高级搜索

印染废水接触氧化池中悬浮污泥和生物膜的微生物群落比较分析

,  ,  ,  , 

downloadPDF

上一篇

下一篇

黄俊, 宋吟玲, 王学华, 杜雨露, 吴鹏. 印染废水接触氧化池中悬浮污泥和生物膜的微生物群落比较分析[J]. 环境工程学报, 2014, 8(8): 3241-3246.
引用本文: 黄俊, 宋吟玲, 王学华, 杜雨露, 吴鹏. 印染废水接触氧化池中悬浮污泥和生物膜的微生物群落比较分析[J]. 环境工程学报, 2014, 8(8): 3241-3246.
shu
Huang Jun, Song Yinling, Wang Xuehua, Du Yulu, Wu Peng. Analysis of the microbial community differences between suspending sludge and biofilm in contact-oxidation tank for dyeing wastewater treatment[J]. Chinese Journal of Environmental Engineering, 2014, 8(8): 3241-3246.
Citation: Huang Jun, Song Yinling, Wang Xuehua, Du Yulu, Wu Peng. Analysis of the microbial community differences between suspending sludge and biofilm in contact-oxidation tank for dyeing wastewater treatment[J]. Chinese Journal of Environmental Engineering, 2014, 8(8): 3241-3246.
shu

印染废水接触氧化池中悬浮污泥和生物膜的微生物群落比较分析

  • 1.

    苏州科技学院环境科学与工程学院, 苏州 215009

  • 2.

    江苏省环境科学与工程重点实验室, 苏州 215009

  • 基金项目:

    江苏省高校自然科学基金资助项目（13KJB610014）

  • 中图分类号: X703

Analysis of the microbial community differences between suspending sludge and biofilm in contact-oxidation tank for dyeing wastewater treatment

  • 1.

    School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China

  • 2.

    Jiangsu Provincial Key Laboratory of Environmental Science and Engineering, Suzhou 215009, China

  • Fund Project:

