheit M.Genomic studies of uncultivated archaea.Nature Reviews Microbiology,2005,3(6):479-488
    [9] Wang Y.F.,Feng Y.Y.,Ma X.J.,et al.Seasonal dynamics of ammonia/ammonium-oxidizing prokaryotes in oxic and anoxic wetland sediments of subtropical coastal mangrove.Appl.Microbiol.Biotechnol.,2013,97:7919-7934
    [10] Beman J.M.,Popp B.N.,Francis C.A.Molecular and biogeochemical evidence for ammonia oxidation by marine Crenarchaeota in the Gulf of California.The ISME Journal,2008,2(4):429-441
    [11] Vandesompele J.,Preter K.D.,Pattyn F.,et al.Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.Genome Biology,2002,3(7):1-11
    [12] 张甘霖,龚子同.土壤调查实验室分析方法.北京:科学出版社,2012
    [13] Rotthauwe J.H.,Witzel K.P.,Liesack W.The ammonia monooxygenase structural gene amoA as a functional maker:Molecular fine-scale analysis of natural ammonia- oxidizing populations.Applied & Environmental Microbiology,1997,63(12):4704-4712
    [14] Francis C.A.,Roberts K.J.,Beman J.M.,et al.Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean.Proceedings of the National Academy of the Sciences of the United States of America,2005,102(41):14683-14688
    [15] 王素梅,潘伟斌,黄华.富营养化水体中光照对黑藻生长的影响.水资源保护,2010,26(2):53-55,91 Wang Sumei,Pan Weibin,Huanghua.Effect of light on growth of Hydrilla verticillata in eutrophic water.Water Resources Protection,2010,26(2):53-55,91(in Chinese)
    [16] 吴娟,吴振斌,成水平.黑藻对水体和沉积物理化性质的改善和营养元素的去除作用.水生生物学报,2009,33(4):589-595 Wu Juan,Wu Zhenbin,Cheng Shuiping.Effect of Hydrilla verticillata on characteristics of water and sediment and removals of nutrition.Acta Hydrobiologica Sinica,2009,33(4):589-595(in Chinese)
    [17] 马晓磊,徐继荣,张德民,等.城市内河强还原性沉积物耗氧及相关因素研究.环境科学研究,2010,23(12):1499-1505 Ma Xiaolei,Xu Jirong,Zhang Demin,et al.Research on sediment oxygen demand and related factors for strong reductive sediments in urban rivers.Research of Environmental Sciences,2010,23(12):1499-1505(in Chinese)
    [18] 邓泓,叶志鸿,黄铭洪.湿地植物根系泌氧的特征.华东师范大学学报(自然科学版),2007,(6):69-76 Deng Hong,Ye Zhihong,Huang Minghong.Characteristics of radial oxygen loss from root of wetland plants.Journal of East China Normal University (Natural Science),2007,(6):69-76(in Chinese)
    [19] Rueda O.R.,Hallin S.,Baneras L.Structure and function of denitrifying and nitrifying bacterial communities in relation to the plant species in a constructed wetland.FEMS Microbiology Ecology,2009,67(2):308-319
    [20] Pitcher A.,Villanueva L.,Hopmans E.C.,et al.Niche segregation of ammonia-oxidizing archaea and anammox bacteria in the Arabian Sea oxygen minimum zone.The ISME Journal,2011,5(12):1896-1904
    [21] Hatzenpichler R.Diversity,physiology,and niche differentiation of ammonia-oxidizing archaea.Applied & Environmental Microbiology,2012,78(21):7501-7510
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  1941
  • HTML全文浏览数:  1390
  • PDF下载数:  805
  • 施引文献:  0
出版历程
  • 收稿日期:  2013-12-05
  • 刊出日期:  2014-09-28
李倩, 田翠翠, 肖邦定. 黑藻根际对沉积物中氨氧化细菌和古菌的影响[J]. 环境工程学报, 2014, 8(10): 4209-4214.
引用本文: 李倩, 田翠翠, 肖邦定. 黑藻根际对沉积物中氨氧化细菌和古菌的影响[J]. 环境工程学报, 2014, 8(10): 4209-4214.
shu
Li Qian, Tian Cuicui, Xiao Bangding. Effect of Hydrilla verticillata rhizosphere on ammonia oxidizing bacteria and archaea in sediment[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4209-4214.
Citation: Li Qian, Tian Cuicui, Xiao Bangding. Effect of Hydrilla verticillata rhizosphere on ammonia oxidizing bacteria and archaea in sediment[J]. Chinese Journal of Environmental Engineering, 2014, 8(10): 4209-4214.
shu

黑藻根际对沉积物中氨氧化细菌和古菌的影响

  • 1.  中国科学院水生生物研究所, 武汉 430072
  • 2.  中国科学院大学, 北京 100049
  • 3.  中国科学院藻类生物学重点实验室, 武汉 430072
  • 收稿日期:  2013-12-05
基金项目:

国家“水体污染控制与治理”科技重大专项课题（2013ZX07102005）

摘要: 氨氧化反应对水生态系统氮循环和氮的去除有重要作用，沉水植物通过根系泌氧促进沉积物中硝化反应并对氨氧化细菌和古菌的分布产生影响。本研究以轮叶黑藻为实验对象，利用微电极研究沉积物-水界面的溶解氧变化，研究了黑藻根系对沉积物中氨氧化细菌和古菌数量的影响。结果表明，黑藻通过根系泌氧增加沉积物-水界面的溶解氧量和表层沉积物有氧层厚度，有氧层厚度增加了3 mm以上；种植黑藻后，根际沉积物中氨氧化细菌数量逐渐增加，氨氧化古菌数量前30天增加随后减少，氨氧化细菌与氨氧化古菌amoA基因拷贝数的比值由0.51增加到6.75，说明黑藻根际沉积物更适宜氨氧化细菌的生存。

English Abstract

    全文HTML

参考文献 (21)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心