亲水性多孔载体在流化床中的生物膜形成过程分析
Process of biofilm colonization of a hydrophilic porous carrier in fluidized bed reactor
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摘要: 采用实验制备的一种新型亲水性多孔聚合物作为流化床反应器中生物膜附着生长的载体，实现流态化水力条件下的生物挂膜过程。在3个结构尺寸相同的流化床反应器中考察了接种污泥浓度、进水有机负荷及载体粒径对亲水性多孔载体生物挂膜量的影响，试验结果表明，接种污泥浓度为30 g VSS/L、进水TOC值为350 mg/L、载体粒径为5～8 mm时载体表面的附着生物量最大，反应器运行12 d的载体附着生物量达到4.45 g VSS/L，膜结构稳定，表现出较活性污泥法更高的活性。在进水TOC、氨氮浓度分别为350 mg/L、50 mg/L，HRT为6 h的情况下，两者的去除率分别达到了97.1％和64.3%，表明载体上的生物膜对污水中TOC及氨氮的去除表现出高效率。挂膜后载体表面上的微生物以丝状菌为主，孔壁上的微生物以球菌和杆菌为主要生物相，证明载体内外表面皆适宜微生物的生长，并且形成合理的生物相分布。Abstract: As the carriers of the growth of biofilm in fluidized bed reactor, a new kind of hydrophilic porous carrier developed by our own group was used to implement the biofilm colonization in three fluidized bed reactors with the same identical structure. Experiments were conducted to evaluate the effects of hydrophilic porous carrier on biofilm formation by sludge inoculation, influent TOCloading and carrier size. The tests showed that when sludge inoculation was 30 g VSS/L, influent TOC loading 350 mg/L and carrier size 5～8 mm, biofilm on hydrophilic porous carriers was most, 4.45 g VSS/L 12 days after the reactor starting, showing satisfying performance. Attached rate of removal TOC and netrogen were 97.1％ and 64.3% with influent TOC 350 mg/L, nitrogen 50 mg/L and HRT 6.0 h. Scanning electron micrographs showed that filamentous bacteria were the main biota on surface of hydrophilic porous carriers, cocci and bacillus were the main biota on the keyhole wall in hydrophilic porous carriers. It proved that there were considerable amount of microorganisms varieties of microorganism species on & in the hydrophilic porous carriers and the amount of cell varied obviously with the position of the hydrophilic porous carriers.
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