甲苯在硝酸盐还原条件下的生物降解性能研究

刘秀丽; 赵林; 刘涉江

环境工程学报

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刘秀丽, 赵林, 刘涉江. 甲苯在硝酸盐还原条件下的生物降解性能研究[J]. 环境工程学报, 2011, 5(3): 644-648.

引用本文:

刘秀丽, 赵林, 刘涉江. 甲苯在硝酸盐还原条件下的生物降解性能研究[J]. 环境工程学报, 2011, 5(3): 644-648.

Liu Xiuli, Zhao Lin, Liu Shejiang. Study on biodegradation of toluene under nitrate reducing condition[J]. Chinese Journal of Environmental Engineering, 2011, 5(3): 644-648.

Citation:

Liu Xiuli, Zhao Lin, Liu Shejiang. Study on biodegradation of toluene under nitrate reducing condition[J]. Chinese Journal of Environmental Engineering, 2011, 5(3): 644-648.

甲苯在硝酸盐还原条件下的生物降解性能研究

1.
天津大学环境科学与工程学院，天津 300072
基金项目:
国家自然科学基金资助项目(50879052)

天津市自然科学基金资助项目（10JCYBJC05500）
中图分类号: X53

Study on biodegradation of toluene under nitrate reducing condition

1.
School of Environmental Sciences and Engineering, Tianjin 300072, China
Fund Project:

摘要: 通过对长期受石油污染的土著微生物进行培养驯化，得到适于降解甲苯的优势混合菌，并对其在硝酸盐还原条件下降解甲苯的性能进行研究。结果表明，驯化所得甲苯降解菌的最适降解条件为：温度25℃，pH值7.5，C/N值15，接种量1%。混合降解菌对硝态氮的去除符合一级动力学特征，其降解速率常数为0.0021 mg/（L·h）。采用非抑制型MONOD动力学方程，对甲苯降解过程进行拟合，得到混合菌的表观最大比增殖速率为0.0348 h-1,而甲苯的最大比基质降解速率为0.0225 h-1，半衰期为4.50 d。
- 甲苯 /
- 硝酸盐 /
- 缺氧降解 /
- MONOD动力学
Abstract: Mixed culture was acclimatized from soil contaminated by petroleum and applied on a series of batch experiments to investigate the anoxic toluene biodegradation with nitrate as electron acceptor. The results show that the optimal temperature is 25℃, pH is 7.5, C/N ratio is 15 and microbes density is 1%。Further study shows the removal of nitrate-nitrogen tallies with one order kinetic characteristics, which degradation rate constant is 0.0021 mg/（L·h）. Through MONOD kinetics analysis, the obvious maximum-specific-growth rate of mixed culture is 0.0348 h-1 and maximum specific-toluene-degradation rate is 0.0225 h-1 with half-life 4.50 d.
- toluene /
- nitrate /
- anoxic degradation /
- MONOD kinetics

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刘秀丽, 赵林, 刘涉江. 甲苯在硝酸盐还原条件下的生物降解性能研究[J]. 环境工程学报, 2011, 5(3): 644-648.

引用本文:

刘秀丽, 赵林, 刘涉江. 甲苯在硝酸盐还原条件下的生物降解性能研究[J]. 环境工程学报, 2011, 5(3): 644-648.

Liu Xiuli, Zhao Lin, Liu Shejiang. Study on biodegradation of toluene under nitrate reducing condition[J]. Chinese Journal of Environmental Engineering, 2011, 5(3): 644-648.

Citation:

Liu Xiuli, Zhao Lin, Liu Shejiang. Study on biodegradation of toluene under nitrate reducing condition[J]. Chinese Journal of Environmental Engineering, 2011, 5(3): 644-648.

甲苯在硝酸盐还原条件下的生物降解性能研究

1. 天津大学环境科学与工程学院，天津 300072

收稿日期: 2010-04-19

基金项目:

国家自然科学基金资助项目(50879052)

天津市自然科学基金资助项目（10JCYBJC05500）

关键词:

摘要: 通过对长期受石油污染的土著微生物进行培养驯化，得到适于降解甲苯的优势混合菌，并对其在硝酸盐还原条件下降解甲苯的性能进行研究。结果表明，驯化所得甲苯降解菌的最适降解条件为：温度25℃，pH值7.5，C/N值15，接种量1%。混合降解菌对硝态氮的去除符合一级动力学特征，其降解速率常数为0.0021 mg/（L·h）。采用非抑制型MONOD动力学方程，对甲苯降解过程进行拟合，得到混合菌的表观最大比增殖速率为0.0348 h-1,而甲苯的最大比基质降解速率为0.0225 h-1，半衰期为4.50 d。

English Abstract

Study on biodegradation of toluene under nitrate reducing condition

1. School of Environmental Sciences and Engineering, Tianjin 300072, China

Received Date: 2010-04-19

Fund Project:

Keywords:

Abstract: Mixed culture was acclimatized from soil contaminated by petroleum and applied on a series of batch experiments to investigate the anoxic toluene biodegradation with nitrate as electron acceptor. The results show that the optimal temperature is 25℃, pH is 7.5, C/N ratio is 15 and microbes density is 1%。Further study shows the removal of nitrate-nitrogen tallies with one order kinetic characteristics, which degradation rate constant is 0.0021 mg/（L·h）. Through MONOD kinetics analysis, the obvious maximum-specific-growth rate of mixed culture is 0.0348 h-1 and maximum specific-toluene-degradation rate is 0.0225 h-1 with half-life 4.50 d.

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甲苯在硝酸盐还原条件下的生物降解性能研究

Study on biodegradation of toluene under nitrate reducing condition

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甲苯在硝酸盐还原条件下的生物降解性能研究

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Study on biodegradation of toluene under nitrate reducing condition

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