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一株高效硝化菌的鉴定及其特性

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邓斌, 郑佳佳, 傅罗琴, 张小平, 梁权, 沈文英, 李卫芬. 一株高效硝化菌的鉴定及其特性[J]. 环境工程学报, 2013, 7(9): 3635-3641.
引用本文: 邓斌, 郑佳佳, 傅罗琴, 张小平, 梁权, 沈文英, 李卫芬. 一株高效硝化菌的鉴定及其特性[J]. 环境工程学报, 2013, 7(9): 3635-3641.
shu
Deng Bin, Zheng Jiajia, Fu Luoqin, Zhang Xiaoping, Liang Quan, Shen Wenying, Li Weifen. Identification and characteristics of a high-efficient nitrification bacterium[J]. Chinese Journal of Environmental Engineering, 2013, 7(9): 3635-3641.
Citation: Deng Bin, Zheng Jiajia, Fu Luoqin, Zhang Xiaoping, Liang Quan, Shen Wenying, Li Weifen. Identification and characteristics of a high-efficient nitrification bacterium[J]. Chinese Journal of Environmental Engineering, 2013, 7(9): 3635-3641.
shu

一株高效硝化菌的鉴定及其特性

  • 1.

    浙江大学动物科学学院农业部动物营养与饲料重点实验室, 杭州 310058

  • 2.

    绍兴文理学院生命科学学院, 绍兴 312000

  • 基金项目:

    国家"973"重点基础研究发展规划项目(2009CB118705)

    公益性行业(农业)科研专项(201203083)

    国家"水体污染控制与治理"科技重大专项(2012ZX07101-012-03)

  • 中图分类号: Q935

Identification and characteristics of a high-efficient nitrification bacterium

  • 1.

    Key Laboratory of Animal Nutrition & Feed Science, Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou 310058, China

  • 2.

    Department of Biology, Life Science College of Shaoxing University, Shaoxing 312000, China

  • Fund Project:

  • 摘要: 本研究通过测定菌株ABT01在不同初始氮浓度、pH、C/N、温度和溶氧条件下对氨氮的去除效果,获得该菌株的最佳应用条件。实验结果表明,当初始氨氮浓度低于40 mg/L时,该菌株的氨氮去除率高达85%以上。该菌株最适脱氨氮条件均为:pH 5.0~7.0、C/N=5、35℃、摇床转速150 r/min(溶解氧5.1 mg/L),氨氮去除率最高达96.8%。同时该菌株经16S rDNA测序、细胞壁脂肪酸组成等鉴定方法,确定 ABT01为枯草芽孢杆菌(Bacillus subtilis)。研究表明,枯草芽孢杆菌ABT01具有较好的氨氮去除能力,对水产养殖水质调控有潜在的应用价值。
    Abstract: In order to optimize the growth condition for the strain ABT01, we evaluated the ammonium removal efficiencies with different initial ammonium concentrations, pH, C/N, temperature and DO. The observed ammonium removal rate was more than 85% in 24 hours at an initial ammonium concentration below 40mg/L. The result showed that the best conditions for ammonium removal were as follows: pH 5.0~7.0, C/N=5, 35℃ and 150 r/min (DO is about 5.1 mg/L). Under the conditions, the highest ammonium removal rate could reach up to 96.8%. Through 16 S rDNA sequencing and fatty acid composition analysis of cell wall, the strain was identified as Bacillus subtilis. The results indicated that ABT01 had a potential role in ammonium removal in aquaculture practice.
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  • 收稿日期:  2012-08-03
  • 刊出日期:  2013-09-15
邓斌, 郑佳佳, 傅罗琴, 张小平, 梁权, 沈文英, 李卫芬. 一株高效硝化菌的鉴定及其特性[J]. 环境工程学报, 2013, 7(9): 3635-3641.
引用本文: 邓斌, 郑佳佳, 傅罗琴, 张小平, 梁权, 沈文英, 李卫芬. 一株高效硝化菌的鉴定及其特性[J]. 环境工程学报, 2013, 7(9): 3635-3641.
shu
Deng Bin, Zheng Jiajia, Fu Luoqin, Zhang Xiaoping, Liang Quan, Shen Wenying, Li Weifen. Identification and characteristics of a high-efficient nitrification bacterium[J]. Chinese Journal of Environmental Engineering, 2013, 7(9): 3635-3641.
Citation: Deng Bin, Zheng Jiajia, Fu Luoqin, Zhang Xiaoping, Liang Quan, Shen Wenying, Li Weifen. Identification and characteristics of a high-efficient nitrification bacterium[J]. Chinese Journal of Environmental Engineering, 2013, 7(9): 3635-3641.
shu

一株高效硝化菌的鉴定及其特性

  • 1. 浙江大学动物科学学院农业部动物营养与饲料重点实验室, 杭州 310058
  • 2. 绍兴文理学院生命科学学院, 绍兴 312000
  • 收稿日期:  2012-08-03
基金项目:

国家"973"重点基础研究发展规划项目(2009CB118705)

公益性行业(农业)科研专项(201203083)

国家"水体污染控制与治理"科技重大专项(2012ZX07101-012-03)

摘要: 本研究通过测定菌株ABT01在不同初始氮浓度、pH、C/N、温度和溶氧条件下对氨氮的去除效果,获得该菌株的最佳应用条件。实验结果表明,当初始氨氮浓度低于40 mg/L时,该菌株的氨氮去除率高达85%以上。该菌株最适脱氨氮条件均为:pH 5.0~7.0、C/N=5、35℃、摇床转速150 r/min(溶解氧5.1 mg/L),氨氮去除率最高达96.8%。同时该菌株经16S rDNA测序、细胞壁脂肪酸组成等鉴定方法,确定 ABT01为枯草芽孢杆菌(Bacillus subtilis)。研究表明,枯草芽孢杆菌ABT01具有较好的氨氮去除能力,对水产养殖水质调控有潜在的应用价值。

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