基于免疫分析技术的雌二醇与壬基酚同时检测方法
Simultaneous detection of estradiol and nonylphenol based on immunosorbent assay
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摘要: 本文研究了基于间接竞争酶联免疫分析技术（ELISA）对两种典型内分泌干扰物（雌二醇，壬基酚）的同时检测方法.首先，建立了内分泌干扰物雌二醇和壬基酚单独检测的酶联免疫检测方法，分别得到了雌二醇和壬基酚酶联免疫检测方法的标准曲线.雌二醇的检测限为3.18 μg·L-1，定量检测区间为4.18-11.98 μg·L-1；壬基酚的检测限为24.78 μg·L-1，定量检测区间为36.51-363.43 μg·L-1.采用优化后的检测条件，进一步设计建立了两种内分泌干扰物的同时检测方法，并测定了清华大学自来水和荷塘水配制的雌二醇和壬基酚加标样品，所有样品的变异系数和回收率分别在2%-14%和85%-116%之间，表明该方法适用于实际水样中雌二醇和壬基酚的同时检测.Abstract: In this paper, two typical endocrine disruptors (estradiol, nonylphenol) were detected simultaneously based on indirect competitive enzyme-linked immunosorbent assay (ELISA). Firstly, the enzyme-linked immunosorbent assay for the detection of estradiol or nonylphenol lone was established seprarately. The standard curves of estradiol and nonylphenol was obtained:The detection limit of estradiol was 3.18 μg·L-1, and the quantitative detection range was 4.18-11.98 μg·L-1; The detection limit of nonylphenol was 24.78 μg·L-1, and the quantitative detection interval is 36.51-363.43 μg·L-1. Then, using the optimized detection conditions, a method for the simultaneous detection of the two endocrine disruptors was further designed, and the spiked samples of estradiol and nonylphenol in tap water and lotus pond water of Tsinghua University were determined. The coefficient of variation and recovery of all samples were 2%-14% and 85%-116%, indicating that the method is applicable for the simultaneous detection of estradiol and nonylphenol in real water samples.
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Key words:
- enzyme-linked immunosorbent assay /
- simultaneous detection /
- estradiol /
- nonylphenol
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