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灵芝漆酶对直接蓝86的催化脱色性能

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赵世光, 刘强, 张庆庆, 汤明礼. 灵芝漆酶对直接蓝86的催化脱色性能[J]. 环境工程学报, 2013, 7(2): 765-770.
引用本文: 赵世光, 刘强, 张庆庆, 汤明礼. 灵芝漆酶对直接蓝86的催化脱色性能[J]. 环境工程学报, 2013, 7(2): 765-770.
shu
Zhao Shiguang, Liu Qiang, Zhang Qingqing, Tang Mingli. Decolorization of Direct Bule 86 catalyzed by laccase from Ganoderma lucidum[J]. Chinese Journal of Environmental Engineering, 2013, 7(2): 765-770.
Citation: Zhao Shiguang, Liu Qiang, Zhang Qingqing, Tang Mingli. Decolorization of Direct Bule 86 catalyzed by laccase from Ganoderma lucidum[J]. Chinese Journal of Environmental Engineering, 2013, 7(2): 765-770.
shu

灵芝漆酶对直接蓝86的催化脱色性能

  • 1.

    安徽工程大学生物与化学工程学院,芜湖 241000

  • 2.

    微生物发酵安徽省工程技术研究 中心,芜湖 241000

  • 3.

    中国科学院离子束生物工程学重点实验室,合肥 230031

  • 基金项目:

    安徽省高校省级自然科学研究项目(KJ2011B025)

    安徽省科技厅国际科技合作计划项目(10080703035)

    安徽工程大学引进人才科研基金(2007YQQ007)

  • 中图分类号: X703

Decolorization of Direct Bule 86 catalyzed by laccase from Ganoderma lucidum

  • 1.

    College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China

  • 2.

    Microorganism Fermentation Engineering and Technology Research Center, Wuhu 241000, China

  • 3.

    Key Laboratory of Ion Beam Bio-Engineering, Chinese Academy of Sciences, Hefei 230031, China

  • Fund Project:

  • 摘要: 利用灵芝菌Ganoderma lucidum U-281漆酶对直接蓝86进行酶促氧化脱色,并对其降解机理进行了探讨。结果表明,染料-漆酶共反应体系在20~50℃及pH小于5.0范围内,直接蓝86均可脱色50%以上;漆酶对直接蓝86具有宽泛的浓度适应性,对300 mg/L的该染料仍具有耐受性。最优脱色工艺参数为温度40℃、pH 5.0、染料初始浓度200 mg/L、漆酶用量1 U/mL。在优化条件下,直接蓝863 h的脱色率达到54.54%,48 h脱色率达到91.54%。紫外-可见吸收光谱分析表明,漆酶的酶促氧化导致染料的分子结构产生了变化,是造成直接蓝86脱色的主要发生机制。
    Abstract: The enzymatic oxydative decolorization of Direct Blue 86 by laccase from Ganoderma lucidum U-281 was studied. Besides, the degradation mechanism was discussed. The results show that in laccase-dye co-reaction system more than 50% of Direct Blue 86 is decolorized under conditions of 20℃ to 50℃ and pH value less than 5.0. Laccase has a wide range of adaptability towards the dye concentration and is endurable with 300 mg/L of Direct Blue 86. The optimum decoloration parameters are as follows: 200 mg/L of initial dye concentration, 1 U/mL of laccase dosage, 40℃ and pH 5.0 without rotation. Decoloration rates of Direct Blue 86 are 54.54% and 91.54%, respectively after 3 hours and 48 hours under the optimum conditions. UV-visible light spectrum analysis suggests that enzymatic oxidation results in changes of dye molecular structures, which is the primary mechanism causing decoloration of Direct Blue 86.
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出版历程
  • 收稿日期:  2012-02-06
  • 刊出日期:  2013-02-02
赵世光, 刘强, 张庆庆, 汤明礼. 灵芝漆酶对直接蓝86的催化脱色性能[J]. 环境工程学报, 2013, 7(2): 765-770.
引用本文: 赵世光, 刘强, 张庆庆, 汤明礼. 灵芝漆酶对直接蓝86的催化脱色性能[J]. 环境工程学报, 2013, 7(2): 765-770.
shu
Zhao Shiguang, Liu Qiang, Zhang Qingqing, Tang Mingli. Decolorization of Direct Bule 86 catalyzed by laccase from Ganoderma lucidum[J]. Chinese Journal of Environmental Engineering, 2013, 7(2): 765-770.
Citation: Zhao Shiguang, Liu Qiang, Zhang Qingqing, Tang Mingli. Decolorization of Direct Bule 86 catalyzed by laccase from Ganoderma lucidum[J]. Chinese Journal of Environmental Engineering, 2013, 7(2): 765-770.
shu

灵芝漆酶对直接蓝86的催化脱色性能

  • 1.  安徽工程大学生物与化学工程学院,芜湖 241000
  • 2.  微生物发酵安徽省工程技术研究 中心,芜湖 241000
  • 3.  中国科学院离子束生物工程学重点实验室,合肥 230031
  • 收稿日期:  2012-02-06
基金项目:

安徽省高校省级自然科学研究项目(KJ2011B025)

安徽省科技厅国际科技合作计划项目(10080703035)

安徽工程大学引进人才科研基金(2007YQQ007)

摘要: 利用灵芝菌Ganoderma lucidum U-281漆酶对直接蓝86进行酶促氧化脱色,并对其降解机理进行了探讨。结果表明,染料-漆酶共反应体系在20~50℃及pH小于5.0范围内,直接蓝86均可脱色50%以上;漆酶对直接蓝86具有宽泛的浓度适应性,对300 mg/L的该染料仍具有耐受性。最优脱色工艺参数为温度40℃、pH 5.0、染料初始浓度200 mg/L、漆酶用量1 U/mL。在优化条件下,直接蓝863 h的脱色率达到54.54%,48 h脱色率达到91.54%。紫外-可见吸收光谱分析表明,漆酶的酶促氧化导致染料的分子结构产生了变化,是造成直接蓝86脱色的主要发生机制。

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