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利用大型溞评估离子液体[C8mim]Cl生物毒性初探

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李珊珊, 李萍萍, 陈彪, 薛春叶, 董京伟, 李斌, 邓祥元. 利用大型溞评估离子液体[C8mim]Cl生物毒性初探[J]. 环境化学, 2020, (11): 3024-3029. doi: 10.7524/j.issn.0254-6108.2019081602
引用本文: 李珊珊, 李萍萍, 陈彪, 薛春叶, 董京伟, 李斌, 邓祥元. 利用大型溞评估离子液体[C8mim]Cl生物毒性初探[J]. 环境化学, 2020, (11): 3024-3029. doi: 10.7524/j.issn.0254-6108.2019081602
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LI Shanshan, LI Pingping, CHEN Biao, XUE Chunye, DONG Jingwei, LI Bin, DENG Xiangyuan. Preliminary study on the biotoxicity evaluation of[C8mim]Cl by using Daphnia magna[J]. Environmental Chemistry, 2020, (11): 3024-3029. doi: 10.7524/j.issn.0254-6108.2019081602
Citation: LI Shanshan, LI Pingping, CHEN Biao, XUE Chunye, DONG Jingwei, LI Bin, DENG Xiangyuan. Preliminary study on the biotoxicity evaluation of[C8mim]Cl by using Daphnia magna[J]. Environmental Chemistry, 2020, (11): 3024-3029. doi: 10.7524/j.issn.0254-6108.2019081602
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利用大型溞评估离子液体[C8mim]Cl生物毒性初探

  • 江苏科技大学生物技术学院, 镇江, 212018

    • 通讯作者: 邓祥元, E-mail: dengxy2016@foxmail.com
  • 基金项目:

    江苏省第十五批"六大人才高峰"高层次人才选拔培养项目（SWYY-025）和镇江市重点研发计划项目（SH2019004）资助.

Preliminary study on the biotoxicity evaluation of[C8mim]Cl by using Daphnia magna

  • College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212018, China

    • Corresponding author: DENG Xiangyuan, dengxy2016@foxmail.com
  • Fund Project: Supported by the Six Talent Peaks Project in Jiangsu Province (SWYY-025) and Key Research & Development Project of Zhenjiang (SH2019004).

  • 摘要: 为评估1-辛基-3-甲基咪唑氯盐（[C8mim]Cl）的生物毒性，本研究以大型溞（Daphnia magna）为实验对象，通过急性毒性实验分析了不同日龄大型溞对[C8mim]Cl的敏感性，并以存活率、产溞能力和蜕皮次数为指标研究了[C8mim]Cl对大型溞的慢性毒性效应，这将为评估离子液体的毒性效应提供科学依据.结果表明，[C8mim]Cl对1、2、4日龄大型溞的48 h半致死浓度（LC50）分别为208.9、182.7、178.6 μg·L-1，表明随着大型溞日龄的增加，[C8mim]Cl对大型溞的毒性效应逐渐增大并趋于稳定.此外，[C8mim]Cl对大型溞生活史中的初次产溞时间、总产溞数目、总产溞次数和蜕皮次数等影响不显著，但显著影响大型溞的存活率和初次产溞个数，因此，大型溞的存活率和初次产溞个数是评估[C8mim]Cl对大型溞慢性毒性效应的两个较为敏感的指标.
    Abstract: In order to evaluate the biotoxicity of 1-octyl-3-methylimidazolium chloride ([C8mim]Cl) to Daphnia magna, the sensitivity of D. magna aged different days to[C8mim]Cl was studied through acute toxicity experiments. The survival rate, capacity of producing, and exuviate times were used as indicators to investigate the chronic toxic effects of[C8mim]Cl on D. magna, which would provide scientific evidences for further evaluating the toxic effects of ionic liquids. The results showed that the 48 h-LC50 values of[C8mim]Cl to D. magna aged 1, 2 and 4 days macrophages were 208.9, 182.7, 178.6 μg·L-1, respectively, indicating that the toxic effects of[C8mim]Cl on D. magna increased as the day-age D. magna increased and then stabilized. Moreover, it was showed that[C8mim]Cl had no significant effects on the first-generation time, total number and times, and total exuviate times of Daphnia magna, but the ionic liquid significantly inhibited the survival rate and the the first-generation number of Daphnia magna, suggesting that the survival rate and the first-generation number of Daphnia magna, would be the two sensitive indicators for evaluating the chronic toxic effects of[C8mim]Cl on D. magna.
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  • [1] KESKIN S, KAYRAK-TALAY D, AKMAN U, et al. A review of ionic liquids towards supercritical fluid applications[J]. The Journal of Supercritical Fluids, 2007, 43(1):150-180.
    [2] 李国平, 薛娟琴, 杨婷, 等.[Bmim] PF6离子液体支撑液膜迁移氰根的实验研究[J]. 环境化学, 2017, 36(6):1226-1235.

