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磺胺与群感抑制剂对大肠杆菌的联合毒性机制初探

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刘灿, 葛鸿铭, 龙茜, 安情情, 林志芬, 印春生. 磺胺与群感抑制剂对大肠杆菌的联合毒性机制初探[J]. 环境化学, 2015, 34(10): 1854-1859. doi: 10.7524/j.issn.0254-6108.2015.10.2015042001
引用本文: 刘灿, 葛鸿铭, 龙茜, 安情情, 林志芬, 印春生. 磺胺与群感抑制剂对大肠杆菌的联合毒性机制初探[J]. 环境化学, 2015, 34(10): 1854-1859. doi: 10.7524/j.issn.0254-6108.2015.10.2015042001
shu
LIU Can, GE Hongming, LONG Xi, AN Qingqing, LIN Zhifen, YIN Chunsheng. coli, 群体感应抑制剂(QSI), 磺胺类抗生素, 联合毒性. A preliminary investigation on the mechanism of binary mixture toxicity containing sulfonamides and quorum sensing inhibitors on Escherichia coli[J]. Environmental Chemistry, 2015, 34(10): 1854-1859. doi: 10.7524/j.issn.0254-6108.2015.10.2015042001
Citation: LIU Can, GE Hongming, LONG Xi, AN Qingqing, LIN Zhifen, YIN Chunsheng. coli, 群体感应抑制剂(QSI), 磺胺类抗生素, 联合毒性. A preliminary investigation on the mechanism of binary mixture toxicity containing sulfonamides and quorum sensing inhibitors on Escherichia coli[J]. Environmental Chemistry, 2015, 34(10): 1854-1859. doi: 10.7524/j.issn.0254-6108.2015.10.2015042001
shu

磺胺与群感抑制剂对大肠杆菌的联合毒性机制初探

  • 1.

    上海海洋大学海洋学院, 上海, 201306;

  • 2.

    污染控制与资源化研究国家重点实验室, 同济大学环境科学与工程学院, 上海, 200092

  • 基金项目:

    同济大学污染控制与资源化研究国家重点实验室自主研究项目(PCRRY11003)

    国家自然科学基金(201177092,21377096)

    中央高校基本科研业务费专项资金(0400219181)资助.

coli, 群体感应抑制剂(QSI), 磺胺类抗生素, 联合毒性. A preliminary investigation on the mechanism of binary mixture toxicity containing sulfonamides and quorum sensing inhibitors on Escherichia coli

  • 1.

    College of Marine Science, Shanghai Ocean University, Shanghai, 201306, China;

  • 2.

    State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China

  • Fund Project:

  • 摘要: 以大肠杆菌(Escherichia coli, E.coli)为模式生物, 磺胺及3种群体感应抑制剂(QSI)为研究对象, 测定其单一毒性和联合毒性, 采用分子对接技术初步探索了磺胺和QSI对E.coli的单一及联合毒性效应机制.结果显示, 其联合毒性效应多表现为拮抗, 推断可能是由于磺胺促进E.coli的SdiA蛋白的表达, 后者与QSIs结合, 减少其生物有效剂量, 导致拮抗效应.该研究为抗生素联用及抗生素的环境联合生态风险评价提供一定的理论依据和技术支持.
    Abstract: Sulfonamides and three quoram sensing inhibitors (QSIs) were chosen to study the single and mixture toxicites on E.coli. The single and mixture toxicities of sulfonamides and QSIs were determined using E.coli as a mode organism, and the mechanism of the joint effects between sulfonamides and QSIs was explored with the method of Molecular Docking. The results showed that the joint effects were mostly antagonistic.This is probably because sulfonamides could promote the expression of SdiA, with which QSIs were assumed to bind, leading to the decrease of QSIs consequently.
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出版历程
  • 收稿日期:  2015-04-20
  • 刊出日期:  2015-10-15
刘灿, 葛鸿铭, 龙茜, 安情情, 林志芬, 印春生. 磺胺与群感抑制剂对大肠杆菌的联合毒性机制初探[J]. 环境化学, 2015, 34(10): 1854-1859. doi: 10.7524/j.issn.0254-6108.2015.10.2015042001
引用本文: 刘灿, 葛鸿铭, 龙茜, 安情情, 林志芬, 印春生. 磺胺与群感抑制剂对大肠杆菌的联合毒性机制初探[J]. 环境化学, 2015, 34(10): 1854-1859. doi: 10.7524/j.issn.0254-6108.2015.10.2015042001
shu
LIU Can, GE Hongming, LONG Xi, AN Qingqing, LIN Zhifen, YIN Chunsheng. coli, 群体感应抑制剂(QSI), 磺胺类抗生素, 联合毒性. A preliminary investigation on the mechanism of binary mixture toxicity containing sulfonamides and quorum sensing inhibitors on Escherichia coli[J]. Environmental Chemistry, 2015, 34(10): 1854-1859. doi: 10.7524/j.issn.0254-6108.2015.10.2015042001
Citation: LIU Can, GE Hongming, LONG Xi, AN Qingqing, LIN Zhifen, YIN Chunsheng. coli, 群体感应抑制剂(QSI), 磺胺类抗生素, 联合毒性. A preliminary investigation on the mechanism of binary mixture toxicity containing sulfonamides and quorum sensing inhibitors on Escherichia coli[J]. Environmental Chemistry, 2015, 34(10): 1854-1859. doi: 10.7524/j.issn.0254-6108.2015.10.2015042001
shu

磺胺与群感抑制剂对大肠杆菌的联合毒性机制初探

  • 1.  上海海洋大学海洋学院, 上海, 201306;
  • 2.  污染控制与资源化研究国家重点实验室, 同济大学环境科学与工程学院, 上海, 200092
  • 收稿日期:  2015-04-20
基金项目:

同济大学污染控制与资源化研究国家重点实验室自主研究项目(PCRRY11003)

国家自然科学基金(201177092,21377096)

中央高校基本科研业务费专项资金(0400219181)资助.

摘要: 以大肠杆菌(Escherichia coli, E.coli)为模式生物, 磺胺及3种群体感应抑制剂(QSI)为研究对象, 测定其单一毒性和联合毒性, 采用分子对接技术初步探索了磺胺和QSI对E.coli的单一及联合毒性效应机制.结果显示, 其联合毒性效应多表现为拮抗, 推断可能是由于磺胺促进E.coli的SdiA蛋白的表达, 后者与QSIs结合, 减少其生物有效剂量, 导致拮抗效应.该研究为抗生素联用及抗生素的环境联合生态风险评价提供一定的理论依据和技术支持.

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