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高效液相色谱串联质谱法测定鱼体内双酚A及其替代品

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杨丽萍, 王强, 祝凌燕. 高效液相色谱串联质谱法测定鱼体内双酚A及其替代品[J]. 环境化学, 2018, 37(8): 1842-1850. doi: 10.7524/j.issn.0254-6108.2018031602
引用本文: 杨丽萍, 王强, 祝凌燕. 高效液相色谱串联质谱法测定鱼体内双酚A及其替代品[J]. 环境化学, 2018, 37(8): 1842-1850. doi: 10.7524/j.issn.0254-6108.2018031602
shu
YANG Liping, WANG Qiang, ZHU Lingyan. Simultaneous determination of Bisphenol A and its alternatives in fish samples by ultra performance liquid chromatography electrospray tandem mass spectrometry[J]. Environmental Chemistry, 2018, 37(8): 1842-1850. doi: 10.7524/j.issn.0254-6108.2018031602
Citation: YANG Liping, WANG Qiang, ZHU Lingyan. Simultaneous determination of Bisphenol A and its alternatives in fish samples by ultra performance liquid chromatography electrospray tandem mass spectrometry[J]. Environmental Chemistry, 2018, 37(8): 1842-1850. doi: 10.7524/j.issn.0254-6108.2018031602
shu

高效液相色谱串联质谱法测定鱼体内双酚A及其替代品

  • 1.

    环境污染过程与基准教育部重点实验室, 天津市城市生态环境修复与防治重点实验室, 南开大学环境科学与工程学院, 天津, 300350

  • 基金项目:

    国家自然科学基金（21577067，21737003）和教育部111创新引智基地计划（T2017002）资助.

Simultaneous determination of Bisphenol A and its alternatives in fish samples by ultra performance liquid chromatography electrospray tandem mass spectrometry

  • 1.

    Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education, Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin, 300350, China

  • Fund Project: Supported by the National Natural Science Foundation of China (21577067, 21737003) and Ministry of Education 111 Innovative Intelligence Base Plan of China(T2017002).

  • 摘要: 为实现生物体中双酚A及其替代物的准确、快速、高灵敏度的同时测定，采用固相萃取与超高效液相色谱和三重四极杆质谱联用（SPE-UPLC-MS/MS）技术，建立了测定鱼体内双酚A及其8种替代物（双酚B（BPB）、双酚C（BPC）、双酚E（BPE）、双酚F（BPF）、双酚S（BPS）、双酚Z（BPZ）、双酚AF（BPAF）和双酚AP（BPAP））的前处理和分析方法.结果显示，以V（甲醇）：V（水）=1：1的混合液为萃取剂提取鱼体中的双酚类目标物，经过HC-C18 SPE（500 mg）柱净化富集后，采用Waters BEH Shield RP18（2.1×150 mm，1.7 μm，Waters）色谱柱分离目标物，9种目标双酚类化合物的线性范围0.500-100 ng·g-1（r > 0.995），检出限可达0.100-380 pg·g-1湿重，回收率范围为68.0%-125%，相对标准偏差范围为1.4%-5.7%.通过分析锦鲤全鱼样品，证明该方法样品回收率高、检出限低、灵敏度高、重现性好，具有较好的实用性.
    Abstract: In order to determine bisphenol A and its alternatives accurately, rapidly and sensitively, a method for the sample pretreatment and determination of bisphenol A and eight alternatives(bisphenol B(BPB)、bisphenol C(BPC)、bisphenol E(BPE)、bisphenol F(BPF)、bisphenol S(BPS)、bisphenol Z(BPZ)、bisphenol AF(BPAF)and bisphenol AP(BPAP)) in fish was established, which coupled ultra performance liquid chromatography with triple quadruples mass spectrometry. The optimum sample pretreatment conditions were established as follows V(methanol):V(water)=1:1 mixed solution as extractant, HC-C18 SPE cartridge for purification and enrichment. The target compounds were separated on a Waters BEH Shield R18 column. The linear range of the nine target bisphenol compounds was 0.500-100 ng·g-1 (r > 0.995) and the detection limit was 0.100-380 pg·g-1 wet weight (ww). The recovery of the nine target bisphenol compounds was in the range of 68.0%-125%, and the relative standard deviation was 1.4%-5.7%. The developed method was validated using common carp exposed to the target compounds. The method was proved to be a reliable and cost-effective alternative technique for the analysis of lowers level of bisphenol compounds in biota.
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    [2] BRAUN J M. Early-life exposure to EDCs:role in childhood obesity and neurodevelopment[J]. Nature Reviews Endocrinology, 2016, 13:161-173.
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  • 收稿日期:  2018-03-16
  • 刊出日期:  2018-08-15
杨丽萍, 王强, 祝凌燕. 高效液相色谱串联质谱法测定鱼体内双酚A及其替代品[J]. 环境化学, 2018, 37(8): 1842-1850. doi: 10.7524/j.issn.0254-6108.2018031602
引用本文: 杨丽萍, 王强, 祝凌燕. 高效液相色谱串联质谱法测定鱼体内双酚A及其替代品[J]. 环境化学, 2018, 37(8): 1842-1850. doi: 10.7524/j.issn.0254-6108.2018031602
shu
YANG Liping, WANG Qiang, ZHU Lingyan. Simultaneous determination of Bisphenol A and its alternatives in fish samples by ultra performance liquid chromatography electrospray tandem mass spectrometry[J]. Environmental Chemistry, 2018, 37(8): 1842-1850. doi: 10.7524/j.issn.0254-6108.2018031602
Citation: YANG Liping, WANG Qiang, ZHU Lingyan. Simultaneous determination of Bisphenol A and its alternatives in fish samples by ultra performance liquid chromatography electrospray tandem mass spectrometry[J]. Environmental Chemistry, 2018, 37(8): 1842-1850. doi: 10.7524/j.issn.0254-6108.2018031602
shu

高效液相色谱串联质谱法测定鱼体内双酚A及其替代品

  • 1. 环境污染过程与基准教育部重点实验室, 天津市城市生态环境修复与防治重点实验室, 南开大学环境科学与工程学院, 天津, 300350
  • 收稿日期:  2018-03-16
基金项目:

国家自然科学基金（21577067，21737003）和教育部111创新引智基地计划（T2017002）资助.

摘要: 为实现生物体中双酚A及其替代物的准确、快速、高灵敏度的同时测定，采用固相萃取与超高效液相色谱和三重四极杆质谱联用（SPE-UPLC-MS/MS）技术，建立了测定鱼体内双酚A及其8种替代物（双酚B（BPB）、双酚C（BPC）、双酚E（BPE）、双酚F（BPF）、双酚S（BPS）、双酚Z（BPZ）、双酚AF（BPAF）和双酚AP（BPAP））的前处理和分析方法.结果显示，以V（甲醇）：V（水）=1：1的混合液为萃取剂提取鱼体中的双酚类目标物，经过HC-C18 SPE（500 mg）柱净化富集后，采用Waters BEH Shield RP18（2.1×150 mm，1.7 μm，Waters）色谱柱分离目标物，9种目标双酚类化合物的线性范围0.500-100 ng·g-1（r > 0.995），检出限可达0.100-380 pg·g-1湿重，回收率范围为68.0%-125%，相对标准偏差范围为1.4%-5.7%.通过分析锦鲤全鱼样品，证明该方法样品回收率高、检出限低、灵敏度高、重现性好，具有较好的实用性.

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