    LI G P, XUE J Q, YANG T, et al. Treatment of cyanide wastewater by[Bmim] PF6 supported ionic liquid membrane[J]. Environmental Chemistry, 2017, 36(6):1226-1235(in Chinese).

    [3] BUBALO M C, RADOŠEVIĆ K, REDOVNIKOVIĆ I R, et al. A brief overview of the potential environmental hazards of ionic liquids[J]. Ecotoxicology and Environmental Safety, 2014, 99:1-12.
    [4] 胡雪生, 余江, 夏寒松, 等. 离子液体的绿色合成及环境性质[J]. 化学通报, 2005, 68(12):906-910.

    HU X S, YU J, XIA H S, et al. Green synthesis and environmental properties of ionic liquids[J]. Chemistry (Huaxue Tongbao), 2005, 68(12):906-910(in Chinese).

    [5] PHAM T P T, CHO C W, YUN Y S. Environmental fate and toxicity of ionic liquids:A review[J]. Water Research, 2010, 44(2):352-372.
    [6] PAWŁOWSKA B, TELESIŃSKI A, BICZAK R. Phytotoxicity of ionic liquids[J]. Chemosphere, 2019, 237:124436.
    [7] SWATLOSKI R P, HOLBREY J D, MEMON S B, et al. Using Caenorhabditis elegans to probe toxicity of 1-alkyl-3-methylimidazolium chloride based ionic liquids[J]. Chemical Communications, 2004, 6:668-669.
    [8] PRETTI C, CHIAPPE C, PIERACCINI D, et al. Acute toxicity of ionic liquids to the zebrafish (Danio rerio)[J]. Green Chemistry, 2006, 8(3):238-240.
    [9] ZHANG C, ZHU L, WANG J, et al. The acute toxic effects of imidazolium-based ionic liquids with different alkyl-chain lengths and anions on zebrafish (Danio rerio)[J]. Ecotoxicology and Environmental Safety, 2017, 140:235-240.
    [10] JODYNIS-LIEBERT J, NOWICKI M, MURIAS M, et al. Cytotoxicity, acute and subchronic toxicity of ionic liquid, didecyldimethylammonium saccharinate, in rats[J]. Regulatory Toxicology and Pharmacology, 2010, 57(2/3):266-273.
    [11] 李效宇, 苗晓青, 皇培培, 等. 离子液体溴化-1-辛基-3-甲基咪唑诱发金鱼血红细胞微核和核异常的研究[J]. 安全与环境学报, 2010, 10(2):5-7.

    LI X Y, MIAO X Q, HUANG P P, et al. Micronuclei and nuclear anomalies in the erythrocytes of goldfish induced by ionic liquid 1-octyl-3-methylimidazolium bromide[J]. Journal of Safety and Environment, 2010, 10(2):5-7(in Chinese).

    [12] 刘建梅, 刘济宁, 陈英文, 等. 四溴双酚A和三溴苯酚对大型溞的急性和慢性毒性[J]. 环境科学学报, 2015, 35(6):1946-1954.

    LIU J M, LIU J N, CHEN Y W, et al. Acute and chronic toxicity of tetrabromobisphenol A and tribromophenol to Daphnia magna[J]. Acta Scientiae Circumstantiae, 2015, 35(6):1946-1954(in Chinese).

    [13] 张丽彬, 王金鑫, 王启山, 等. 浮游动物在生物操纵法除藻中的作用研究[J]. 生态环境, 2007, 16(6):1648-1653.

    ZHANG L B, WANG J X, WANG Q S, et al. Effect of zooplankton on removing algae in bio-manipulation[J]. Ecology and Environment, 2007, 16(6):1648-1653(in Chinese).

    [14] 蔡小宇, 姜锦林, 单正军, 等. 壬基酚聚氧乙烯醚对大型溞的急性和慢性毒性效应[J]. 生态与农村环境学报, 2018, 34(1):81-86.

    CAI X Y, JIANG J L, SHAN Z J, et al. Acute and chronic toxicity of nonylphenol ethoxylates to Daphnia magna[J]. Journal of Ecology and Rural Environment, 2018, 34(1):81-86(in Chinese).

    [15] 杨灿, 沈根祥, 胡双庆, 等. 氯霉素对大型溞的急性和慢性毒性效应研究[J]. 生态毒理学报, 2018, 13(5):248-255.

    YANG C, SHEN G X, HU S Q, et al. Acute and chronic toxicity of chloramphenicol to Daphnia magna[J]. Asian Journal of Ecotoxicology, 2018, 13(5):248-255(in Chinese).

    [16] 刘倩, 杜青平, 刘涛, 等. 纳米氧化锌致大型溞的毒性效应特征[J]. 环境科学学报, 2019, 39(4):1332-1339.

    LIU Q, DU Q P, LIU T, et al. Study on the toxicity effects of nanometer zinc oxide on Daphnia magna[J]. Acta Scientiae Circumstantiae, 2019, 39(4):1332-1339(in Chinese).