  • 摘要: 为了揭示接触氧化池中悬浮污泥和生物膜菌群群落结构的异同，提取基因组DNA，采用PCR扩增与454高通量测序技术对两者菌群测序并进行了操作单元（OTU）聚类分析、多样性分析和分类学分析。结果表明，悬浮污泥和生物膜菌群的高通量测序分析分别得到优化序列8 256条和10 592条，测序覆盖深度都在80%左右，经过97%相似度归并后分别得到570个OTUs和694个OTUs，菌群多样性分析显示悬浮污泥内菌群的丰度和多样性都低于生物膜，悬浮污泥有356个属的细菌构成，而生物膜内则有387个，都归属于21个门。悬浮污泥和生物膜内不仅存在共有菌种，也含有各自的特定菌种，说明不同的微环境使其有了相对应的优势群落。通过这种差异的比较分析，更能准确地了解接触氧化池中微生物的群落组成情况，有利于分析其与系统功能的关系。
    Abstract: PCR amplification and 454 high-throughput sequencing from extracted genomic DNA were employed to reveal the similarity and difference between suspended microbial communities and attached ones in the contact-oxidation tank. The operational units hierarchical clustering, diversity and taxonomy analysis were also conducted. The dominant sequences from high-throughput sequencing were 8 256 and 10 592 for suspended and attached communities, respectively, and the coverage was around 80%. 570 and 694 OTUs(operational taxonomic units)were obtained through 97% similarity combination. The community diversity analysis showed that the richness and diversity of suspended communities were lower than those of attached ones. The suspended microbial was composed of 356 bacterial genera, with the attached of 387 kinds. All belonged to 21 phyla. The suspended and attached microbial shared the same species, however, their own the specific ones, respectively. It indicated that micro-environment caused the corresponding dominant communities. Finally, the relationship between the microbial community structure and its functions may be elucidated by such different analysis.
  • 加载中
  • [1] 奚旦立, 马春燕. 印染废水的分类、组成及性质. 印染, 2010, 36(14):51-53 Xi D. L., Ma C. Y. Classification, composition and properties of dyeing effluents. Dyeing & Finishing, 2010, 36(14):51-53(in Chinese)
    [2] Hye O. P., Sanghwa O., Rabindra B., et al. Application of A2O moving-bed biofilm reactors for textvile dyeing wastewater treatment. Korean Journal of Chemical Engineering, 2010, 27(3):893-899
    [3] Tang Z. G., Liu C., Chen Y., et al. Batch and fixed-bed adsorption of actual dyeing wastewater on sludge-based adsorbent from pyrolusite-catalyzed pyrolysis. Fresenius Environmental Bulletin, 2010, 19(12a):3000-3006
    [4] 高廷耀, 顾国维, 周琪. 水污染控制工程. 北京: 高等教育出版社, 2011
    [5] Mardis E. R. The impact of next-generation sequencing technology on genetics. Trends in Genetics, 2008, 24(3):133-141
    [6] Ansorge W. J. Next-generation DNA sequencing techniques. New Biotechnology, 2009, 25(4):195-203
    [7] 吕昌勇, 陈朝银, 葛锋, 等. 微生物分子生态学研究方法的新进展. 中国生物工程杂志, 2012, 32(8):111-118 Lü C. Y., Chen C. Y., Ge F., et al. The new development of the research method for molecular microbial ecology. China Biotechnology, 2012, 32(8):111-118(in Chinese)
    [8] 李亮, 魏桂芳, 童耕雷, 等. 用PCR指纹图技术分析焦化废水接触氧化池中悬浮污泥和生物膜的微生物种群组成. 中国微生态学杂志, 2004, 16(1):8-9 Li L., Wei G. F., Tong G. L., et al. Analysis of the microbial community differences between suspending sludge and biofilm in oxidation tank for coking wastewater treatment with PCR fingerprinting technique. Chinese Journal of Microecology, 2004, 16(1):8-9(in Chinese)
    [9] Turnbaugh P. J., Hamady M., Yatsunenko T., et al. A core gut microbiome in obese and lean twins. Nature, 2009, 457(7228):480-484
    [10] Kennedy D. M., Stanton J. A., García J. A., et al. Microbial analysis of bite marks by sequence comparison of streptococcal DNA. PLoS One, 2012, 7(12):e51757
    [11] Quast C., Pruesse E., Yilmaz P., et al. The SILVA ribosomal RNA gene database project:Improved data processing and web-based tools. Nucleic Acids Research, 2013, 41(D1):D590-D596
    [12] Schloss P. D., Westcott S. L., Ryabin T., et al. Introducing mothur:open-source, platform-independent, community-supported software for describing and comparing microbial communities. Applied Environmental Microbiology, 2009, 75(23):7537-7541
    [13] 蒙嵘, 浦跃武, 任敦建, 等. PCR-DGGE技术分析染整废水微生物群落多样性. 生物技术通报, 2012, (10):137-141 Meng R., Pu Y. W., Ren D. J., et al. Application of PCR-DGGE to analysis of microbial community diversity in dyeing wastewater. Biotechnology Bulletin, 2012, (10):137-141(in Chinese)
    [14] 吴佳佳, 张盾, 陈士强. 硫酸盐还原菌及其典型代谢产物对溶解氧还原反应的影响. 中国腐蚀电化学及测试方法专业委员会2012学术年会论文集, 2012 Wu J. J., Zhang D., Chen S. Q. Influence of sulphate-reducing bacteria and their typical metabolites on dissolved oxygen reduction reaction, 2012 National Conference on Corrosion Electrochemistry and Measuring Methods, 2012(in Chinese)
    [15] 曹新垲, 杨琦, 郝春博. 厌氧污泥降解萘动力学与生物多样性研究. 环境科学, 2012, 33(10):3535-3541 Cao X. K., Yang Q., Hao C. B. Degradation kinetics of naphthalene by anaerobic sludge and analysis of the bacterial biodiversity. Environmental Science, 2012, 33(10):3535-3541(in Chinese)
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  1512
  • HTML全文浏览数:  862
  • PDF下载数:  1108
  • 施引文献:  0
出版历程
  • 收稿日期:  2014-05-06
  • 刊出日期:  2014-07-31
黄俊, 宋吟玲, 王学华, 杜雨露, 吴鹏. 印染废水接触氧化池中悬浮污泥和生物膜的微生物群落比较分析[J]. 环境工程学报, 2014, 8(8): 3241-3246.
引用本文: 黄俊, 宋吟玲, 王学华, 杜雨露, 吴鹏. 印染废水接触氧化池中悬浮污泥和生物膜的微生物群落比较分析[J]. 环境工程学报, 2014, 8(8): 3241-3246.
shu
Huang Jun, Song Yinling, Wang Xuehua, Du Yulu, Wu Peng. Analysis of the microbial community differences between suspending sludge and biofilm in contact-oxidation tank for dyeing wastewater treatment[J]. Chinese Journal of Environmental Engineering, 2014, 8(8): 3241-3246.
Citation: Huang Jun, Song Yinling, Wang Xuehua, Du Yulu, Wu Peng. Analysis of the microbial community differences between suspending sludge and biofilm in contact-oxidation tank for dyeing wastewater treatment[J]. Chinese Journal of Environmental Engineering, 2014, 8(8): 3241-3246.
shu

印染废水接触氧化池中悬浮污泥和生物膜的微生物群落比较分析

  • 1.  苏州科技学院环境科学与工程学院, 苏州 215009
  • 2.  江苏省环境科学与工程重点实验室, 苏州 215009
  • 收稿日期:  2014-05-06
基金项目:

江苏省高校自然科学基金资助项目（13KJB610014）

摘要: 为了揭示接触氧化池中悬浮污泥和生物膜菌群群落结构的异同，提取基因组DNA，采用PCR扩增与454高通量测序技术对两者菌群测序并进行了操作单元（OTU）聚类分析、多样性分析和分类学分析。结果表明，悬浮污泥和生物膜菌群的高通量测序分析分别得到优化序列8 256条和10 592条，测序覆盖深度都在80%左右，经过97%相似度归并后分别得到570个OTUs和694个OTUs，菌群多样性分析显示悬浮污泥内菌群的丰度和多样性都低于生物膜，悬浮污泥有356个属的细菌构成，而生物膜内则有387个，都归属于21个门。悬浮污泥和生物膜内不仅存在共有菌种，也含有各自的特定菌种，说明不同的微环境使其有了相对应的优势群落。通过这种差异的比较分析，更能准确地了解接触氧化池中微生物的群落组成情况，有利于分析其与系统功能的关系。

English Abstract

    全文HTML

参考文献 (15)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心