    [17] WANG S, MECKLING K A, MARCONE M F, et al. Synergistic, additive, and antagonistic effects of food mixtures on total antioxidant capacities[J]. Journal of Agricultural and Food Chemistry, 2011, 59(3):960-968.
    [18] OECD. Test Guideline No. 211. Effects on biotic systems test:Daphnia magna reproduction test[S]. Paris:OECD Publishing, 2008.
    [19] OECD. Test Guideline No. 211. Test guidelines for testing of chemicals:Daphnia magna reproduction test[S]. Paris:OECD Publishing, 1998.
    [20] STAUBER J L, FLORENCE T M. The effect of culture medium on metal toxicity to the marine diatom Nitzschia closterium and freshwater green alga Chlorella pyrenidosa[J]. Water Research, 1989, 23(7):907-911.
    [21] OECD. Test Guideline No. 202. Test guidelines for testing of chemicals:Daphnia sp. acute immobilization test[S]. Paris:OECD Publishing, 2004.
    [22] U.S. EPA. Guidelines for deriving numerical water quality criteria for the protection of aquatic organism and their uses[S]. Washington:National Technical Information Service, 1985.
    [23] FRANCIS P C, GROTHE D W, SCHEURING J C. Chronic toxicity of 4-nitrophenol to Daphnia magna straus under statis-renewal and flow-through conditions[J]. Bulletin of Environmental Contamination and Toxicology, 1986, 36(5):730-737.
    [24] 顾兵, 张政, 李玉萍, 等. 半数致死剂量及其计算方法概述[J]. 中国职业医学,2009, 36(6):507-508

    , 511. GU B, ZHANG Z, LI Y P, et al. Summary of LD50 and its calculation methods[J]. Chinese Occupational Medicine, 2009, 36(6):507-508, 511(in Chinese).

    [25] 李翠萍, 吴民耀, 王宏元. 3种半数致死浓度计算方法之比较[J]. 动物医学进展, 2012, 33(9):89-92.

    LI C P, WU M Y, WANG H Y. LC50 calculated by kochi, probit analysis and linear regression methods[J]. Progress in Veterinary Medicine, 2012, 33(9):89-92(in Chinese).

    [26] 张榜军, 罗艳蕊, 范红军, 等. 离子液体[C8mim]. Br对不同日龄大型溞的急性毒性[J]. 生态环境学报, 2008, 17(3):1021-1023.

    ZHANG B J, LUO Y X, FAN H J, et al. The acute toxicity of 1 methyl-3-octylimidazolium bromide to Daphnia magna at different developmental stages[J]. Ecology and Environment, 2008, 17(3):1021-1023(in Chinese).

    [27] 李秀环, 李华, 陈澄宇, 等. 不同种类农药表面活性剂对大型溞的急性毒性[J]. 应用生态学报, 2013, 24(8):2319-2324.

    LI X H, LI H, CHEN C Y, et al. Acute toxicity of different type pesticide surfactants to Daphnia magna[J]. Chinese Journal of Applied Ecology, 2013, 24(8):2319-2324(in Chinese).

    [28] BERNOT R J, BRUESEKE M A, EVANS-WHITE M A, et al. Acute and chronic toxicity of imidazolium-based ionic liquids on Daphnia magna[J]. Environmental Toxicology and Chemistry, 2005, 24(1):87-92.
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  • 收稿日期:  2019-08-16

利用大型溞评估离子液体[C8mim]Cl生物毒性初探

    通讯作者: 邓祥元, E-mail: dengxy2016@foxmail.com
  • 江苏科技大学生物技术学院, 镇江, 212018
  • 收稿日期:  2019-08-16
基金项目:

江苏省第十五批"六大人才高峰"高层次人才选拔培养项目（SWYY-025）和镇江市重点研发计划项目（SH2019004）资助.

摘要: 为评估1-辛基-3-甲基咪唑氯盐（[C8mim]Cl）的生物毒性，本研究以大型溞（Daphnia magna）为实验对象，通过急性毒性实验分析了不同日龄大型溞对[C8mim]Cl的敏感性，并以存活率、产溞能力和蜕皮次数为指标研究了[C8mim]Cl对大型溞的慢性毒性效应，这将为评估离子液体的毒性效应提供科学依据.结果表明，[C8mim]Cl对1、2、4日龄大型溞的48 h半致死浓度（LC50）分别为208.9、182.7、178.6 μg·L-1，表明随着大型溞日龄的增加，[C8mim]Cl对大型溞的毒性效应逐渐增大并趋于稳定.此外，[C8mim]Cl对大型溞生活史中的初次产溞时间、总产溞数目、总产溞次数和蜕皮次数等影响不显著，但显著影响大型溞的存活率和初次产溞个数，因此，大型溞的存活率和初次产溞个数是评估[C8mim]Cl对大型溞慢性毒性效应的两个较为敏感的指标.

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